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Validated All-in-One™ qPCR Primer for CLEC4M(NM_014257.4) Search again
Product ID:
HQP000411
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD209L, CD299, DC-SIGN2, DC-SIGNR, DCSIGNR, HP10347, L-SIGN, LSIGN
Gene Description:
C-type lectin domain family 4 member M
Target Gene Accession:
NM_014257.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity.
Gene References into function
- Crystal structures of carbohydrate-recognition domains of DC-SIGNR bound to oligosaccharide, in combination with binding studies, reveal that it selectively recognizes endogenous high-mannose oligosaccharides
- mediates cellular entry by Ebola virus in cis and in trans
- reasons underlying the restricted distribution of DC-SIGNR; and expression in relation to HIV entry receptors
- Review. This article reviews the interaction of DC-SIGN & DC-SIGNR with HIV and Ebola & discusses the mechanism of DC-SIGN-mediated viral transmission.
- the influx or proliferation of DC-SIGN+ immature and mature DCs and L-SIGN+ cells is dynamically regulated
- data demonstrate for the first time that lectins DC-SIGN and L-SIGN differ in their carbohydrate binding profiles
- largely expressed on endothelial cells in liver sinusoids; results demonstrate that hepatitis C pseudoviruses captured by L-SIGN+ or DC-SIGN+ cells efficiently transinfect adjacent human liver cells
- can serve as an alternative receptor for SARS coronavirus
- crystal forms of truncated DC-SIGNR comprising two neck repeats and the carbohydrate-recognition domain reveal that the CRDs are flexibly linked to the neck, which contains alpha-helical segments interspersed with non-helical regions
- crystal structure of DC-SIGNR with its last repeat region
- DC-SIGNR, in addition to DC-SIGN, should be considered as a cofactor in sexual HIV-1 transmission; soluble isoforms, in particular, may modulate the efficiency of viral transmission and dissemination.
- A change in the number of DC-SIGN-related (DC-SIGNR) repeats may influence its normal functions as well as its binding capacity to viral and nonviral pathogens.
- genetic risk association study shows that individuals homozygous for CLEC4M tandem repeats are less susceptible to SARS infection.
- Recognizes pathogens and contributes to innate immunity.
- Molecular explanation for the ability of DC-SIGN-interacting pathogens to preferentially evoke Th2-type immune responses.
- DC-SIGNR homozygous 7/7 repeat was associated with increased risk of HIV-1 infection; the heterozygous 7/5 repeat tended to be correlated with resistance to HIV-1 infection; findings suggest DC-SIGNR polymorphisms may influence susceptibility to HIV-1
- DC-SIGN and DC-SIGNR genotypes and alleles distribution in Chinese Han population are significantly different from Caucasian population and with Chinese own population genetic characteristics, compared with Caucasians.
- molecular mechanism whereby L-SIGN polymorphism could influence the establishment and progression of HCV infection
- Expression of VAP-1, Stabilin-1, L-SIGN can be used to identify sinusoidal endothelium and to permit discrimination from vascular and lymphatic endothelial cells.
- CD209L (L-SIGN) is used by coronavirus 229E to enter cells
- study showed that polymorphisms in DC-SIGNR were significantly associated with HIV-exposed seronegative (ESN) Thai females but not with ESN males
- DC-SIGN- and L-SIGN-mediated severe acute respiratory syndrome coronavirus entry requires specific asparagine-linked glycosylation sites
- A total of 13 genotypes were found in DC-SIGNR neck repeat region polymorphism. Among all the genotypes, only 5/5 homozygous showed significant reduced risk of HIV-1 infection in HIV-1-exposed seronegative individuals
- Our results reveal important differences between Ebola virus and HIV-1 capture by DC-SIGN/R and LSECtin and hint towards different biological functions of these lectins.
- when B-ALL cells enter the blood circulation and are able to interact with DC-SIGN and L-SIGN the immune response is shifted toward tolerance.
- DC-SIGN, DC-SIGNR and SDF-1 polymorphism was detected in high risk seronegative and HIV-1 patients in Northern Asian Indians.
- association of the polymorphism of homologue of dendritic cell-specific intercellular adhesion molecule-3 (ICAM-3) grabbing nonintegrin (DC-SIGN related, DC-SIGNR) gene with the susceptibility to HIV-1 infection