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Complete List of Cas9 Cell Lines

by | Jul 17, 2017 | landing

Pre-made CRISPR-Cas9 Stable Cell Lines

– Largest Collection On The Market

  • Stable Cas9 integration eliminates need for the sgRNAs co-transfection. Ideal for high-throughput applications.
  • Single clone isolation provides robust Cas9 expression in a uniform background.
  • Cas9 functionally validated using the IndelCheck™ T7 Endonuclease I assay.
  • Available for human, mouse and rat


  • Applications include:
  • CRISPR sgRNA or TALEN functional validation
  • Screening cell clones for knockout (KO) and knock-in (KI) modifications
  • Fast turnaround
  • For human, mouse and rat
  • $60/ Cas-9 clone; $149/ sgRNA clone

Contact us:
Phone: 1-301-762-0888
Email: inquiry@genecopoeia.com
www.genecopoeia.com

Contact your local distributor for pricing information.

 

 

Lentifect™ Ultra-purified Lentivirus

by | Jul 11, 2017 | Products

For in vivo (animal) applications

Overview GeneCopoeia’s Lentifect™ Ultra-purified Lentivirus is serum free and is engineered for in vivo use, which is subjected to a multi-step purification process, resulting in high titers and ultra-high purity. This procedure ensures that viral preparations are free of contaminants that can cause undesired immunological responses and cytotoxicity, and is suitable for use in animal models. Lentifect™ custom ultra-purified lentivirus production services are available for nearly any GeneCopoeia clone type, including:
  • ORF
  • CRISPR sgRNA
  • shRNA
  • miRNA precursors
  • miRNA inhibitors
  • Promoters
 Search lentivirus
To order custom Lentifect™ ultra-purified lentiviral particles visit our search page.

Advantages

  • Ultra-purified and serum free. Very low levels of plasmid, cell debris or protein residue.
  • High titer. ≥ 10^8 TU/ml. (Sufficient for most in vivo experiments)
  • Cloning included. Large collection of clones, including ORF, shRNA, sgRNA, miRNA and promoters, with a wide choice of promoters, selection markers, and reporters.
  Looking for in vitro grade lentivirus for your cell line work? GeneCopoeia also provides Lentifect™ purified lentiviral particles for in vitro use.

ORF Mutant Clones

by | Jun 28, 2017 | landing

GeneCopoeia’s mutant ORF clones enable you to generate any custom modifications to a wild type ORF cDNA sequence. In combination with our vast collection of OmicsLink™ Expression-Ready ORF Clones, it provides ease and confidence for your gene function studies, with low research cost.

Advantages include:

  • Flexible services – Site mutations, insertions, deletions
  • Variety of vector choices – 200+ vectors available
  • Reliable result – Fully sequence-verified and guaranteed
  • Lentivirus option – Lentiviral packaging service is available

Customer receives:

  • Mutant ORF clone in plasmid (10ug) or bacterial stock format
  • Clone datasheet
  • Sequencing data upon request

Available vectors:

