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TUNEL Andy Fluor™ 488 Apoptosis Detection Kit

Introduction

Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment.

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Hapten-tagged dUTP (e.g. digoxigenin-dUTP or biotin-dUTP) can be detected using secondary reagents (e.g. anti-digoxigenin antibodies or streptavi­din) for fluorescence or colorimetric detection.

 

Alternatively, fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry. The TUNEL Andy Fluor™ 488 Apoptosis Detection Kit contains dUTP conjugated to biotin and Streptavidin conjugated to bright and photostable Andy Fluor™ 488 green fluores­cent dye, for bright fluorescent TUNEL staining.

Features

  • Broad application: Suitable for FFPE tissue, frozen tissue and cells.
  • High sensitivity: Can detect a low population of apoptotic cells.
  • Bright apoptotic signal: Uses Andy Fluor dyes, resulting in a stable, non-photobleaching fluorescent signal.

Specifications

Platform: Fluorescence Microscopy
Detection Method: Fluorescent
Ex/Em: 495/520 nm
Product Size: 50 assays
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Dry ice

Applications

Cell apoptosis analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Figure 1. Detection of apoptotic cells in mouse tongue tissue using  TUNEL Andy Fluor™ 488 Apoptosis Detection Kit.

To order

Buy Cat.No. Product Name Ex/Em Unit Price
A050 TUNEL Andy Fluor™ 488 Apoptosis Detection Kit 495/520 50 assay $380

 
   

Documents

Protocol (PDF): A050
   
MSDS (PDF): A050

 

References:

Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.
Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H,
Genes Cells (2010) 15:315-325

 

 

 

 

 

 

Vasopressin inhibits apoptosis in renal collecting duct cells.
Miller RL, Sandoval PC, Pisitkun T, Knepper MA, Hoffert JD,
Am J Physiol Renal Physiol (2013) 304:F177-F188