GeneCopeia provides transgenic mouse services using CRISPR-Cas9. Many applications are readily within reach, including constitutive and conditional gene knockout, mutagenesis, correction of disease mutations, transgene knockin, and in-frame fusion tagging.
Case study: Gene tagging using GeneCopoeia's CRISPR transgenic mouse services
|Figure 2. Site-specific genome editing mediated by TALEN or CRISPR-Cas9, as well as its application on transgenic mouse generation.|
1. Knockout mice by TALEN-mediated gene targeting.Nature Biotrchnology.2012,31,23-24
2. Generation of gene-modified mice via Cas9/RNA-mediated gene targeting.Cell Research.2013,23,720-723.
3. One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Geneme Engineeing.Cell 2013,153,1-9.
Mutagenesis or correction of disease mutations
Knockin mouse models can incorporate mutations into a target region, or correct disease-causing mutations. This is readily achievable using CRISPR-Cas9 and an oligonucleotide donor for HR.
We provide mouse lines with permanent, germline-transmissible knockouts, knockins and transgenes of interest. These modifications, which are produced using CRISPR-Cas9, are delivered as heterozygous F1 mice. GeneCopoeia also offers genome-editing consulting services. To get a quote, please fill out the inquiry form.
- 3 or more F1 positive mice with TALEN- or CRISPR-Cas9-mediated trangenic modifications
- Service report
Estimated Time of Arrival
- TALEN- or CRISPR-Cas9-mediated trangenic mice: 6-9 months
Genome-CRISP™ CRISPR-Cas9 products and services – GeneCopoeia offers CRISPR-Cas9 sgRNA design and cloning services, Cas9 expression clones and other related products and services for simple and fast targeted genomic editing.
Genome-TALER™ TALEN and TALE-TF custom services – GeneCopoeia offers several levels of affordable custom services for designing, creating and validating TALENs, TALE-TFs and other TAL effectors-based targeted genomic modification tools.
Human AAVS1 & Mouse ROSA26 safe harbor gene knockin kits and clones – TALEN- or CRISPR-mediated gene knock-in kits designed to specifically transfer your gene of interest, selection marker or other genetic element into the human AAVS1 or mouse ROSA26 safe harbor site . More than 45,000 ROSA26 or AAVS1 compatible ORF donor clones are also available. The safe integration ensures transcription-competency of the transgenes and presents no known adverse effect.