Select TALEN / CRISPR-Cas9 Expression Clones [Search again]

Product ID: RTN250037(click here to view gene annotation page) (click here to view gene annotation page)
Symbol: Cacna1e
Species: Rat
Target Gene Accession: NM_019294.2
Alias: CACHA1E, Cav2.3
Gene Description: calcium voltage-gated channel subunit alpha1 E
Delivery format: 10 μg purified plasmid
Estimated Delivery:  Approximately 2-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.

Important Note: By default, the TALEN/CRISPR constructs are designed to target the protein coding-region as closely as possible to the initiator ATG of the splice variant (accession number) you selected. This design strategy does not consider other possible variants of this gene. If this gene has multiple variants and you would like to target one particular exon, one unique variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
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25% off Selected ORF Clones
20% off shRNA clones
20% off Custom Lentivirus
20% off Custom AAV

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  • CRISPR
  • TALEN
  • HDR Donor Clones
  • HDR Donor Vectors
  • Indel Detection
  • Lentiviral Particles
  • Complete Solutions
The CRISPR sgRNA clone options listed below are intended for use as 20 nt sgRNAs with the wild-type Cas9 nuclease from Streptococcus pyogenes (SpCas9). GeneCopoeia does provide alternate CRISPR options, such as Cas9 D10A nickase and other high-fidelity versions of Cas9, SaCas9, and shorter sgRNAs. To purchase reagents for these other options, please contact us for a custom quote.

Check out our Cas9 Stable Cell Lines!

Buy Catalog# Description Number of target sites Selection marker Reporter gene Vector
Non-viral clones
CP-C9NU-01 Cas9 nuclease expression clone (Purified plasmid) 1 Neo mCherry CP-C9NU-01
RCP250037-SG01-1 1 × sgRNA expression clone targeting Cacna1e (NM_019294.2) 1 Hygro N/A pCRISPR-SG01
RCP250037-SG01-3 3 × sgRNA expression clones targeting Cacna1e (NM_019294.2) 3 Hygro N/A pCRISPR-SG01
All-in-one non-viral clones
RCP250037-CG02-1 1 × sgRNA/Cas9 all-in-one expression clone targeting Cacna1e (NM_019294.2) 1 N/A N/A pCRISPR-CG02
RCP250037-CG02-3 3 × sgRNA/Cas9 all-in-one expression clones targeting Cacna1e (NM_019294.2) 3 N/A N/A pCRISPR-CG02
RCP250037-CG04-1 1 × sgRNA/Cas9 all-in-one expression clone targeting Cacna1e (NM_019294.2) 1 Neo copGFP pCRISPR-CG04
RCP250037-CG04-3 3 × sgRNA/Cas9 all-in-one expression clones targeting Cacna1e (NM_019294.2) 3 Neo copGFP pCRISPR-CG04
RCP250037-CG07-1 1 × sgRNA/Cas9 all-in-one expression clone targeting Cacna1e (NM_019294.2) 1 N/A copGFP pCRISPR-CG07
RCP250037-CG07-3 3 × sgRNA/Cas9 all-in-one expression clones targeting Cacna1e (NM_019294.2) 3 N/A copGFP pCRISPR-CG07
RCP250037-CG08-1 1 × sgRNA/Cas9 all-in-one expression clone targeting Cacna1e (NM_019294.2) 1 N/A mCherry pCRISPR-CG08
RCP250037-CG08-3 3 × sgRNA/Cas9 all-in-one expression clones targeting Cacna1e (NM_019294.2) 3 N/A mCherry pCRISPR-CG08
RCP250037-CG12-1 1 × sgRNA/Cas9 all-in-one expression clone targeting Cacna1e (NM_019294.2) 1 Neo mCherry pCRISPR-CG12
RCP250037-CG12-3 3 × sgRNA/Cas9 all-in-one expression clones targeting Cacna1e (NM_019294.2) 3 Neo mCherry pCRISPR-CG12
Lentiviral clones (packaged particles can be found under Lentiviral particle tab)
CP-LvC9NU-01 Cas9 nuclease lentiviral expression clone (-CMV promoter) (Purified plasmid) 1 Neo N/A CP-LvC9NU-01
CP-LvC9NU-02 Cas9 nuclease lentiviral expression clone (-CMV promoter) (Purified plasmid) 1 Neo eGFP CP-LvC9NU-02
CP-LvC9NU-08 Cas9 nuclease lentiviral expression clone (-EF1a promoter) (Purified plasmid) 1 Puro N/A CP-LvC9NU-08
CP-LvC9NU-09 Cas9 nuclease lentiviral expression clone (-EF1a promoter) (Purified plasmid) 1 Neo eGFP CP-LvC9NU-09
CP-LvC9NU-10 Cas9 nuclease lentiviral expression clone (-EF1a promoter) (Purified plasmid) 1 Hygro eGFP CP-LvC9NU-10
RCP250037-LvSG03-1 1×sgRNA lentiviral expression clone targeting Cacna1e (NM_019294.2) 1 Puro mCherry pCRISPR-LvSG03
RCP250037-LvSG03-3 3×sgRNA lentiviral expression clones targeting Cacna1e (NM_019294.2) 3 Puro mCherry pCRISPR-LvSG03

