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COVID-19 Coronavirus Plasmid Clones

by | Jun 24, 2020 | Products

General information

GeneCopoeia is the industry leader in expression-ready ORF cDNA clones, and we also carry reporter plasmid clones carrying gene promoters and 3′ UTR microRNA (miRNA) binding site targets. OmicsLink™ SARS-CoV-2-related ORF cDNA plasmid clones can be constructed in any of our more than 250 ready-to-go vector types expressing both SARS-CoV-2 viral proteins as well as host proteins, which you can select using the drop-down menus below.

OmicsLink™ SARS-CoV-2-related ORF cDNA plasmid clones are part of GeneCopoeia’s COVID-19 Coronavirus Research Tools products.

Note: SARS-CoV-2-related ORF cDNA plasmid clones are for research purposes only. They are not intended for diagnostic or treatment usage in humans.

We also have OmicsLink™ ORF cDNA plasmid clones for the related virus SARS-CoV-1, which caused the 2002-2004 SARS outbreak.

COVID-19 ORF cDNA plasmid clones-viral Spike proteins

Order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral Spike proteins

To order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral Spike proteins in one of our more than 250 non-viral and lentiviral vector types, select the product ID from the table below and contact inquiry@genecopoeia.com.

To view the Spike mutations in each Spike variant, refer to the table “Spike variant mutational profiles” below.

Product ID Protein accession Symbol Alias Spike genotype Description
NV219 YP_009724390.1 S GU280_gp02 WT surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene (codon optimized)
NV236 YP_009724390.1 S GU280_gp02 D614G surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G mutation (codon optimized)
NV237 YP_009724390.1 S GU280_gp02 N439K surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with N439K mutation (codon optimized)
NV238 YP_009724390.1 S GU280_gp02 D614G, N439K surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with N439K, D614G mutations (codon optimized)
NV239 YP_009724390.1 S GU280_gp02 D614G, E501Y surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, E501Y mutations (codon optimized)
NV240 YP_009724390.1 S GU280_gp02 D614G, P681H surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, P681H mutations (codon optimized)
NV241 YP_009724390.1 S GU280_gp02 D614G, Hv69-90deletion surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, Hv69-90deletion mutations (codon optimized)
NV242 YP_009724390.1 S GU280_gp02   D614G, U.K. B.1.1.7   surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, U.K. B.1.1.7 mutations (codon optimized)
NV244 YP_009724390.1 S GU280_gp02   D614G, South Africa B.1.351   surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, South Africa B.1.351 mutations (codon optimized)
NV245 YP_009724390.1 S GU280_gp02   D614G, Brazil P.1   surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, Brazil P.1 mutations (codon optimized)
NV247 YP_009724390.1 S GU280_gp02 California B.1.427/B.1.429 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with California B.1.427/B.1.429 mutations (codon optimized)
NV248 YP_009724390.1 S GU280_gp02 India B.1.617 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with India B.1.617 mutations (codon optimized)
NV220 YP_009724390.1 S1 GU280_gp02 WT surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide (codon optimized)
NV221 YP_009724390.1 S2 GU280_gp02 WT surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)
NV222 YP_009724390.1 RBD GU280_gp02 WT Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)

Spike variant mutational profiles

At the time of product introduction, GeneCopoeia’s R&D team used the GISAID database to determine the most frequently-occurring mutations in the Spike proteins from each variant to incorporate into our Spike-expressing ORF clones. These mutational profiles are indicated below. Note that each variant can have different sub-lineages, which could contain a slightly different repertoire of mutations from the variants that we have made available. If you would like SARS-CoV-2 Spike-expressing ORF clones with a different set of mutations than we have currently available. please contact inquiry@genecopoeia.com for a custom project.

                 
SARS-CoV-2 variant Spike mutations
Wild type (product IDs NV219, NV220, NV221, NV222) None
D614G (product ID NV236) D614G
N439K (product ID NV237) N439K
N439K & D614G (product ID NV238) N439K, D614G
N439K & N501Y (product ID NV239) N439K, N501Y
D614G & P681H (product ID NV240) D614G, P681H
D614G & Hv69-90deletion (product ID NV241) Δ69-70, D614G
B.1.1.7 (first identified in the U.K.; product ID NV242) Δ69-70, Δ144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
B.1.351 (first identified in South Africa; product ID NV244) L18F, D80A, D215G，Δ242-244, K417N, E484K, N501Y, D614G, A701V
P.1 (first identified in Brazil; product ID NV245) L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
 