Vector Promoter Host Cell Selection Marker Tag Protease Site
pShuttle™ N/A Gateway PLUS shuttle N/A Untagged N/A
pShuttle ™ N/A Gateway PLUS shuttle
(no stop codon)		 N/A Untagged N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-B01 T7 Bacterial N/A N-His N/A
pReceiver-B31 T7 Bacterial N/A C-His N/A
pReceiver-B02 T7 Bacterial N/A Untagged N/A
pReceiver-B03 T7 Bacterial N/A N-GST Tev
pReceiver-B04 T7 Bacterial N/A N-GST EK
pReceiver-B05 Tac Bacterial N/A N-GST Tev
pReceiver-B06 Tac Bacterial N/A N-GST EK
pReceiver-B07 Tac Bacterial N/A N-MBP Tev
pReceiver-B08 Tac Bacterial N/A N-MBP EK
pReceiver-B09 T7 Bacterial N/A N-Avi N/A
pReceiver-B10 Tac Bacterial N/A N-Flag N/A
pReceiver-B11 Tac Bacterial N/A N-His N/A
pReceiver-B12 Tac Bacterial N/A His-SUMO Sumo protease
pReceiver-B13 T7 Bacterial N/A His-SUMO SUMO Protease
pReceiver-B80 T7 Bacterial N/A N-HaloTag Tev
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-M34 CAG Mammalian Neomycin C-Flag N/A
pReceiver-M35 CMV Mammalian Neomycin C-Flag N/A
pReceiver-M37 CAG Mammalian Neomycin C-eGFP N/A
pReceiver-M39 CMV Mammalian Puromycin C-Flag N/A
pReceiver-M62 CMV Mammalian Neomycin C-Avi-IRES-Biotin ligase N/A
pReceiver-M73 CMV Mammalian Neomycin C-Myc-IRES2-mCherry N/A
pReceiver-M83 CMV Mammalian Neomycin IRES2-mCherry N/A
pReceiver-M90 CMV Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-M91 CMV Mammalian Puromycin C-3xHA-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M92 CMV Mammalian Puromycin C-Myc-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M93 CMV Mammalian Puromycin C-Flag-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M94 EF1a Mammalian Puromycin Untagged N/A
pReceiver-M95 CMV Mammalian Puromycin SV40-mCherry-IRES-Puromycin N/A
pReceiver-M97 EF1a Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M99 CMV Mammalian Neomycin C-mEOS3 N/A
pReceiver-M01 CMV Mammalian Neomycin N-His N/A
pReceiver-M51 CMV Mammalian Neomycin C-His+IRES-eGFP N/A
pReceiver-M77 CMV Mammalian Neomycin C-His N/A
pReceiver-M02 CMV Mammalian Neomycin Untagged N/A
pReceiver-M67 CMV Mammalian Hygromycin Untagged N/A
pReceiver-M68 CMV Mammalian Puromycin Untagged N/A
pReceiver-M29 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-M03 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-M98 CMV Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M15 CMV Mammalian Neomycin N-eYFP N/A
pReceiver-M16 CMV Mammalian Neomycin C-eYFP N/A
pReceiver-M32 CMV Mammalian Neomycin N-eCFP N/A
pReceiver-M33 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-M04 CMV Mammalian Neomycin N-GST EK
pReceiver-M05 CMV Mammalian Neomycin N-Avi N/A
pReceiver-M48 CMV Mammalian Neomycin N-Avi+IRES-Biotin ligase N/A
pReceiver-M17 CMV Mammalian Neomycin C-Avi N/A
pReceiver-M06 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-M07 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-M08 CMV Mammalian Neomycin C-3xHA-His N/A
pReceiver-M43 CMV Mammalian Neomycin N-Myc N/A
pReceiver-M45 CMV Mammalian Neomycin C-3xHA+IRES-eGFP N/A
pReceiver-M09 CMV Mammalian Neomycin C-Myc N/A
pReceiver-M10 CMV Mammalian Neomycin C-Myc-His N/A
pReceiver-M72 CMV Mammalian Neomycin C-Myc+IRES2-eGFP N/A
pReceiver-M11 CMV Mammalian Neomycin N-Flag N/A
pReceiver-M12 CMV Mammalian Neomycin N-3XFlag N/A
pReceiver-M46 CMV Mammalian Neomycin C-Flag+IRES-eGFP N/A
pReceiver-M14 CMV Mammalian Neomycin C-3XFlag N/A
pReceiver-M49 CMV Mammalian Neomycin N-HaloTag Tev protease
pReceiver-M50 CMV Mammalian Neomycin C-HaloTag Tev protease
pReceiver-M55 CMV Mammalian Neomycin N-mCherry N/A
pReceiver-M56 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-M61 CMV Mammalian Neomycin IRES2-eGFP N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Lv117 CMV Mammalian Puromycin N-3xHA N/A
pReceiver-Lv118 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-Lv145 EF1a Mammalian Hygromycin SV40-hygromycin N/A
pReceiver-Lv163 EF1a Mammalian Neomycin SV40-neomycin N/A
pReceiver-Lv170 CMV Mammalian Neomycin C-Myc N/A
pReceiver-Lv181 CMV Mammalian Puromycin N-3xFlag N/A
pReceiver-Lv185 PGK Mammalian Puromycin Untagged N/A
pReceiver-Lv188 CMV Mammalian N/A C-eGFP N/A
pReceiver-Lv193 CMV Mammalian Puromycin C-eGFP(monomeric) N/A
pReceiver-Lv195 EF1a Mammalian Puromycin SV40-puromycin N/A
pReceiver-Lv196 TRE3G Mammalian Puromycin SV40-puromycin Tet-On 3G System
pReceiver-Lv197 CMV Mammalian Blasticidin Untagged N/A
pReceiver-Lv208 TRE3G Mammalian Puromycin SV40-rtTA-T2A-eGFP-IRES-Puro Tet-On 3G System
pReceiver-Lv209 TRE3G Mammalian Puromycin SV40-eGFP-IRES-Puro Tet-On 3G System
pReceiver-Lv212 TRE3G Mammalian Puromycin SV40-rtTA-IRES-Puro Tet-On 3G System
pReceiver-Lv218 CMV Mammalian Neomycin IRES2-Neomycin N/A
pReceiver-Lv219 CMV Mammalian Puromycin IRES2-Puromycin N/A
pReceiver-Lv221 CMV Mammalian N/A IRES2-eYFP N/A
pReceiver-Lv222 CMV Mammalian N/A IRES2-eCFP N/A
pReceiver-Lv233 EF1a Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv234 CAG Mammalian N/A IRES2-eGFP N/A
