You are here: Home > Products > Clone collections >

miArrest™ miRNA Inhibitors

 

Introduction

microRNA (miRNA) inhibitors block miRNA regulation of target gene expression. They are designed and optimized for miRNA loss of function study. GeneCopoeia offers miArrest miRNA inhibitors as vector-based expression clones or synthetic oligonucleotides.

 

Vector-based expression clones are available in lentiviral and non-viral vectors. miRNA inhibitor clones bind specifically to their target miRNAs allowing transient as well as stable suppression of the target gene. The choice of H1 or U6 promoter allows constitutive expression of inhibitors in virtually all types of mammalian cells.

 

 

 Advantages

Full coverage

  • All known human, mouse and rat miRNAs in the miRBase covered.

Flexible delivery systems

  • Lentiviral vectors allow efficient transduction and stable integration into the host genome of non-dividing and difficult to transfect cells target cells
  • Non-viral vectors allow either transient or stable transfection

Advanced and reliable design

  • Expression cassettes have been designed using a proprietary algorithm and tested rigorously by carefully designed experiments

Superior performance

  • Superior potency, long lasting inhibition and extremely low cell toxicity compared to other chemically synthesized miRNA inhibitors (see table 1)
  • Choices of H1 or U6 promoter allow constitutive expression of inhibitors in virtually all mammalian cell types.

High-throughput compatible

  • Quick and easy assay format
  • High sample number compatible

 

Figure 1. Data showing the effect of miArrest miRNA inhibitor clone in a dose-dependent manner A miR-125a inhibitor expression plasmid (GeneCopoeia HmiR-AN0094-AM01) was transfected into HEK 293 cells with 1) a miR-125a precursor expression plasmid (GeneCopoeia HmiR0309-MR03) and 2) a miRNA target sequence expression clone expressing LIN28, a known target gene for miR-125a (GeneCopoeia HmiT019205-MT02: 3'-UTR sequence of LIN28 in gaussia luciferase-alkaline phosphatase dual reporter expression vector). Both the GLuc activity and an internal control AP activity were determined 24 hours post-transfection. The activity ratio of GLuc to AP was set to 1 for the single transfection sample with LIN28 3'-UTR target sequence expression clone (left-most bar), against which the activities of other samples were normalized. The result shows that mir-125a suppressed the luciferase activity from the Gluc-LIN28-3'-UTR clone by more than 70% (Bar 2 from left). This suppression effect was blocked by the introduction of varying amount of miArrest™ inhibitor clone against miR-125a in a dose-dependent manner. At the highest dose, the reporter GLuc activity is higher than the control (right-most bar). This could be attributed to the fact that this vector-based inhibitor may have blocked the regulatory effect of endogenous miR-125a, which would result in increased translational activity of GLuc-LIN28-3'-UTR transcript.

 

 
  Vector-Based Inhibitors Synthetic 2'-OMe Inhibitors LNA Inhibitors
Inhibition +++++ ++ ++
Specificity +++++ +++ +++
Stability +++++ + +++
Durability Long term Transient Transient
Cell Toxicity - - +
Delivery to resting and hard-to-transfect cells +++++ - -
Table 1. Comparison of vector-based inhibitors vs. synthesized miRNA inhibitors

 

 

Figure 2. Simplified mechanism of action for vector-based miRNA inhibitors

miRNA inhibitor constructs bind specifically to their target miRNA upon transduction into cells. The post-transcriptional processing causes formation of an entrapping structure that attracts and binds to two molecules of the intended miRNA.

This in turn prevents the binding of endogenous miRNA to target mRNA, thus inhibiting the effect of miRNA on target gene and facilitating enhanced target gene expression (figure 3).

A unique vector design ensures maximum inhibition with specificity and long-lasting stability while keeping off-target effects to a minimum.

 

 

Vectors           

miRNA inhibitor vector features                                                         

Vector Reporter gene Selection marker Promoter Type
pEZX-AM01 mCherry Puromycin H1 Mammalian
pEZX-AM02 mCherry Puromycin U6 Mammalian
pEZX-AM03 mCherry Hygromycin H1 HIV
pEZX-AM04 mCherry Hygromycin U6 HIV

 

miRNA inhibitor lentivirus

 

GeneCopoeia offers precursor miRNA lentivirus as a custom service for only $995 and delivered within 2-5 weeks.

 

miRNA synthetic inhibitor

Designed and optimized to bind to specific miRNA molecules. Learn more

 

Clone Sets

miRNA inhibitor clone sets

Besides individual clones, GeneCopoeia also offers pre-made or custom-made miRNA inhibitor clone sets. Uniformity and high-quality clone sets make them ideal for large-scale high content and high-throughput screening studies in functional genomics, proteomics and systems biology.

 

Vector choices

Vector Vector Type Promoter Selection Marker Tag
pEZX-AM03 Mammalian expression (lentiviral) H1 Hygromycin mCherry

 

Clone sets by gene family in the vector of your choice

Please contact us at 1-866-360-9531 or inquiry@genecopoeia.com if you have any questions.