COVID-19 ORF cDNA plasmid clones-viral non-Spike proteins

Order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral proteins

To order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral proteins in one of our more than 250 non-viral and lentiviral vector types, select the product ID from the table below and contact inquiry@genecopoeia.com.
Product ID Protein accession Symbol Alias Description
NV201 (please inquire) YP_009724389.1 orf1ab GU280_gp01 orf1ab polyprotein [Severe acute respiratory syndrome coronavirus 2}, full-length gene
NV202 YP_009725297.1 orf1ab GU280_gp01 leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV203 YP_009725298.1 orf1ab GU280_gp01 nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV204 YP_009725299.1 orf1ab GU280_gp01 nsp3n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV205 YP_009725300.1 orf1ab GU280_gp01 nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV206 YP_009725301.1 orf1ab GU280_gp01 3C-like proteinase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV207 YP_009725302.1 orf1ab GU280_gp01 nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV208 YP_009725303.1 orf1ab GU280_gp01 nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV209 YP_009725304.1 orf1ab GU280_gp01 nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV210 YP_009725305.1 orf1ab GU280_gp01 nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV211 YP_009725306.1 orf1ab GU280_gp01 nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV212 YP_009725307.1 orf1ab GU280_gp01 RNA-dependent RNA polymerase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV213 YP_009725308.1 orf1ab GU280_gp01 helicase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV214 YP_009725309.1 orf1ab GU280_gp01 3′-to-5′ exonuclease [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV215 YP_009725310.1 orf1ab GU280_gp01 endoRNAse [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV216 YP_009725311.1 orf1ab GU280_gp01 2′-O-ribose methyltransferase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV217 (please inquire) YP_009725295.1 orf1a GU280_gp01 orf1a polyprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV218 YP_009725312.1 orf1a GU280_gp01 produced by pp1a only [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV223 YP_009724391.1 ORF3a GU280_gp03 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV224 YP_009724392.1 E GU280_gp04 envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV225 YP_009724393.1 M GU280_gp05 membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV226 YP_009724394.1 ORF6 GU280_gp06 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV227 YP_009724395.1 ORF7a GU280_gp07 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV228 YP_009725318.1 ORF7b   GU280_gp08   ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV229 YP_009724396.1 ORF8 GU280_gp09 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV230 YP_009724397.2 N GU280_gp10 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV231 YP_009725255.1 ORF10 GU280_gp11 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
   

*Note: Mature peptides are produced as a result of proteolytic processing of larger precursor proteins. To enable expression of these proteins in cells, an ATG has been added to the 5′ end of the ORF sequence.

  For more information or to request a quote, please contact inquiry@genecopoeia.com.

COVID-19 ORF cDNA plasmid clones-host proteins

ACE2

You can purchase ORF cDNA clones expressing human host proteins that SARS-CoV-2 interacts with, including ACE2, which encodes the receptor for SARS-CoV-2 on respiratory epithelial cells, in more than 250 non-viral and lentiviral vector types. Either view all standard clones using the buttons below, or choose human clones in several popular vector types in the table. If you don’t see what you need here, please contact inquiry@genecopoeia.com.

View all ACE2 ORF cDNA clones for human, mouse, and rat

Order human ACE2 ORF cDNA clones in popular vector types

Clones in yellow shading are available for next-day shipping in bacterial stock format.**
 
Buy Catalog# Vector Type Promoter Tag / Reporter / Other Features Mammalian Selection Markers Vector Name List Price Discount price
ORF cDNA plasmid clones expressing human ACE2
EX-U1285-M02-B (Bacterial stock) Mammalian CMV Untagged Neomycin pReceiver-M02 $846 $469
EX-U1285-M02-10 (10 ug purified plasmid) Mammalian CMV Untagged Neomycin pReceiver-M02 $896 $519
EX-U1285-M98-B (Bacterial stock) Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $846 $469
EX-U1285-M98-10 (10 ug purified plasmid) Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $896 $519
EX-U1285-M35 (10 ug purified plasmid) Mammalian CMV C-Flag Neomycin pReceiver-M35 $846 $846
EX-U1285-Lv105-B (Bacterial stock) Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $896 $523
EX-U1285-Lv105-10 (10 ug purified plasmid) Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $946 $573
EX-U1285-Lv242-B (Bacterial stock) Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $946 $523
EX-U1285-Lv242-10 (10 ug purified plasmid) Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $996 $573
GC-U1285-B (Bacterial stock) Gateway PLUS shuttle — Untagged — pShuttle™ $846 $523
GC-U1285-10 (10 ug purified plasmid) Gateway PLUS shuttle — Untagged — pShuttle™ $896 $573
   
 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

** Next-day shipping is available for NextDay ORF clones in bacterial stock format. Lead times for NextDay ORF clones in purified plasmid is ~3-5 days. Orders for NextDay ORF clones must be received by 12PM EST Mon-Thurs in order to be processed and shipped the next day.
TMPRSS2

You can purchase ORF cDNA clones expressing human host proteins that SARS-CoV-2 interacts with, including TMPRSS2, a serine protease required for S protein priming, in more than 250 non-viral and lentiviral vector types. Either view all standard clones using the buttons below, or choose human clones in several popular vector types in the table. If you don’t see what you need here, please contact inquiry@genecopoeia.com.

