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Recorded Webinars

Genome Editing

Genome Editing

Title: Genome Editing: How Do I Use CRISPR?

Presented Wednesday, February 22, 2017

Genome Editing-the ability to make specific changes at targeted genomic sites-is fundamentally important to researchers in biology and medicine. CRISPR is a very widely-used method for modifying specific genome sites, and can be used for many applications, including gene knock out, transgene knock in, gene tagging, and correction of genetic defects. However, researchers are often unaware of some of the work required to identify their desired modification in their cell lines. In this webinar, we discuss what you need to do for CRISPR genome editing after you have obtained your reagents from GeneCopoeia, the so-called “Downstream work”.

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Title: GeneCopoeia CRISPR Genome Editing Technology

Presented Wednesday, January 25, 2017

The ability to make specific changes at targeted genomic sites in complex organisms is fundamentally important to researchers in biology and medicine. Researchers have developed and refined chimeric DNA endonucleases, such as CRISPR-Cas9, to stimulate double strand breaks at defined genomic loci, allowing the ability to insert, delete, and replace genetic information at will. These tools can also be used without nucleases to induce or repress gene transcription. In this webinar, we discuss CRISPR and other genome editing technologies and the applications they make possible, and provide information on GeneCopoeia's powerful suite of genome editing products and services.

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Title: Applications For CRISPR-Cas9 Stable Cell Lines

Presented Wednesday, March 22, 2017

The CRISPR-Cas9 system has become greatly popular for genome editing in recent years, due to its ease-of-design, efficiency, specificity, and relatively low cost. In mammalian cell culture systems, most genome editing is achieved using transient transfection or lentiviral transduction, which works well for routine, low-throughput applications. However, for other applications, it would be beneficial to have a system in which one component, namely the CRISPR-Cas9 nuclease, was stably integrated into the genome. In this webinar, we introduce GeneCopoeia’s suite of Cas9 stable cell lines, and discuss the great utility that these cell lines provide for genome editing applications.

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Title: Safe Harbor Transgenesis in Human & Mouse Genome Editing

Presented Wednesday, April 19, 2017

Insertion of transgenes in mammalian chromosomes is an important approach for biomedical research and targeted gene therapy. Traditional lentiviral-mediated transgenesis is effective and straightforward, but its random integration can often be unstable and harm cells. "Safe Harbor" sites in human and mouse chromosomes have been employed recently as an alternative to random, viral-mediated integration because they support consistent, stable expression, and are not known to hamper cell fitness or growth. In this webinar, we will discuss the merits of Safe harbor transgenesis approaches, and how GeneCopoeia's CRISPR tools for Safe Harbor knock-in can greatly benefit your research.

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Title: GeneCopoeia CRISPR sgRNA Libraries For Functional Genomics

Presented Wednesday, April 29, 2015

Biomedical researchers are enjoying a Renaissance in functional genomics, which aims to use a wealth of DNA sequence information—most notably, the complete sequence of the human genome—to determine the natural roles of the genes encoded by the genome. As a result, biochemical networks and pathways will be better understood, with the hope of leading to improved disease treatments. Researchers are turning increasingly to CRISPR (clustered, regularly interspaced, short palindromic repeats) for functional genomics studies.  Several groups recently adapted CRISPR for high-throughput knockout applications, by developing large-scale CRISPR sgRNA libraries. GeneCopoeia recently launched a number of smaller, pathway- and gene group-focused CRISPR sgRNA libraries, which offer several key advantages over the whole-genome libraries. In this 40 minute webinar, we discuss the merits and applications for CRISPR sgRNA libraries, how to use CRISPR sgRNA libraries, the advantages of using small, pathway- and gene group-focused libraries, and how GeneCopoeia can help you with your high-throughput CRISPR knockout studies.

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Viral systems

Viral systems

Title: How To Choose Between Lentivirus and Adeno-associated Virus For DNA Delivery

Presented Wednesday, April 12, 2017

Delivery of exogenous DNA to cultured cells and animals is a fundamental strategy for modern molecular biology, functional genomics, and gene therapy. While plasmid transfection is widely used for routine DNA delivery into cultured cells, the use of viral vectors is often advantageous, especially for cells that are refractory to transfection. Further, viral vectors remain the only practical option for DNA delivery to tissues in vivo. In this webinar, we will compare lentiviral systems with adeno-associated virus (AAV) systems, how to decide which system is appropriate for a specific scenario, and how GeneCopoeia’s powerful suite of lentiviral and AAV products and services can help you with your DNA delivery applications.

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Cell Biology

 

Cell Biology

Title: ‘Till Death Do Us Part: GeneCopoeia Solutions For Apoptosis Assays

Presented Thursday, December 1, 2016

Apoptosis is the process of programmed cell death, and is readily distinguishable from necrosis. Apoptosis is essential for the development and maintenance of multicellular organsisms, and its dysregulation can lead to diseases such as cancers. In this webinar, we discuss some of the hallmarks of apoptosis, and GeneCopoeia's comprehensive suite of reagents, kits, and clones designed to study all aspects of apoptosis.

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Promoter-reporter clones

 

Promoter-reporter clones

Title: Improved Promoter Reporter Technology For Understanding Gene Regulation From GeneCopoeia

Presented Wednesday, September 10, 2014

Identifying and characterizing DNA regulatory elements is critical for determining gene expression patterns. Typically, the strength of expression of reporters such as GFP or firefly luciferase is measured in response to environmental conditions, or to stimuli by growth factors or pharmacological agents. However, these assays are often laborious and time consuming, due to the requirement for fluorescence microscopy or cell lysis for reporter detection. In this 30 minute webinar, you will learn how GeneCopoeia provides improved promoter reporter detection technology through the use of Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) in a dual reporter assay system. Both reporters are secreted by cells, and can be assayed in live cells quickly and conveniently.

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ORF clones

ORF clones

Title: Functional Tags For ORF Clones in Your Research

Presented Wednesday, February 19, 2014

Overexpression of proteins is commonly performed in functional genomics, proteomics and system biology studies. Fusing oligopeptide or protein tags to proteins is a popular strategy for the detection and purification of proteins when a suitable antibody recognizing the native protein is unavailable. GeneCopoeia is the original manufacturer of human and mouse expression-ready, full-length ORF cDNA clones. These clones are available with more than 50 classical, popular tags, as well as powerful multifunctional tags. In addition, GeneCopoeia is the only company with a comprehensive set of native expression clones. In this 45 minute webinar, we will tell you about the utility of the more powerful, next generation tags, and how GeneCopoeia's extensive collection of tagged and native expression ready clones can greatly accelerate your research.

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