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TUNEL Chromogenic Apoptosis Detection Kit

Introduction

Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment.

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Hapten-tagged dUTP (e.g. digoxigenin-dUTP or biotin-dUTP) can be detected using secondary reagents (e.g. anti-digoxigenin antibodies or streptavi­din) for fluorescence or colorimetric detection.

Alternatively, fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry. The TUNEL Chromogenic Apoptosis Detection Kit contains dUTP conjugated to biotin and HRP-Streptavidin conjugate for high sensitive TUNEL staining.

Features

  • Broad application: Suitable for FFPE tissue, frozen tissue and cells.
  • High sensitivity:Can detect a low population of apoptotic cells.

Specifications

Platform: Light Microscopy
Detection Method: Colormetric
Ex/Em:
Product Size: 50 assays
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Dry ice

Applications

Cell apoptosis analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Figure 1. Detection of apoptotic cells in mouse tongue tissue using  TUNEL Chromogenic Apoptosis Detection Kit.

To order

Buy Cat.No. Product Name Ex/Em Unit Price
A049 TUNEL Chromogenic Apoptosis Detection Kit 50 assay $380

 
   

Documents

Protocol (PDF): A049
   

References:

Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.
Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H,
Genes Cells (2010) 15:315-325

Vasopressin inhibits apoptosis in renal collecting duct cells.

 

 

 

 

 

Miller RL, Sandoval PC, Pisitkun T, Knepper MA, Hoffert JD,
Am J Physiol Renal Physiol (2013) 304:F177-F188