View all TMPRSS2 ORF cDNA clones for human, mouse, and rat

Order human TMPRSS2 ORF cDNA clones in popular vector types

Clones in yellow shading are available for next-day shipping in bacterial stock format.**
 
Buy Catalog# Vector Type Promoter Tag / Reporter / Other Features Mammalian Selection Markers Vector Name List Price Discount price
ORF cDNA plasmid clones expressing human TMPRSS2
EX-Z7591-M02-B (Bacterial stock) Mammalian CMV Untagged Neomycin pReceiver-M02 $432 $324
EX-Z7591-M02-10 (10 ug purified plasmid) Mammalian CMV Untagged Neomycin pReceiver-M02 $482 $374
EX-Z7591-M98-B (Bacterial stock) Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $432 $324
EX-Z7591-M98-10 (10 ug purified plasmid) Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $482 $374
EX-Z7591-M35 (10 ug purified plasmid) Mammalian CMV C-Flag Neomycin pReceiver-M35 $482 $374
EX-Z7591-Lv105-B (Bacterial stock) Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $482 $379
EX-Z7591-Lv105-10 (10 ug purified plasmid) Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $532 $429
EX-Z7591-Lv242-B (Bacterial stock) Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $532 $379
EX-Z7591-Lv242-10 (10 ug purified plasmid) Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $582 $429
GC-Z7591-B (Bacterial stock) Gateway PLUS shuttle — Untagged — pShuttle™ $432 $379
GC-Z7591-10 (10 ug purified plasmid) Gateway PLUS shuttle — Untagged — pShuttle™ $482 $429
   

For more information or to request a quote, please contact inquiry@genecopoeia.com.

** Next-day shipping is available for NextDay ORF clones in bacterial stock format. Lead times for NextDay ORF clones in purified plasmid is ~3-5 days. Orders for NextDay ORF clones must be received by 12PM EST Mon-Thurs in order to be processed and shipped the next day.
Cyclophilin A

The cyclophilins are a family of proline isomerases that function to aid in protein folding. Cyclophilins bind to the immunosuppressive drug cyclosporin A (CsA) and help mediate CsA’s pharmacological function. While there is no published evidence yet that cyclophilins are involved in the pathogenesis of SARS-CoV-2, several groups have shown that CsA can inhibit several viruses, including the virus that caused the 2002-2004 SARS pandemic, SARS-CoV, in a cyclophilin A (CypA)-dependent fashion (reviewed in Tanaka, Y., et al., 2013. Viruses 5, 1,250). Further, CypA interacts with SARS-CoV Non-structural protein 1 (Nsp1) as demonstrated in a yeast 2-hybrid screen (Pfefferle, E., et al.. 2011; PLOS Pathogens doi:  doi:10.1371/journal.ppat.1002331). SARS-CoV is highly similar to SARS-CoV-2, and, in fact, both viruses use ACE2 as their cellular receptor. Therefore, it is reasonable to include CypA in investigations of a potential role for CypA in SARS-CoV-2 pathogenesis.

GeneCopoeia provides non-viral and lentiviral ORF cDNA clones in 250+ vector types for CYPA in human, mouse, and rat.

 

View all human cyclophilin A ORF cDNA clones

Humans have two known isoforms of cyclophilin A (PPIA). You can order human cyclophilin ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below

Product ID Accession Symbol Alias Description
F0323 NM_021130.4 PPIA CYPA, CYPH, HEL-S-69p Homo sapiens peptidylprolyl isomerase A (PPIA), transcript variant 1, mRNA.
T2782 NM_001300981.1 PPIA CYPA, CYPH, HEL-S-69p Homo sapiens peptidylprolyl isomerase A (PPIA), transcript variant 2, mRNA.

View all mouse M. musculus cyclophilin A ORF cDNA clones

Mouse M. musculus has only one known isoform of cyclophilin A (PPIA). You can order mouse cyclophilin A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

Product ID Accession Symbol Alias Description
Mm17577 NM_008907.1 Ppia 2700098C05, Cphn, CyP-18, CypA Mus musculus peptidylprolyl isomerase A (Ppia), mRNA.

View all rat R. norvegicus cyclophilin A ORF cDNA clones

Rat R. norvegicus has only one known isoform of cyclophilin A (PPIA). You can order rat cyclophilin A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

Product ID Accession Symbol Alias Description
Rn24256 NM_017101.1 Ppia CYCA, CyP-A Rattus norvegicus peptidylprolyl isomerase A (Ppia), mRNA.
For more information or to request a quote, please contact inquiry@genecopoeia.com.
FKBP1A

FK506 binding proteins (FKBP) are, like cyclophilins, proline isomerases that bind to another immunosuppressive drug, FK506 and mediate its pharmacology. While there is no published evidence yet that FKBPs are involved in the pathogenesis of SARS-CoV-2, several groups have demonstrated that FKBP1A is important for assembly of the virus that caused the 2002-2004 SARS pandemic, SARS-CoV (Pfefferle, S., et al., 2011; PLOS Pathogens doi: 10.1371/journal.ppat.1002331). SARS-CoV is highly similar to SARS-CoV-2, and, in fact, both viruses use ACE2 as their cellular receptor. Therefore, it is reasonable to include FKBP1A in investigations of a potential role for FKBP1A in SARS-CoV-2 pathogenesis.

GeneCopoeia provides non-viral and lentiviral ORF cDNA clones in 250+ vector types for FKBP1A in human, mouse, and rat.

 

View all human FKBP1A ORF cDNA clones

Humans have two known isoforms of FKBP1A. You can order human FKBP1A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below

Product ID Accession Symbol Alias Description
R0062 NM_000801.4 FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Homo sapiens FK506 binding protein 1A (FKBP1A), transcript variant 1, mRNA.
Z8544 NM_001199786.1 FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Homo sapiens FK506 binding protein 1A (FKBP1A), transcript variant 3, mRNA.

