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GeneHero™ CRISPR Products and Services

GeneCopoeia's GeneHero™ CRISPR-Cas9 products and services provide a complete, powerful solution to your genome editing needs. Products and services include:

  • CRISPR Plasmids

Transfect cells with our CRISPR plasmids with Cas9 and sgRNA for human, mouse, and rat. Search our database of more than 45,000 human, mouse, and rat genes for genome editing using CRISPR. 

  • CRISPR Lentivirus

Genome integration of CRISPR elements using lentivirus. Cas9 and/or sgRNA packed in purified lentiviral particles at 108TU/ml, ready to infect all cell types.

  • CRISPR AAV

Episomal expression of CRISPR components with adeno-associated viral particles carrying Cas9 and/or sgRNA, excellent for tissue and animal transduction.

Premade Cas9-expressing stable cell lines are great for sgRNA library screening and other high-throughput CRISPR-Cas9 applications.

Advantages

  • RNA-guided genomic DNA recognition regardless of the methylation status
  • Similar or greater gene-editing efficiency compared to ZFNs and TALENs
  • Capable of editing multiple genes simultaneously (multiplexing)
  • Simple and fast design process. No need to reengineer the nuclease for each new target

Product Information

CRISPR plasmids

sgRNA expression plasmids are available for targeting virtually any gene in any experimental system. sgRNA plasmids express either sgRNA only, or sgRNA + Cas9 nuclease in an all-in-one format. Lentiviral clones express sgRNA alone. Cas9-expressing lentiviral clones are available separately.

The CRISPR plasmid options listed below are intended for using 20 ntsgRNAs with the wild-type Cas9 nuclease from Streptococcus pyogenes(SpCas9). GeneCopoeia also provides alternate CRISPR options, such as Cas9 D10A nickase and other high-fidelity versions of Cas9, SaCas9, and shorter sgRNAs. To purchase reagents for these other options, please contact usfor a custom quote.

Custom sgRNA Expression Plasmids

Vector Promoter sgRNA and Cas9 Selection Marker/ Reporter Gene Vector Type Vector Map
All-in-one (Cas9 nuclease + sgRNA) clones
pCRISPR-CG02 U6 sgRNA and CBh-driven Cas9 (wild type) in the same vector N/A Non-viral get_info
pCRISPR-CG04 U6 sgRNA and CMV-driven Cas9 (wild type) in the same vector Neomycin / copGFP Non-viral get_info
pCRISPR-CG07 U6 sgRNA and CBh-driven Cas9 (wild type) in the same vector copGFP Non-viral get_info
pCRISPR-CG08 U6 sgRNA and CBh-driven Cas9 (wild type) in the same vector mCherry Non-viral get_info
pCRISPR-CG12 U6 sgRNA and CMV-driven Cas9 (wild type) in the same vector Neomycin / mCherry Non-viral get_info
sgRNA only clones
pCRISPR-SG01 U6 sgRNA only Hygromycin Non-viral get_info
pCRISPR-LvSG03 U6 sgRNA only Puromycin / mCherry Lentiviral get_info

Cas9 Nuclease Expression Plasmids

Clones expressing human codon-optimized S. pyogenes Cas9 nuclease or D10A nickase are available. These clones do not express sgRNAs.

Buy Catalog# Product Promoter Reporter gene / Selection marker Price
Cas9 nuclease and D10A nickase expression clones
CP-C9NU-01 Cas9 nuclease expression clone CMV mCherry / Neomycin $595*
CP-C9NI-01 Cas9 D10A nickase expression clone CBh N/A $595*
CP-C9NI-02 Cas9 D10A nickase expression clone CMV mCherry / Neomycin $595*
Cas9 nuclease lentiviral expression clones
CP-LvC9NU-01 Cas9 nuclease lentiviral expression clone CMV Neomycin $595*
CP-LvC9NU-02 Cas9 nuclease lentiviral expression clone CMV eGFP / Neomycin $595*
CP-LvC9NU-08 Cas9 nuclease lentiviral expression clone EF1a Puromycin $595*
CP-LvC9NU-09 Cas9 nuclease lentiviral expression clone EF1a eGFP/Neomycin $595*
CP-LvC9NU-10 Cas9 nuclease lentiviral expression clone EF1a eGFP/Hygromycin $595*

 * Discount applied when purchased together with a sgRNA clone.  

Click here to search our database of sgRNA plasmids for knocking out more than 45,000 human, mouse, and rat genes.