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AAVPrime™ Adeno-associated Viral (AAV) Particles

Introduction

GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for inserting genes into a broad range of cell types with high efficiency and enhanced safety. GeneCopoeia’s optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made particle options are available in 7 different serotypes, including fluorescent reporters (Figure 1), or you can have us produce custom AAV particles for you with genes up to 3 kb in length. Particle preparations are available in either standard (for in vitro use only; Figure 1) or purified (suitable for in vivo use; Figure 2) formats.

Advantages

  • High titers. Titer of purified particles can be up to 10^14 GC/ml (genome copies/ml)
  • Versatile. Usable in a broad range of host cell types
  • Low toxicity. Does not integrate into the host genome.
  • Low immunogenicity. Minimal host immune response.
  • High adaptability. All serotypes are available.
  • Safe. Not associated with any human disease

 

GeneCopoeia's AAVPrime™ standard AAV particles expressing fluorecent reporters

A B C
图片A 图片B 图片C

Figure 1. HT1080 cells in 24-well plates were transduced with 0.5 µL of standard purity AAV particles expressing either eGFP (A), RFP (B), or mCherry (C)). Cells were visualized with a fluorescence microscope (Exposure time: 400 ms).

 

GeneCopoeia's AAVPrime™ purified AAV particles expressing eGFP

0.005 μl 0.05 μl 0.5 μl

Figure 2. HT1080 cells in 24-well plates were transduced with the indicated amounts of purified AAV particles expressing eGFP. Cells were visualized with a fluorescence microscope (Exposure time: 400 ms).

To Order

Order custom AAV particles

Custom AAV particles production services are currently available for ORF expression. GeneCopoeia' s scientific expertise can deliver AAV particles with titers starting from 10^11 GC/mL or higher depending upon the request.

For all AAV particles not covered in the premade particles collection, request a custom quote here.

 

Order premade AAV particles

By default, all options for AAV particle serotype, purity level (standard or purified) and volume are listed in the table below. Use the drop-down menus to narrow the number of options displayed.

Serotype: Grade: Size:

Buy Catalog Size Product Description Gene of Interest Type Price
AA002100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing eGFP.
Serotype: 2.   Promoter: CMV.
eGFPPurified$500
AA003500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing eGFP.
Serotype: 2.   Promoter: CMV.
eGFPPurified$650
AA008500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing eGFP.
Serotype: 2.   Promoter: CMV.
eGFPStandard$300
AA0091ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing eGFP.
Serotype: 2.   Promoter: CMV.
eGFPStandard$400
AA012100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: 2.   Promoter: CMV.
IRES-hrGFPPurified$500
AA013500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: 2.   Promoter: CMV.
IRES-hrGFPPurified$650
AA018500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: 2.   Promoter: CMV.
IRES-hrGFPStandard$300
AA0191ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: 2.   Promoter: CMV.
IRES-hrGFPStandard$400
AA022100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing LacZ.
Serotype: 2.   Promoter: CMV.
LacZPurified$500
AA023500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing LacZ.
Serotype: 2.   Promoter: CMV.
LacZPurified$650
AA028500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing LacZ.
Serotype: 2.   Promoter: CMV.
LacZStandard$300
AA0291ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing LacZ.
Serotype: 2.   Promoter: CMV.
LacZStandard$400
AA032100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: 2.   Promoter: CMV.
CREPurified$500
AA033500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: 2.   Promoter: CMV.
CREPurified$650
AA038500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: 2.   Promoter: CMV.
CREStandard$300
AA0391ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: 2.   Promoter: CMV.
CREStandard$400
AA062100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing mCherry.
Serotype: 2.   Promoter: CMV.
mCherryPurified$500
AA063500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing mCherry.
Serotype: 2.   Promoter: CMV.
mCherryPurified$650
AA068500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing mCherry.
Serotype: 2.   Promoter: CMV.
mCherryStandard$300
AA0691ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing mCherry.
Serotype: 2.   Promoter: CMV.
mCherryStandard$400
AA072100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: 2.   Promoter: CMV.
FLucPurified$500
AA073500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: 2.   Promoter: CMV.
FLucPurified$650
AA078500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: 2.   Promoter: CMV.
FLucStandard$300
AA0791ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: 2.   Promoter: CMV.
FLucStandard$400
AA092100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: 2.   Promoter: CMV.
GLucPurified$500
AA093500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: 2.   Promoter: CMV.
GLucPurified$650
AA098500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: 2.   Promoter: CMV.
GLucStandard$300
AA0991ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: 2.   Promoter: CMV.
GLucStandard$400
AA202100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing eGFP.
Serotype: DJ.   Promoter: CMV.
eGFPPurified$500
AA203500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing eGFP.
Serotype: DJ.   Promoter: CMV.
eGFPPurified$650
AA208500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing eGFP.
Serotype: DJ.   Promoter: CMV.
eGFPStandard$300
AA2091ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing eGFP.
Serotype: DJ.   Promoter: CMV.
eGFPStandard$400
AA212100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: DJ.   Promoter: CMV.
IRES-hrGFPPurified$500
AA213500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: DJ.   Promoter: CMV.
IRES-hrGFPPurified$650
AA218500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: DJ.   Promoter: CMV.
IRES-hrGFPStandard$300
AA2191ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: DJ.   Promoter: CMV.
IRES-hrGFPStandard$400
AA222100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing LacZ.
Serotype: DJ.   Promoter: CMV.
LacZPurified$500
AA223500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing LacZ.
Serotype: DJ.   Promoter: CMV.
LacZPurified$650
AA228500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing LacZ.
Serotype: DJ.   Promoter: CMV.
LacZStandard$300
AA2291ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing LacZ.
Serotype: DJ.   Promoter: CMV.
LacZStandard$400
AA232100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: DJ.   Promoter: CMV.
CREPurified$500
AA233500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: DJ.   Promoter: CMV.
CREPurified$650
AA238500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: DJ.   Promoter: CMV.
CREStandard$300
AA2391ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: DJ.   Promoter: CMV.
CREStandard$400
AA262100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing mCherry.
Serotype: DJ.   Promoter: CMV.
mCherryPurified$500
AA263500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing mCherry.
Serotype: DJ.   Promoter: CMV.
mCherryPurified$650
AA268500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing mCherry.
Serotype: DJ.   Promoter: CMV.
mCherryStandard$300
AA2691ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing mCherry.
Serotype: DJ.   Promoter: CMV.
mCherryStandard$400
AA272100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: DJ.   Promoter: CMV.
FLucPurified$500
AA273500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: DJ.   Promoter: CMV.
FLucPurified$650
AA278500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: DJ.   Promoter: CMV.
FLucStandard$300
AA2791ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: DJ.   Promoter: CMV.
FLucStandard$400
AA292100µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: DJ.   Promoter: CMV.
GLucPurified$500
AA293500µl
≥ 5x10^12 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: DJ.   Promoter: CMV.
GLucPurified$650
AA298500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: DJ.   Promoter: CMV.
GLucStandard$300
AA2991ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: DJ.   Promoter: CMV.
GLucStandard$400
AA408500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing eGFP.
Serotype: DJ/8.   Promoter: CMV.
eGFPStandard$360
AA4091ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing eGFP.
Serotype: DJ/8.   Promoter: CMV.
eGFPStandard$480
AA418500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: DJ/8.   Promoter: CMV.
IRES-hrGFPStandard$360
AA4191ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing IRES-hrGFP.
Serotype: DJ/8.   Promoter: CMV.
IRES-hrGFPStandard$480
AA428500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing LacZ.
Serotype: DJ/8.   Promoter: CMV.
LacZStandard$360
AA4291ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing LacZ.
Serotype: DJ/8.   Promoter: CMV.
LacZStandard$480
AA438500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: DJ/8.   Promoter: CMV.
CREStandard$360
AA4391ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing CRE recombinase.
Serotype: DJ/8.   Promoter: CMV.
CREStandard$480
AA468500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing mCherry.
Serotype: DJ/8.   Promoter: CMV.
mCherryStandard$360
AA4691ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing mCherry.
Serotype: DJ/8.   Promoter: CMV.
mCherryStandard$480
AA478500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: DJ/8.   Promoter: CMV.
FLucStandard$360
AA4791ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing Firefly luciferase.
Serotype: DJ/8.   Promoter: CMV.
FLucStandard$480
AA498500µl
≥ 1x10^11 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: DJ/8.   Promoter: CMV.
GLucStandard$360
AA4991ml
≥ 1x10^11 GC/ml
Premade AAV particles expressing Gaussia luciferase.
Serotype: DJ/8.   Promoter: CMV.
GLucStandard$480

 

 

 

Serotypes & Vectors

Order custom AAV particles

Custom AAV particles production services are currently available for ORF expression. GeneCopoeia' s scientific expertise can deliver AAV particles with titers starting from 10^11 GC/mL or higher depending upon the request.

For all AAV particles not covered in the premade particles collection, request a custom quote here.

 

AAV serotypes available

GeneCopoeia provides AAV particles for all the commonly-occurring AAV serotypes. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different tissues. 

Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to compare the essential differences between different serotypes.

Serotype Primary Target Tissues Description
AAV-1 Muscle Best for cardiac muscle, skeletal muscle, neuronal and glial tissue.
AAV-2 Muscle, Liver, Retina Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain.
AAV-3 Megakaryocytes Best for megakaryocytes, muscle, liver, lung, and retina.
AAV-4 Retina Best for neurons, muscle, brain, and retina.
AAV-5 Lung Best for lung, neurons, synovial joint, retina, and pancreas.
AAV-6 Muscle, Lung Best for lung, liver, and heart.
AAV-7 Muscle, Retina, Neurons Best for muscle, neurons, and liver.
AAV-8 Liver Best for muscle, brain, liver, and retina.
AAV-9 Various Best for muscle, heart, liver, lung, and brain.
AAV-10 Pleura, CNS Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, NCS and liver.
AAV-DJ Various A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro.
AAV-DJ/8 Various A variant of AAV-DJ with a heparin binding domain (HBD) mutation, which permits infection of liver as well as other tissues in vivo.

 

 

Vector choices

GeneCopoeia’s AAV vector collection enables efficient gene expression in a broad range of cell types. For insert lengths up to 3kb.

Vector name Promoter Promoter description Reporter gene
pEZ-AV01 CMV Strong promoter, broad scope of application. N/A
pEZ-AV02 EF-1α Broad scope of application. Better choice than CMV for in vivo experiments. N/A
pEZ-AV04 CAG Ubiquitous. Strong promoter for in vivo use. N/A

* More vectors are coming soon!

 

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