View all mouse M. musculus FKBP1A ORF cDNA clones

Mouse M. musculus has four known isoforms of FKBP1A. You can order mouse FKBP1A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

Product ID Accession Symbol Alias Description
Mm02519 NM_008019.2 Fkbp1a 12kDa, FKBP12, FKBP12-T1, FKBP12-T2, Fkbp, Fkbp1, mFKBP1, mFKBP12 Mus musculus FK506 binding protein 1a (Fkbp1a), mRNA.
Mm38146 NM_001302078.1 Fkbp1a FKBP12, Fkbp, Fkbp1 Mus musculus FK506 binding protein 1a (Fkbp1a), transcript variant 4, mRNA.
Mm38147 NM_001302079.1 Fkbp1a FKBP12, Fkbp, Fkbp1 Mus musculus FK506 binding protein 1a (Fkbp1a), transcript variant 5, mRNA.
Mm38985 NM_001355078.1 Fkbp1a FKBP12, Fkbp, Fkbp1 Mus musculus FK506 binding protein 1a (Fkbp1a), transcript variant 8, mRNA.

View all rat R. norvegicus FKBP1A ORF cDNA clones

Rat R. norvegicus has only one known isoform of FKBP1A. You can order rat FKBP1A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

Product ID Accession Symbol Alias Description
Rn24112 NM_013102.3 Fkbp1a FKBP12, Fkbp2 Rattus norvegicus FKBP prolyl isomerase 1A (Fkbp1a), mRNA.
For more information or to request a quote, please contact inquiry@genecopoeia.com.
SARS-CoV-2 human interacting protein ORF cDNA plasmid clones

A recent publication in Nature (Gordon, et al., 2020) described the results of an affinity purification-mass spectrometry (AP-MS) screen that identified 332 human proteins that physically interact with 26 SARS-CoV-2 viral proteins. While it is unlikely that all 332 interactions are physiologically relevant, Gordon, et al. demonstrated that 66 of these human host proteins are potential targets of known drugs used for other purposes, and that a subset of these drugs inhibit viral replication in vitro.

You can order all the clones encoding these human interacting proteins, either individually or as a complete library, in any of our 250+ non-viral or lentiviral vector types. To order complete libraries, please visit our ORF cDNA library page.

Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentivirus

by | Jun 17, 2020 | Products

GeneCopoeia’s Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products produce a specially-packaged version of standard lentivirus, whereby the vesicular stomatitis G (VSV-G) envelope glycoprotein is replaced by the Spike (S) protein from SARS-CoV-2, the virus responsible for the COVID-19 pandemic. These particles, also known as “pseudoviruses”, are typically used to infect cultured cells expressing ACE2, the receptor for SARS-CoV-2 on multiple  tissue types (Figure 1).

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus and our ACE2- and TMPRSS2-expressing cell lines are great for several applications in a safe environment without the use of pathogenic SARS-CoV-2 viruses, such as:

  • Assessment of the efficacy of vaccines against SARS-CoV-2 virus variants
  • Studying the efficacy and mechanism of neutralizing antibodies against SARS-CoV-2 virus variants
  • Development of antiviral therapeutic agents
  • Studying the mechanism of virus-receptor interaction

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products are part of GeneCopoeia’s COVID-19 Coronavirus Research Tools products. Note: Spike-pseudotyped lentiviruses are for research purposes only. They are not intended for diagnostic or treatment usage in humans.

Ordering information

Ordering information

Use the drop-down menus below to view products for lentivirus packaged with SARS-CoV-2 Spike protein.

For more information, please contact inquiry@genecopoeia.com.

SARS-CoV-2 Spike protein-pseudotyped lentiviral particles

GeneCopoeia’s Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentivirus is available as either premade, in vitro grade or custom-produced in vivo grade particles. Pseudoviral particles expressing Spike proteins from the original (“WT”) version as well as other variants, such as B.1.1.7 (first identified in the U.K.) and B.1.351 (first identified in South Africa) are available.

Advantages

  • Accurate functional titers.  Functional titers are measured by FACS sorting of ACE2-expressing HEK293T cells infected with GFP-expressing particles.
  • Complete system. Premade, in vitro-grade purified particles can be used with GeneCopoeia’s ACE2-expressing HEK293T cells as well as other cells permissive to SARS-CoV-2 infection. Kits for do-it-yourself packaging of Spike-pseudotyped lentivirus also available.
  • Quantitative measurements. Expression of GFP and luciferase enables quantitative determination of infection efficiency that is more accurate than traditional plaque reduction assays.
  • Safe. Study SARS-CoV-2 Spike-mediated interactions with cells in the absence of pathogenic virus.

For more information, please contact inquiry@genecopoeia.com.

Lentifect™ lentiviral particles for SARS-CoV-2 coronavirus research (in vitro grade)

NOTE: Use the filters to select/de-select options for the type of envelope protein and which SARS-CoV-2 Spike variant you want expressed on the surface of your SARS-CoV-2 Spike pseudotyped lentivirus.