pReceiver-Lv235 CAG Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv236 CAG Mammalian Puromycin Untagged N/A
pReceiver-Lv237 CAG Mammalian Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv241 CMV Mammalian Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv243 CAG Mammalian Puromycin C-Avi-Flag-SV40-Puromycin N/A
pReceiver-Lv244 CMV Mammalian Hygromycin SV40-mCherry-IRES-Hygromycin N/A
pReceiver-Lv245 EF1a Mammalian Puromycin cMyc-SV40-mCherry-IRES-Puromycin N/A
pReceiver-Lv246 UBC Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv247 UBC Mammalian Puromycin IRES2-mCherry-IRES-Puromycin N/A
pReceiver-Lv248 TRE3G Mammalian Puromycin SV40-tTA-T2A-eGFP-IRES-Puro Tet-Off System
pReceiver-Lv251 CMV Mammalian Puromycin C-3XFlag-SV40-Puromycin N/A
pReceiver-Lv253 EF1a Mammalian Puromycin SV40-mcherry-IRES-Puromycin N/A
pReceiver-Lv254 EF1a Mammalian Neomycin SV40-mcherry-IRES-Neomycin N/A
pReceiver-Lv105 CMV Mammalian Puromycin Untagged N/A
pReceiver-Lv151 CMV Mammalian Neomycin Untagged N/A
pReceiver-Lv152 CMV Mammalian Hygromycin Untagged N/A
pReceiver-Lv153 CMV Mammalian N/A Untagged N/A
pReceiver-Lv186 CMV Mammalian N/A C-3xHA N/A
pReceiver-Lv157 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-Lv120 CMV Mammalian Puromycin C-3xHA N/A
pReceiver-Lv108 CMV Mammalian Puromycin N-Avi N/A
pReceiver-Lv124 CMV Mammalian Puromycin C-Avi N/A
pReceiver-Lv242 CMV Mammalian Puromycin C-Avi-FLAG N/A
pReceiver-Lv154 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-Lv127 CMV Mammalian Puromycin C-eCFP N/A
pReceiver-Lv109 CMV Mammalian Puromycin N-eCFP N/A
pReceiver-Lv103 CMV Mammalian Puromycin N-eGFP N/A
pReceiver-Lv122 CMV Mammalian Puromycin C-eGFP N/A
pReceiver-Lv182 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-Lv183 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-Lv189 CMV Mammalian N/A N-eGFP N/A
pReceiver-Lv123 CMV Mammalian Puromycin C-eYFP N/A
pReceiver-Lv104 CMV Mammalian Puromycin N-eYFP N/A
pReceiver-Lv187 CMV Mammalian N/A C-FLAG N/A
pReceiver-Lv101 CMV Mammalian Neomycin N-Flag N/A
pReceiver-Lv102 CMV Mammalian Puromycin N-Flag N/A
pReceiver-Lv158 CMV Mammalian Neomycin C-FLAG N/A
pReceiver-Lv203 CMV Mammalian Puromycin C-Flag-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv216 CMV Mammalian Puromycin C-Flag-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv129 CMV Mammalian Puromycin C-HaloTag Tev
pReceiver-Lv110 CMV Mammalian Puromycin N-HaloTag Tev
pReceiver-Lv211 CMV Mammalian Hygromycin C-HaloTag-IRES-hygromycin N/A
pReceiver-Lv128 CMV Mammalian Puromycin C-His N/A
pReceiver-Lv215 CMV Mammalian N/A IRES2-eGFP N/A
pReceiver-Lv205 CMV Mammalian Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv220 CMV Mammalian Hygromycin IRES2-Hygromycin N/A
pReceiver-Lv214 CMV Mammalian N/A IRES2-mCherry N/A
pReceiver-Lv213 CMV Mammalian Puromycin IRES2-mCherry-IRES-puromycin N/A
pReceiver-Lv111 CMV Mammalian Puromycin N-mCherry N/A
pReceiver-Lv130 CMV Mammalian Puromycin C-mCherry N/A
pReceiver-Lv155 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-Lv107 CMV Mammalian Puromycin N-Myc N/A
pReceiver-Lv125 CMV Mammalian Puromycin C-Myc N/A
pReceiver-Lv126 CMV Mammalian Puromycin C-Myc-His N/A
pReceiver-Lv202 CMV Mammalian Puromycin C-Myc-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv204 CMV Mammalian Puromycin C-Myc-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv207 CMV Mammalian Hygromycin SV40-eGFP-IRES-hygromycin N/A
pReceiver-Lv201 CMV Mammalian Puromycin SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv217 CMV Mammalian Puromycin SV40-hLUC-IRES-puromycin N/A
pReceiver-Lv206 CMV Mammalian Puromycin SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv171 EF1a Mammalian N/A IRES-eGFP N/A
pReceiver-Lv173 EF1a Mammalian N/A IRES-mcherry N/A
pReceiver-Lv180 EF1a Mammalian Puromycin IRES-mcherry-SV40-puromycin N/A
pReceiver-Lv179 EF1a Mammalian Puromycin IRES-eGFP-SV40-puromycin N/A
pReceiver-Lv228 PGK Mammalian N/A IRES2-eGFP N/A
pReceiver-Lv229 PGK Mammalian N/A IRES2-mcherry N/A
pReceiver-Lv230 PGK Mammalian Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv231 PGK Mammalian Puromycin IRES2-eGFP-IRES-puromycin N/A
pLX304 CMV Mammalian Blasticidin C-V5 N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Y01 GAL1 Yeast N/A C-His N/A
pReceiver-YAD pADH Yeast N/A GAL4AD N/A
pReceiver-YBD pADH Yeast N/A GAL4DB N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-I01 AcMNPV polyhedrin Insect cell N/A N-His Tev
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-WG02 T7 cell free N/A N-His Factor Xa
pReceiver-WG03 T7 cell free N/A N-His-SUMO CoolCutter(TM)
pReceiver-WG04 T7 cell free N/A N-Avi-SUMO CoolCutter(TM)
pReceiver-WG05 T7 cell free N/A N-His-Avi-SUMO CoolCutter(TM)
pReceiver-WG09 T7 cell free N/A His-GST TEV
pReceiver-WG16 T7 cell free N/A N/A N/A
pReceiver-WG31 T7 cell free N/A N-His-SUMO-Avi CoolCutter(TM)
pReceiver-WG33 T7 cell free N/A N-Trx-His-SUMO CoolCutter(TM)

