by admin | Sep 26, 2012 | FAQs
Answer: Yes, we will clone the gene(s) for you. It is our mission and continuing effort to clone all full-length ORFs of human genes. It is likely that the gene(s) that you are interested in is already in our production pipeline or in the queue for future cloning. If you provide us with the GenBank accession, gene name, nucleotide or amino acid sequences, we will place the gene(s) in the list of priority genes to be cloned. Usually, it takes two weeks to complete the cloning, although we cannot guarantee that we will get the gene(s). You will be notified as soon as the clone(s) becomes available. There will be no additional charge.
by admin | Sep 26, 2012 | FAQs
Answer: There are usually multiple gene transcripts in a gene locus, resulting from alternative splicing, updated information about ORFs, polymorphism, or redundancies from duplication of cloning effort from different laboratory groups. During the process of selecting full-length genes for ORF cloning, we try to choose the most representative transcript from each gene locus, which may differ slightly or significantly from other transcripts in the same gene locus. This approach provides the broadest coverage of all gene loci. In many instances, we do obtain multiple variants or versions of full length ORFs from our cloning production pipeline. Customers are encouraged to send inquires to inquiry@genecopoeia.com if he/she is interested in obtaining a particular variant/version of a gene/ORF.
In some cases, discrepancies could be originated from changed annotation of ORF start and/or stop location as the result of newly available sequence information and/or experimental evidence. On regular basis, we check for information updates on ORF annotations in public databases such as NCBI and EMBL. The new ORF annotations will then be used in our recloning effort to keep our ORF clones most updated.
by admin | Sep 26, 2012 | FAQs
Answer: No, the sequence on the website is not the actual sequence but the reference sequence from NCBI. The actual sequence of the clone may differ due to genetic variation and alternative splicing. The actual sequence for any of the ORF cDNA clones can be requested at any time which can be sent by e-mail. However, please note that the actual sequence for clones in pre-production or ones offered via Custom Gene Synthesis may not yet be available.
by admin | Sep 26, 2012 | FAQs
The sequences are different depending on which full length ORF clone for both ORFEXPRESS™ Gateway® Shuttle Clones and OmicsLink™ Expression Clones you purchased. First check the datasheet which is shipped with the clone and use the figure to identify the sequence.
fig. 1. Example: Multiple cloning site of Catalog No. GC-B0128
by admin | Sep 26, 2012 | FAQs
Answer: Ampicillin or Neomycin. Please check carefully when you order OmicsLink™ Expression Clones (https://www.genecopoeia.com/tech/omicslink/).