To view the Spike mutations in each pseudovirus variant, refer to the table “Spike variant mutational profiles” below.

Envelope protein :   Spike variant
Buy Catalog Product Description Spike variant4 Envelope protein Reporter / marker Vector Price
SP101-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1 × 105TU/ml1 or > 1× 107RLU/ml* lentiviral particles ready for transduction, 100 µl WT Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP103-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length D614G Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl D614G Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP104-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length N439K Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×105TU/ml1 or > 1×107RLU/ml* lentiviral particles ready for transduction, 100 µl N439K Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP105-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (D614G & N439K) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl D614G & N439K Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP106-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Alpha) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×105TU/ml1 or > 1×107RLU/ml* lentiviral particles ready for transduction, 100 µl Alpha Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP107-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Beta) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Beta Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP108-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Gamma) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Gamma Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP109-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (India B.1.617.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl India B.1.617 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP110-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (California B.1.427/B.1.429) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl California B.1.427/B.1.429 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP111-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Delta) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Delta Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP112-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Lambda C.37) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×108TU/ml1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Lambda C.37 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP113-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Mu B.1.621) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×107TU/ml1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Mu B.1.621 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP115-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP116-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BA.2) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.2 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP117-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BA.2.12.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.2.12.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP118-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BA.4/BA.5/BA.5.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.4/BA.5/BA.5.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP119-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BA.4.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.4.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP120-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BF.7) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BF.7 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP121-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BQ.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BQ.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP122-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron BQ.1.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BQ.1.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP123-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron XBB) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron XBB Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP124-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron XBB.1) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron XBB.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP125-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Omicron XBB.1.5) Spike-Pseudotyped Lentiviral Particles (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron XBB.1.5 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $892
SP001-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×106TU/ml1 or >1×108RLU/ml* lentiviral particles ready for transduction, 100 µl WT Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP003-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 D614G Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 5×106TU/ml1 or >5×108RLU/ml* lentiviral particles ready for transduction, 100 µl D614G Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP004-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 N439K Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×106TU/ml1 or > 1×108RLU/ml* lentiviral particles ready for transduction, 100 µl N439K Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP005-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (D614G & N439K) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 5×106TU/ml1 or > 5×108RLU/ml* lentiviral particles ready for transduction, 100 µl D614G & N439K Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP006-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Alpha) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 5×106TU/ml1 or > 5×108RLU/ml* lentiviral particles ready for transduction, 100 µl Alpha Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP007-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Beta) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 5×106TU/ml1 or > 5×108RLU/ml* lentiviral particles ready for transduction, 100 µl Beta Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP009-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (India B.1.617) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×105TU/ml1 or > 1×107RLU/ml* lentiviral particles ready for transduction, 100 µl India B.1.617 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP010-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (California B.1.427/B.1.429) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×105TU/ml1 or > 1×107RLU/ml* lentiviral particles ready for transduction, 100 µl California B.1.427/B.1.429 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP011-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Delta) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 5×106TU/ml1 or >5×108RLU/ml* lentiviral particles ready for transduction, 100 µl Delta Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP012-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Lambda C.37) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×105TU/ml 1 or > 1×107RLU/ml* lentiviral particles ready for transduction, 100 µl Lambda C.37 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP013-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Mu B.1.621) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×105TU/ml 1 or > 1×107RLU/ml* lentiviral particles ready for transduction, 100 µl Mu B.1.621 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP015-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP016-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BA.2) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.2 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP017-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BA.2.12.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.2.12.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP018-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BA.4/BA.5/BA.5.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.4/BA.5/BA.5.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP019-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BA.4.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BA.4.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP020-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BF.7) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BF.7 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP021-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BQ.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BQ.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP022-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron BQ.1.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron BQ.1.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP023-100

Backordered

 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron XBB) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron XBB Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP024-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron XBB.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron XBB.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
SP025-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Omicron XBB.1.5) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl × 2 vials) > 1×107TU/ml 1 or > 1×109RLU/ml* lentiviral particles ready for transduction, 100 µl Omicron XBB.1.5 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $892
LP464-025 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl pReceiver-Lv201,/wp-content/uploads/oldpdfs/tech/omicslink/pReceiver-Lv201.pdf standard VSV-G glycoprotein eGFP/Puro, HLUC pReceiver-Lv201 $319
LP464-100 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 4 vials) > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl pReceiver-Lv201,/wp-content/uploads/oldpdfs/tech/omicslink/pReceiver-Lv201.pdf standard VSV-G glycoprotein eGFP/Puro, HLUC pReceiver-Lv201 $784
SP401-025 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 1 vial) > 1×1010 copies/ml3 lentiviral particles ready for transduction, 25 µl N/A None eGFP/Puro, HLUC pReceiver-Lv201 $319
SP401-100 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 4 vial) > 1×1010 copies/ml3 lentiviral particles ready for transduction, 100 µl N/A None eGFP/Puro, HLUC pReceiver-Lv201 $784

 

1 The functional titers of SARS-CoV-2 Spike pseudotyped lentivirus are determined by FACS sorting of GFP positive ACE2-expressing ACE2/HEK293T cells (GeneCopoeia Cat.No. SL222) and by luciferase analysis (GeneCopoeia Cat.No. LF007).

