admin | GeneCopoeia™

One-step RT-qPCR Kits

by admin | Aug 2, 2019 | Products

A one-step RT-qPCR kit uses RNA as the starting material. It provides a convenient master mix to convert mRNA to cDNA and then quantify the cDNA in a one-step reaction. GeneCopoeia offers SYBR green- and probe-based one-step RT-qPCR kits that enable accurate analysis of gene expression in a convenient and high-throughput one-step format.

BlazeTaq™ SYBR Green One-Step RT-qPCR Kit

BlazeTaq™ One-Step SYBR Green RT-qPCR kit uses a reverse transcriptase to convert RNA to cDNA, and an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components.

BlazeTaq™ Probe One-Step RT-qPCR Kit

BlazeTaq™ Probe One-Step RT-qPCR kit allows potent target RNA detection and quantification using hydrolysis probes in a single tube. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components.

	SYBR green-based	Probe-based
Specificity	Medium	High
Reproducibility	Medium	High
Multiplexing	NO	Yes
Application	Gene expression DNA quantitation CHiP	Gene expression DNA quantitation CHiP SNP genotyping Copy number variation Pathway analysis Mutation detection

qPCR Mix & Primers

by admin | Aug 2, 2019 | Products, qPCR products

Quantitative PCR (qPCR) is one of the most widely-used methods for gene expression profiling, due to its sensitivity, speed, and relatively low cost. For the two-step real-time RT-qPCR system, GeneCopoeia provides three kinds of qPCR mix, experimentally-validated primers, and a first-strand cDNA synthesis kit for you to detect and quantitate your gene expression.

BlazeTaq™ SYBR Green qPCR Mix 2.0

It contains a hot-start antibody-modified Taq DNA polymerase and an optimized buffer system that avoids non-specific amplification of target DNA at lower temperatures and enhances reaction mix performance.

BlazeTaq™ Probe qPCR Mix

It uses hydrolysis probes and DNA as starting material. The kit is supplied with a ready-to-use 5X master mix with a hot-start Taq DNA polymerase, dNTP and all optimized buffer components.

All-in-One™ qPCR Mix and Validated Primers

It uses high-fidelity hot-start polymerase, an optimized reaction buffer and high-quality dNTPs to enable specific and sensitive amplification from even low-copy RNA (cDNA) or DNA species.

SureScript™ First-Strand cDNA Synthesis Kits

The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.

	SYBR green-based	Probe-based
Specificity	Medium	High
Reproducibility	Medium	High
Multiplexing	No	Yes
Application	Gene expression DNA quantitation CHiP	Gene expression DNA quantitation CHiP SNP genotyping Copy number variation Pathway analysis Mutation detection

*Promotion is valid in the US & Canada only. For international customers, please contact your local distributors. Promotion ends July 31, 2023. Discounts are not valid on previous purchases and cannot be combined with additional discounts.

Custom Services

by admin | Jul 17, 2019 | Custom services, Products

GeneCopoeia offers high quality and comprehensive custom services, including custom cloning services for ORF cDNAs, promoter reporters, miRNA 3’ UTR targets, CRISPR and shRNAs. Gene synthesis services, high titer and ultra purified lentivirus production services as well as mammalian stable cell line development services for recombinant protein and antibody production, drug screening, and other applications are also available.

	Antigen array screening services GeneCopoeia’s OmicsArray™ antigen microarrays are powerful tools for many applications, including autoantibody detection, biomarker profiling, and characterization of protein-molecule interactions.
	Custom cloning services GeneCopoeia is your premier and economical source for custom clone construction for ORF cDNA, gene reporters, miRNA 3’ UTR targets, shRNA and CRISPR.
	Custom gene synthesis services Fast turnaround custom gene synthesis services Competitive pricing
	Custom circRNA synthesis services GeneCopoeia provides custom in vitro circRNA synthesis service and premade circRNA products.
	Mammalian stable cell line development services GeneCopoeia offers state-of-the-art services for establishing stable cell lines for protein overexpression, gene knockdown or genome editing that meet your specific research needs.
	Cell line characterization services GeneCopoeia offers cell line characterization reagent kits and services that involve testing for a combination of important genetic markers, including chromosomes, DNA fingerprints, alternative splicing and genes of your interest to analyze genetic variation, exploring transcriptome profiles, and assure culture sterility.

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Custom Cloning Services

by admin | Jul 3, 2019 | Custom services, Products

GeneCopoeia is your premier and economical source for custom clone construction for ORF cDNAs, gene reporters, miRNA 3’ UTR targets, shRNA and CRISPR. We provide ease and confidence for your gene functional studies with low research cost.

Advantages

Best variety of vector choices – Hundreds of vector options available.
Reliable results – Customized constructs are fully sequence-verified and guaranteed.
Lentivirus and AAV options – Viral packaging services for your customized constructs are also available in convenient ready-to-transduce formats!

Available services

Nucleotide base substitutions
Insertion/deletion mutations
Vector backbone modifications
Codon optimization
Large plasmid preps
Other customized sequences

Secrete-Pair™ Gaussia and SEAP Dual Luminescence Assay Kit

by admin | Jul 19, 2017 | landing

Secrete-Pair™ Dual Luminescence Assay Kit

– Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) dual-reporter system

Secreted reporters enable live cell analysis. Ideal for time point studies and high-throughput screening.
SEAP allows normalization for greater accuracy.
Compatible with GeneCopoeia’s promoter clones, miRNA target clones, & cloning vectors.
Gaussia luciferase reporter vectors – Only $49 each when purchased together with Secrete-Pair™ Luciferase Assay Kits*.