Vector and species:

More vector types are available

 

Human whole genome miRNA inhibitor clone set (Lentiviral)

Cat. No. Species Product miRNA Price
MIC-HG-AM03 Human Human whole genome miRNA  inhibitor clone set (lentiviral) 1177 Inquire
Custom order Various Custom-selected Various Inquire

 

Human cancer miRNA inhibitor clone sets (Lentiviral)

Cat.No. Species Product Cancer miRNA Price
MIC-HCY-AM03 Human  Human bladder cancer miRNA inhibitor clone set Bladder cancer 63 Inquire
MIC-HCN-AM03 Human  Human brain cancer miRNA inhibitor clone set Brain cancer 206 Inquire
MIC-HCB-AM03 Human  Human breast cancer miRNA inhibitor clone set Breast cancer 158 Inquire
MIC-HCV-AM03 Human  Human cervical cancer miRNA inhibitor clone set Cervical cancer 26 Inquire
MIC-HCI-AM03 Human  Human cholangiocarcinoma miRNA inhibitor clone set Cholangiocarcinoma 25 Inquire
MIC-HCC-AM03 Human  Human colorectal cancer miRNA inhibitor clone set Colorectal cancer 175 Inquire
MIC-HCW-AM03 Human  Human endometrial cancer miRNA inhibitor clone set Endometrial cancer 86 Inquire
MIC-HCG-AM03 Human  Human gastric cancer miRNA inhibitor clone set Gastric cancer 76 Inquire
MIC-HCT-AM03 Human  Human head and neck cancer miRNA inhibitor clone set Head and Neck cancer 188 Inquire
MIC-HCH-AM03 Human  Human hepatocellular carcinoma miRNA inhibitor clone set Hepatocellular Carcinoma 170 Inquire
MIC-HCK-AM03 Human  Human kidney cancer miRNA inhibitor clone set Kidney cancer 67 Inquire
MIC-HCX-AM03 Human  Human leukemia miRNA inhibitor clone set Leukemia 163 Inquire
MIC-HCL-AM03 Human  Human lung cancer miRNA inhibitor clone set Lung cancer 178 Inquire
MIC-HCZ-AM03 Human  Human lymphoma miRNA inhibitor clone set Lymphoma 112 Inquire
MIC-HCM-AM03 Human  Human melanoma miRNA inhibitor clone set Melanoma 131 Inquire
MIC-HCE-AM03 Human  Human mesothelioma miRNA inhibitor clone set Mesothelioma 21 Inquire
MIC-HCR-AM03 Human  Human myeloma miRNA inhibitor clone set Myeloma 45 Inquire
MIC-HCO-AM03 Human  Human ovarian cancer miRNA inhibitor clone set Ovarian cancer 174 Inquire
MIC-HCP-AM03 Human  Human pancreatic cancer miRNA inhibitor clone set Pancreatic cancer 166 Inquire
MIC-HCA-AM03 Human  Human pituitary adenoma miRNA inhibitor clone set Pituitary Adenoma 33 Inquire
MIC-HCQ-AM03 Human  Human prostate cancer miRNA inhibitor clone set Prostate cancer 153 Inquire
MIC-HCS-AM03 Human  Human sarcoma miRNA inhibitor clone set Sarcoma 27 Inquire
MIC-HCJ-AM03 Human  Human thyroid carcinoma miRNA inhibitor clone set Thyroid Carcinoma 55 Inquire
MIC-HCU-AM03 Human  Human uterine leiomyoma miRNA inhibitor clone set Uterine Leiomyoma 40 Inquire
Custom order Various Custom-selected Various Various Inquire
Cat. No. Species Product miRNA Price
MIC-MG-AM03 Mouse Mouse genome miRNA inhibitor clone set (lentiviral) 331 Inquire
Custom order Various Custom-selected Various Inquire
Cat. No. Species Product miRNA Price
MIC-RG-AM03 Rat Rat genome miRNA inhibitor clone set (lentiviral) 241 Inquire
Custom order Various Custom-selected Various Inquire

 

Related Products

Publications

2017

  • Jiang, X., et al. (2017). Overexpression of miR-92a promotes the tumor growth of osteosarcoma by suppressing F-box and WD repeat-containing protein 7. Gene doi: 10.1016/j.gene.2017.01.002. [miArrest™ miR-92a inhibitor clone]
  • Men, R., et a. (2017). MircoRNA-145 promotes activation of hepatic stellate cells via targeting krüppel-like factor 4. Scientific Reports doi: 10.1038/srep40468 [miArrest™ miR-145 inhibitor clone]
  • Hou, S., et al. (2017). MicroRNA-939 governs vascular integrity and angiogenesis through targeting γ-catenin in endothelial cells. Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2017.01.085 [miArrest™ miR-939 inhibitor clone]

2016

 

2015

 

2014

 

2013

 

< 2012

 

View more miRNA publications