2Titers of standard VSV-G pseudotyped lentivirus are determined by RT-qPCR to determine physical titer (copies/ml) and extrapolated to functional titer (TU/ml).

3The titer of the lentiviral particles without envelope protein is determined by RT-qPCR. 

4SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

*Truncated SARS-CoV-2 Spike proteins carry a deletion of the 19 amino acid C-terminal endoplasmic reticulum retention signal.

Spike variant mutational profiles

At the time of product introduction, GeneCopoeia’s R&D team used the GISAID database to determine the most frequently-occurring mutations in the Spike proteins from each variant to incorporate into our pseudovirus particles. These mutational profiles are indicated below. Note that each variant can have different sub-lineages, which could contain a slightly different repertoire of mutations from the variants that we have made available. If you would like SARS-CoV-2 Spike-pseudotyped lentivirus expressing a different set of mutations than we have currently available. please contact inquiry@genecopoeia.com for a custom project.

SARS-CoV-2 variant Spike mutations
Wild type (catalog numbers SP001-100, SP101-100) None
D614G (catalog numbers SP003-100, SP103-100) D614G
N439K (catalog numbers SP004-100, SP104-100) N439K
N439K & D614G (catalog numbers SP005-100, SP105-100) N439K, D614G
B.1.1.7 (first identified in the U.K.; catalog numbers SP006-100, SP106-100) Δ69-70, Δ144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
B.1.351 (first identified in South Africa; catalog numbers SP007-100, SP107-100) L18F, D80A, D215G，Δ242-244, K417N, E484K, N501Y, D614G, A701V
P.1 (first identified in Brazil; catalog numbers SP008-100, SP108-100) L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
B.1.617.1 (first identified in India; catalog numbers SP009-100, SP109-100) G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H
B.1.427/B.1.429 (first identified in California, USA; catalog numbers SP010-100, SP110-100) S13I, W152C, L452R, D614G
B.1.617.2 (first identified in India; catalog numbers SP011-100, SP111-100) T19R, G142D, 156del, 157del, R158G, L452R, T478K, D614G, P681R, D950N
Lambda C.37 (first identified in Peru; catalog numbers SP012-100, SP112-100) G75V, T76I, del246/253, L452Q, F490S, D614G, T859N
Mu B.1.621 (first identified in Columbia; catalog numbers SP013-100, SP113-100) T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N
Omicron B.1.1.529 (first identified in South Africa; catalog numbers SP015-100, SP115-100) A67V, Del 69-70HV, T95I, G142D, Del 143-145VYY, N211I, del 212L, insert215-217 EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F
Omicron BA.2 (first identified in South Africa; catalog numbers SP016-100, SP116-100) T19I, L24S, del25/27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BA.2.12.1 (first identified in South Africa; catalog numbers SP017-100, SP117-100) T19I, L24S, del25/27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, Q954H, N969K
Omicron BA.4/BA.5/BA.5.1 (first identified in South Africa; catalog numbers SP018-100, SP118-100) T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BA.4.1 (first identified in South Africa; catalog numbers SP019-100, SP119-100) V3G, T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BF.7 (first identified in South Africa; catalog numbers SP020-100, SP120-100) T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BQ.1 (first identified in South Africa; catalog numbers SP021-100, SP121-100) T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BQ.1.1 (first identified in South Africa; catalog numbers SP022-100, SP122-100) T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron XBB (first identified in South Africa; catalog numbers SP023-100, SP123-100) T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron XBB.1 (first identified in South Africa; catalog numbers SP024-100, SP124-100) T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron XBB.1.5 (first identified in South Africa; catalog numbers SP025-100, SP125-100) T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K

 

COVID-19 Coronavirus Receptor Stable Cell Lines

Need a cell line to infect with your SARS-CoV-2 Spike pseudotyped lentiviral particles? GeneCopoeia has produced HEK293T cell lines expressing human ACE2 alone, or ACE2 in combination with TMPRSS2, a protease that promotes Spike-ACE2 receptor interaction. These cells are amenable to infection by viruses expressing SARS-CoV-2 Spike protein in their envelopes. For more detailed information regarding these cell lines, please visit our COVID-19 Coronavirus Receptor Stable Cell Lines page.

You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

In addition to the products below, custom stable cell lines are also available. For more information, please contact inquiry@genecopoeia.com.

Buy Catalog # Cell line Description Cell type Selection marker Price
SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $4321
SL222 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells Embryonic kidney

mCherry/

Puromycin,

Hygromycin

 $5186

 

  Warranty statement and limited use license   MSDS
 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

Lentivirus packaging kits for SARS-CoV-2 coronavirus research

Lenti-Pac™ lentivirus packaging kits for SARS-CoV-2 coronavirus research

GeneCopoeia manufactures kits that allow researchers to produce SARS-CoV-2 Spike-pseudotyped lentivirus, which you can order from the table below.

To view the Spike mutations in each pseudovirus variant, refer to the table “Spike variant mutational profiles” below.

Buy Catalog number Product Description Spike variant1 Price
Kits for packaging lentivirus pseudotyped with full-length Spike protein
LT011 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) $806
LT015 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G $806
LT041 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (UK B.1.1.7 & D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent UK B.1.1.7 & D614G $806
LT044 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (South African B.1.351 & D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent South African B.1.351 & D614G $806

LT047

Coming soon!

