Luc-Pair™ Firefly Luciferase HS Assay Kit

*50% Off Luc-Pair™ Luciferase Kits

High sensitivity

 

Introduction

GeneCopoeia's Luc-Pair™ Firefly Luciferase Assay Kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) activity. The Luc-Pair™ Firefly Luciferase HS Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) using micro-plate or single-tube luminometers, and is compatible with GeneCopoeia, Promega and many other Fluc vectors.

Advantages

  • High sensitivity-The reagents have been developed so that the signals for Firefly luciferase exhibit greatest sensitivity (Figure 1).
  • Versatility-The system has been designed for assays with many different eukaryotic (vertebrates, lower invertebrates) cells using micro-plates or single-tube luminescence reader.
  • Low background-The system produces very limited background luminescence. No subtraction is required from readings.
  • Reproducibility-This system is designed to yield reliable, linear results for a concentration range over several orders of magnitude.

 

 

Buy Catalog# Product Description Price
LF007 Luc-Pair™ Firefly Luciferase HS Assay Kit (100rxns) 

High sensitivity for detecting the activity of Firefly luciferase.

$77
LF008 Luc-Pair™ Firefly Luciferase HS Assay Kit (300rxns) $125
LF009 Luc-Pair™ Firefly Luciferase HS Assay Kit (1000rxns) $375
LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $40

  User Manual MSDS

 

GeneCopoeia also offers the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (for enhanced stability) and  Luc-Pair™ Duo-Luciferase HS Assay Kit (for high sensitivity) for applications requiring luciferase readings.

 

Figure 1.  Activity of Firefly luciferase signals using GeneCopoeia Luc-Pair™ Firefly Luciferase HS Assay Kit. HEK 293 cells were transfected with Promega pGL4.13 reporter vector for 48 hours. The FLuc (FLuc-GeneCopoeia) activity was measured as described in the procedure. Promega’s Luciferase Assay System was used (FLuc-Promega) in comparison.

 

           

Figure 2.  Signal stability of luciferase using GeneCopoeia Luc-Pair Firefly-Luciferase (FLuc) HS Assay Kit. HEK 293 cells were transfected with Promega pGL4.13 reporter vector for 48 hours. The FLuc (FLuc-GeneCopoeia) activity was measured as described in the procedure. A sample injector was used to dispense the Fluc Working Solution, set shaking to mix for 1 sec, and counting was started immediately for 20 sec.

 

*Discount offer is valid for 300rxns and 1000rxns only. Not valid for previous purchases. Offer expires on June 30th, 2017.