How to order:

Send your gene information and service requirement to inquiry@genecopoeia.com to request a quote.

Search ORF Clone

Luc-Pair™ Firefly Luciferase HT Assay Kit

by | Feb 24, 2017 | Products

Luc-Pair™ Firefly Luciferase HT Assay Kit
Luc-Pair™ Firefly Luciferase HT Assay Kit
Luc-Pair™ Firefly Luciferase HT Assay Kit
Luc-Pair™ Firefly Luciferase HT Assay Kit
Luc-Pair™ Firefly Luciferase HT Assay Kit
Luc-Pair™ Firefly Luciferase HT Assay Kit
pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector

Luc-Pair™ Firefly Luciferase HT Assay Kit Luc-Pair™ Firefly Luciferase HT Assay Kit Luc-Pair™ Firefly Luciferase HT Assay Kit pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector

Price: $86.00 $140.00 $422.00 $536.00 $536.00 $536.00
Catalog#:
  • LF016
  • LF017
  • LF018
  • ZX001
  • ZX002
  • ZX003
Size: 10ml 30ml 100ml (10µg) (10µg) (10µg)
Qty:
Manual: DownloadDownloadDownloadDownloadDownloadDownload
View your cart


– For High-Throughput Applications

Introduction

GeneCopoeia’s Luc-Pair™ Firefly Luciferase HT Assay Kit is a convenient system for high-throughput detection of Firefly luciferase (FLuc) activity in 96- or 384-well plates. The Luc-Pair™ Firefly Luciferase HT Assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing serum (e.g. DMEM, RPMI1640, etc.).

The Luc-Pair™ Firefly Luciferase HT Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.)  and is compatible with many Fluc vectors from GeneCopoeia, Promega and other sources.

Advantages

· Enhanced stability – Greater signal stability, with a half-life of approximately 2 hours (Figure 1). Ideal  for high throughput assays. · Convenience — Directly lyse cells in culture medium and measure luciferase activity simultaneously. · Flexibility — Works well in many types of culture media, even with serum present (Figure 2). · Versatility — Works well with many different eukaryotic (adherent or suspension) cells using micro-plate luminescence readers. · Low background — Very limited background luminescence. No subtraction from readings is required. · Reproducibility — Reliable, linear results for a concentration range over several orders of magnitude.
The Luc-Pair™ Firefly Luciferase HT Assay Kit provices excellent FLuc signal stability, with a half life of approximately 2 hours (Figure 1).