 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (Brazil P.1 & D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent Brazil P.1 & D614G $806

LT061

Coming soon!

 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (India B.1.617) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent India B.1.617 $806

LT064

Coming soon!

 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (California B.1.427/B.1.429) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent California B.1.427/B.1.429 $806
Kits for packaging lentivirus pseudotyped with truncated Spike protein (ER retention signal removed)
LT021 Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) $806
LT017 Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G $806
Kits for packaging lentivirus with standard VSV-G glycoprotein
LT001 Lenti-Pac™ HIV Expression Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent N/A $641
LT002 Lenti-Pac™ HIV Expression Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent N/A $1070
Control lentiviral clones that can be packaged into lentiviral particles
EX-hLUC-Lv201 Control vector (with hLUC and eGFP) for pReceiver-Lv201 Control vector (with hLUC and eGFP) for pReceiver-Lv201 N/A $378
Additional reagents recommended for lentiviral packaging
LT005 Lenti-Pac™ HIV qRT-PCR Titration Kit (20 RT reactions, 50 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. N/A $308
LT006 Lenti-Pac™ HIV qRT-PCR Titration Kit (40 RT reactions, 100 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. N/A $465
LT007 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles N/A $151
LT008 Lenti-Pac™ 293Ta Cell Line (1.5 x 106 cells)-for packinging lentivirus with VSV-G only 293Ta Lentiviral packaging cell line-for packinging lentivirus with VSV-G only N/A $270
  SL221 HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells N/A inquiry
  SL222 HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2(NM_005656.3), 2×106 cells N/A inquiry
MP004 Mycoplasma PCR detection kit (50 rxns) Quick detection of mycoplasma contamination in cell culture or virus N/A $135

 

  User Manual
 

 LT011

 LT015

 LT041

  LT044  LT047 LT061 LT064

 LT017

  LT021

(LT001/LT002)

 

 

 

1SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

 

Spike variant mutational profiles

At the time of product introduction, GeneCopoeia’s R&D team used the GISAID database to determine the most frequently-occurring mutations in the Spike proteins from each variant to incorporate into our pseudovirus packaging kits. These mutational profiles are indicated below. Note that each variant can have different sub-lineages, which could contain a slightly different repertoire of mutations from the variants that we have made available. If you would like SARS-CoV-2 Spike-pseudotyped lentivirus packaging kits expressing a different set of mutations than we have currently available. please contact inquiry@genecopoeia.com for a custom project.

 

SARS-CoV-2 variant Spike mutations
Wild type (catalog number LT011, LT021) None
D614G (catalog number LT015, LT017) D614G
B.1.1.7 (first identified in the U.K.; catalog number LT041) Δ69-70, Δ144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
B.1.351 (first identified in South Africa; catalog number LT044) L18F, D80A, D215G，Δ242-244, K417N, E484K, N501Y, D614G, A701V
P.1 (first identified in Brazil; catalog number LT047) L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

HEK293T receptor cell lines expressing ACE2 and TMPRSS2

GeneCopoeia has produced HEK293T cell lines expressing human ACE2 alone, or ACE2 in combination with TMPRSS2, a protease that promotes Spike-ACE2 receptor interaction. These cell lines express ACE2 and/or TMPRRSS2 mRNA at high levels (Figure 1). Due to display of ACE2 protein (Figure 2) and/or TMPRSS2 protein ((Figure 3) on the cell surface, these cells are amenable to infection by viruses expressing SARS-CoV-2 Spike protein in their envelopes.

You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

In addition to the products below, custom stable cell lines are also available. For more information, please contact inquiry@genecopoeia.com.

Buy Catalog # Cell line Description Cell type Selection marker Price
SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $4321
SL222 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells Embryonic kidney

mCherry/

Puromycin,

Hygromycin

 $5186

 

  Warranty statement and limited use license   MSDS
 

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

Performance data

Figure 1. Relative mRNA expression levels of ACE2 and TMPRSS2 in GeneCopoeia stable cell lines. mRNA was amplified via RT-qPCR using primers specific for each gene. Ct values of ACE2 and TMPRSS2 were normalized to Ct values for housekeeping gene GAPDH. Relative expression levels were calculated from 2^ΔΔCt. A. ACE2 mRNA expression in parental cell line HEK293T and ACE2-overexpressing HEK293T cell line SL221. B. ACE2 and TMPRSS2 mRNA expression in parental cell line HEK293T and ACE2- and TMPRSS2-overexpressing cell line SL222.

 

Figure 2. Immunofluorescence (IF) analysis of ACE2 protein expression on the cell surface of ACE2-expressing HEK293T cells (Cat.# SL221), ACE2/TMPRSS2 expressing HEK293T cells (Cat.# SL222), and HEK293T cells. Unpermeablized cells were fixed, blocked and incubated with the primary anti-ACE2 antibody, then washed and incubated with  fluorescent-labeled secondary antibody, washed again, and stained with DAPI.

