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Recombination Cloning Technology

Recombination cloning technology is based on the site specific recombination reaction of phage lambda, and is the same principle as Gateway® technology, an efficient biochemical process that conserves genetic information (see Figure 1). The transmission of an ORF from EZShuttle Gateway® PLUS ORF Clones to a pEZ™ or Gateway® Destination Vector is a one step simple and rapid operation (60 minutes at room temperature), which saves a significant amount of time and effort from the tedious, multiple step, sub-cloning process involving enzyme digestions, ligations, gel electrophoresis and purification (see Figure 2). Furthermore, GeneCopoeia provides a family of pEZ™ vectors that contain different promoters and fusion-tags that meet the distinct requirements for production and purification of desired recombinant proteins in several expression hosts.

 

Figure 1. Site specific recombination reaction of phage lambda, Click on the image to see enlarged image Figure 2. One step transmission of an ORF from EZShuttle™ Gateway PLUS ORF clones to a pEZ™ or Gateway destination vector. Click on the image to see enlarged image

 

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