Figure 1.  Stability of Firefly luciferase signal. HEK 293 cells were transfected with Promega pGL4.13 reporter vector for 48 hours. Fluc activity was measured at various time points using the Luc-Pair FLuc HT Assay Kit as described in the procedure. 

The Luc-Pair™ Firefly Luciferase HT Assay Kit allows excellent signal strength and stability in many different types of culture media, even with serum present (Figure 2).

Figure 2. Firefly luciferase activities in various media. HEK 293 cells were transfected with Promega pGL4.13/pGL4.75 reporter vectors for 48 hours. 5×104 transfectd cells were suspended in DMEM without serum or the following types of media containing 10% serum:  DMEM, RPMI1640, EMEM, IMDM, McCoy’s5A, F-12K, MEBM, ACL-4, L15, Cho-S-SFMII, NCTC109. Firefly luciferase activities were measuredat various time points using the Luc-Pair FLuc HT Assay Kit as described in the procedure.

MSDS

     

Luc-Pair™ Renilla Luciferase HT Assay Kit

by | Feb 24, 2017 | Products

Luc-Pair™ Renilla Luciferase HT Assay Kit
Luc-Pair™ Renilla Luciferase HT Assay Kit
Luc-Pair™ Renilla Luciferase HT Assay Kit
Luc-Pair™ Renilla Luciferase HT Assay Kit
Luc-Pair™ Renilla Luciferase HT Assay Kit
Luc-Pair™ Renilla Luciferase HT Assay Kit
pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector
pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector

Luc-Pair™ Renilla Luciferase HT Assay Kit Luc-Pair™ Renilla Luciferase HT Assay Kit Luc-Pair™ Renilla Luciferase HT Assay Kit pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) cloning vector reporter cloning vector

Price: $86.00 $140.00 $422.00 $536.00 $536.00 $536.00
Catalog#:
  • LF019
  • LF020
  • LF021
  • ZX001
  • ZX002
  • ZX003
Size: 10ml 30ml 100ml (10µg) (10µg) (10µg)
Qty:
Manual: DownloadDownloadDownloadDownloadDownloadDownload
View your cart


– For High-Throughput Applications

Introduction

GeneCopoeia’s Luc-Pair™ Renilla Luciferase HT Assay Kit is a convenient system for high-throughput detection of Renilla luciferase (RLuc) activity in 96- or 384-well plates. The Luc-Pair™ Renilla Luciferase HT Assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing serum (e.g. DMEM, RPMI1640, etc.) 

The Luc-Pair™ Renilla Luciferase HT Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.)  and is compatible with many Rluc vectors from GeneCopoeia, Promega and other sources.  

Advantages

  • Enhanced stability – Greater signal stability, with a half-life of approximately one hour (Figure 1). Ideal  for high throughput assays.
  • Convenience — Directly lyse cells in culture medium and measure luciferase activity simultaneously.
  • Flexibility — Works well in many types of culture media, even with serum present (Figure 2).
  • Versatility — Works well with many different eukaryotic (adherent or suspension) cells using microplate luminescence readers.
  • Low background — Very limited background luminescence. No subtraction from readings is required.
  • Reproducibility — Reliable, linear results for a concentration range over several orders of magnitude.
The Luc-Pair™ Renilla Luciferase HT Assay Kit provices excellent RLuc signal stability, with a half life of approximately 1 hour (Figure 1).
Figure 1.  Stability of Renilla luciferase signal. HEK 293 cells were transfected with Promega pGL4.75 reporter vector for 48 hours. Rluc activity was measured at various time points using the Luc-Pair RLuc HT Assay Kit as described in the procedure. 
The Luc-Pair™ Renilla Luciferase HT Assay Kit allows excellent signal strength and stability in many different types of culture media, even with serum present (Figure 2).
Figure 2. Renilla luciferase activities in various media. HEK 293 cells were transfected with Promega pGL4.75 reporter vector for 48 hours. 5×10^4 transfectd cells were suspended in DMEM without serum or the following types of media containing 10% serum:  DMEM, RPMI1640, EMEM, IMDM, McCoy’s5A, F-12K, MEBM, ACL-4, L15, Cho-S-SFMII, NCTC109. Renilla luciferase activities were measured at various time points using the Luc-Pair RLuc HT Assay Kit as described in the procedure.

MSDS