 

 

Figure 3. Immunofluorescence (IF) analysis of TMPRSS2 protein expression on the cell surface of TMPRSS2-expressing HEK293T cells (Cat.# SL222), and HEK293T cells. Unpermeablized cells were fixed, blocked and incubated with the primary anti-TMPRSS2 antibody, then washed and incubated with  fluorescent-labeled secondary antibody, washed again, and stained with DAPI.

 

Firefly luciferase assay kits

Luc-Pair™ Firefly Luciferase HS Assay Kit  

Buy Catalog # Product Description Price (US$)
LF007 Luc-Pair™ Firefly Luciferase HS Assay Kit (100rxns) High sensitivity for detecting the activity of Firefly luciferase. $86
LF008 Luc-Pair™ Firefly Luciferase HS Assay Kit (300rxns) $135
LF009 Luc-Pair™ Firefly Luciferase HS Assay Kit (1000rxns) $406
LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $44

  User manual MSDS

 

Gene Delivery

by | Jun 10, 2020 | Applications

The addition of exogenous nucleic acids to cells is a fundamental component of nearly every field of biological research. Scientists use nucleic acid delivery for many purposes, including editing or disrupting genes, overexpressing proteins for purification, and gene therapy. GeneCopoeia carries an extensive suite of reagents, from viral solutions to transfection reagents, that greatly aid your gene delivery applications.

 

Lentivirus

Infect most cell types

  

Adeno-associated Virus (AAV)

Safe, non-integrating ● High titers

     
     

Transfection Reagents

Efficient delivery to difficult-to-transfect cells

  
     
     

 

 

 

BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit

by | Jun 1, 2020 | Products

BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit with ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit with ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit with ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit with ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit without ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit without ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit without ROX dye
BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit without ROX dye

BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit with ROX dye BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit with ROX dye BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit without ROX dye BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit without ROX dye

Price: $860.00 $3,023.00 $860.00 $3,023.00
Catalog#:
  • QP201
  • QP202
  • QP203
  • QP204
Size: 200rxn 1,000rxn 200rxn 1,000rxn
Qty:
Manual: DownloadDownloadDownloadDownload
View your cart


GeneCopoeia’s BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit is a next-generation solution designed for highly sensitive and accurate detection of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. This RT-qPCR-based kit, which uses hydrolysis probe technology similar to TaqMan®, is newly designed from the ground up with primers that detect the S (Spike) and N (nucleocapsid) genes of multiple strains of SARS-CoV-2.

The BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit is part of GeneCopoeia’s COVID-19 Coronavirus Research Tools product line.

Advantages
  • High sensitivity.  Can detect as few as 10 copies of the viral target sequences.
  • Shorter reaction times. Uses GeneCopoeia’s BlazeTaq™ antibody-modified polymerase, which results in a faster hot start.
  • Broad-spectrum detection. Proprietary primer/probe designs enable detection of most SARS-CoV-2 strains.
  • Specificity. Inclusion of UDG and dUTP in the reaction reduces carryover contamination, resulting in fewer false positives.
  • Convenient. All required reagents are included in the kit, enabling one-step processing of samples to final data.
  • Instrument compatibility. Optional ROX dye ensures compatibility with virtually all qPCR instruments in use.

Note: The BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit is for research purposes only. It is not intended for diagnostic or treatment usage in humans.

For more information or to request a quote, please contact inquiry@genecopoeia.com.
Performance data Performance data

A

B
Figure 1. Performance of the BlazeTaq™ Probe One-Step SARS-CoV-2 Detection Kit. A. Cycle curves plotted against relative fluorescence units (RFU). B. Ct values for ten-fold serial dilutions of the positive control template. S: Spike gene. N: Nucleocapsid gene. GAPDH: Human reference gene. NTC: No template control. N/A: No amplification. Reactions were performed in duplicate.

COVID-19 Coronavirus Research Tools

by | Apr 6, 2020 | Products

General information

GeneCopoeia is your one-stop shopping solution for COVID-19 research tools.

For both SARS-CoV-2, the coronavirus responsible for the COVID-19 pandemic, as well as host factors like ACE2 and TMPRSS2, we provide:

SARS-CoV-2 Spike protein-pseudoypted lentivirus. For vaccine development, neutralizing antibody studies, and SARS-CoV-2 infection studies.
COVID-19 coronavirus receptor stable cell lines. For vaccine development, neutralizing antibody studies, and SARS-CoV-2 infection studies.
COVID-19 neutralizing antibody screening services. For vaccine development and neutralizing antibody studies. 
SARS-CoV-2 antigen microarrays. For detection of antibodies against SARS-CoV-2, influenza, and other viruses.
SARS-CoV-2 testing kits, RT-qPCR kits, and qPCR primers
Plasmid clones for ORF cDNA, gene promoters, and 3' UTR miRNA targets
Lentivirus and AAV, particles, which are high titer and ready-to-use
CRISPR and shRNA solutions

For more information or to request a quote, please contact inquiry@genecopoeia.com.

Looking for products related to SARS-CoV-1, the virus that caused the 2002-2004 SARS epidemic, or MERS-CoV, the virus that caused the MERS ourbreak beginning in 2012? Browse and select plasmid clones expressing the SARS-CoV-1 and MERS-CoV viral proteins here.