by admin | Sep 26, 2012 | FAQs
Answer: Yes, and there are two choices. First, you can use the OmicsLink™ Expression Clones that we have developed. We have already constructed ORF clones in expression vectors with N-terminal tags or C-terminal tags, such as His tag and eGFP, there are more than 10 types of targs to meet your need. Please check OmicsLink™ product information page (https://www.genecopoeia.com/tech/omicslik/). Second, you may also use Invitrogen's Gateway® expression vectors for expressing N-terminal or C-terminal fusion proteins. Because our ORFEXPRESS™ Gateway® Shuttle Clones contains stop codons, you will need to remove the stop codons before transferring ORF inserts into Invitrogen's pDEST expression vectors to produce C-terminal tagged fusion proteins.
by admin | Sep 26, 2012 | FAQs
Answer: The selection procedure is very stringent. It includes extraction, comparison and validation of gene sequences and their annotation information from multiple public and private sources. Clustering and manual curation are applied to reduce redundancy and eliminate erroneous genes.
by admin | Sep 26, 2012 | FAQs
Answer: There is a suite of pDEST expression vectors (destination vectors) available from Invitrogen Corporation that can produce various forms of proteins (such as native, N-terminal fusion or C-terminal fusion) in different host cell systems including bacterial, yeast, insect and mammalian cell systems.
by admin | Sep 26, 2012 | FAQs
Answer: The vector for ORFEXPRESS™ Gateway® Shuttle Clones manufactured by GeneCopoeia, Inc. (GCI) harbors full length ORFs flanked by attL1 and attL2 recombination sites that pair with the Gateway® pDEST expression vectors containing attR1 and attR2 recombination sites. When GCI's ORFEXPRESS™ Shuttle Clones are mixed with Gateway® compatible pDEST expression vector(s), with optimal concentrations of enzymes and conditions, the ORFs in the ORFEXPRESS™ Shuttle clones can be transferred into Gateway® pDEST expression vector(s). Because of the unique feature of recombination sites in the vectors, the transferred ORFs will be under the control of the promoter(s) in the destination expression vector(s).
by admin | Sep 26, 2012 | FAQs
Answer: The full-length ORF clones contain only the protein coding sequences, while other full-length whole transcript cDNA clones contain non-coding sequences, such as 5' and/or 3' UTRs, which have been known to have possible negative impact on the protein translation process. Please visit this page for further details on the comparison of these two types of clones: (https://www.genecopoeia.com/tech/application/).
