GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for inserting genes into a broad range of cell types with high efficiency and enhanced safety. GeneCopoeia’s optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made particle options are available in 3 different serotypes, including fluorescent reporters (Figure 1), or you can have us produce custom AAV particles for you with genes up to 3 kb in length. Particle preparations are available in either standard (for in vitro use only; Figure 1) or purified (suitable for in vivo use; Figure 2) formats.
High titers. Titer of purified particles can be up to 10^14 GC/ml (genome copies/ml)
Versatile. Usable in a broad range of host cell types
Low toxicity. Does not integrate into the host genome.
Low immunogenicity. Minimal host immune response.
Safe. Not associated with any human disease
GeneCopoeia's AAVPrime™ standard AAV particles expressing fluorecent reporters
Figure 1. HT1080 cells in 24-well plates were transduced with 0.5 µL of AAV standard format particles expressing either eGFP (A), RFP (B), or mCherry (C)). Cells were visualized with a fluorescence microscope (Exposure time: 400 ms).
Figure 2. HT1080 cells in 24-well plates were transducted with the indicated amounts of AAV purified particles expressing eGFP. Cells were visualized with a fluorescence microscope (Exposure time: 400 ms).
Order premade AAV particles
By default, all options for AAV particle serotype, purity level (standard or purified) and volume are listed in the table below. Use the drop-down menus to narrow the number of options displayed.
Order custom AAV particles
Custom AAV particles production services are currently available for ORF expression. GeneCopoeia' s scientific expertise can deliver AAV particles with titers starting from 10^11 GC/mL or higher depending upon the request.
GeneCopoeia provides AAV particles for the most commonly-occurring AAV serotypes. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different kinds of tissue.
Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to learn the essential differences between the different serotypes.
Main Target Tissues
AAV – 1
Efficient for muscle, heart, skeletal muscle, nervous and gial tissue directed genetransfer.
AAV – 2
Muscle, Liver, Retina
Most early and classical serotype that is applied in wide range. Efficient for nervous centralis, muscle, liver, brain, retina directed genetransfer.
AAV – 3
Efficient for muscle, liver, lung, retina directed genetransfer.
AAV – 4
Efficient for nervous centralis, muscle, brain, retina directed genetransfer.