For species other than human/mouse or for custom orders, click CRISPR

Warranty Statements


TALEN clones

Buy Catalog# Description Number of target sites Selection marker Reporter gene Vector
RTN250037-1 1 × pair of TALEN expression clones targeting Cacna1e (NM_019294.2) 1 Hygro N/A pTALEN-01
RTN250037-2 2 × pairs of TALEN expression clones targeting Cacna1e (NM_019294.2) 2 Hygro N/A pTALEN-01
RTN250037-3 3 × pairs of TALEN expression clones targeting Cacna1e (NM_019294.2) 3 Hygro N/A pTALEN-01

For species other than human/mouse or for custom order, click for TALEN .

Warranty Statements


HDR Donor Clones

Homology-dependent repair (HDR) gene knockout donors are designed to knock out your gene of interest using GeneCopoeia CRISPR or TALEN clones. Choose a vector backbone for your gene-specific HDR knockout clone from the list below. For other HDR donor applications, including conditional knockout, mutagenesis, in-frame fusion tagging, and more, please contact us for a custom quote.

Buy Catalog# Description Vector Promoter Reporter Gene Selection Marker LoxP site
DC-RTN250037-D01 Genome-Editing donor clone (copGFP/Puromycin) for knockout of Cacna1e (NM_019294.2) pDonor-D01 EFa1 copGFP Puromycin N/A
DC-RTN250037-D02 Genome-Editing donor clone (copGFP/Neomycin) for knockout of Cacna1e (NM_019294.2) pDonor-D02 CMV copGFP Neomycin N/A
DC-RTN250037-D03 Genome-Editing donor clone (Neomycin) for knockout of Cacna1e (NM_019294.2) pDonor-D03 CMV N/A Neomycin N/A
DC-RTN250037-D04 Genome-Editing donor clone (Puromycin) for knockout of Cacna1e (NM_019294.2) pDonor-D04 CMV N/A Puromycin N/A
DC-RTN250037-D05 Genome-Editing donor clone (Neomycin) for knockout of Cacna1e (NM_019294.2) pDonor-D05 EFa1 N/A Neomycin N/A
DC-RTN250037-D07 Genome-Editing donor clone (copGFP/Puromycin/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D07 EFa1 copGFP Puromycin/TK LoxP
DC-RTN250037-D08 Genome-Editing donor clone (copGFP/Neomycin/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D08 CMV copGFP Neomycin/TK LoxP
DC-RTN250037-D09 Genome-Editing donor clone (Puromycin/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D09 EFa1 N/A Puromycin/TK LoxP
DC-RTN250037-D10 Genome-Editing donor clone (Neomycin/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D10 CMV N/A Neomycin/TK LoxP
DC-RTN250037-D11 Genome-Editing donor clone (PGK/copGFP/Puro/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D11 PGK copGFP Puromycin LoxP
DC-RTN250037-D12 Genome-Editing donor clone (EF1a/copGFP/Hygro/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D12 EF1a copGFP Hygromycin/TK LoxP
DC-RTN250037-D13 Genome-Editing donor clone (PGK/copGFP/Neo/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D13 PGK copGFP Neomycin/TK LoxP
DC-RTN250037-D14 Genome-Editing donor clone (PGK/Puro/TK/LoxP) for knockout of Cacna1e (NM_019294.2) pDonor-D14 PGK N/A Puromycin/TK LoxP

Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more. Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more. Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more.

HDR Donor Vectors

Buy Vector Description Promoter Reporter Gene Selection Marker LoxP site
pDonor-D01 Genome-Editing Donor cloning vector (copGFP/Puromycin) EFa1 copGFP Puromycin N/A
pDonor-D02 Genome-Editing Donor cloning vector (copGFP/Neomycin) CMV copGFP Neomycin N/A
pDonor-D03 Genome-Editing Donor cloning vector (Neomycin) CMV N/A Neomycin N/A
pDonor-D04 Genome-Editing Donor cloning vector (Puromycin) CMV N/A Puromycin N/A
pDonor-D05 Genome-Editing Donor cloning vector (Neomycin) EFa1 N/A Neomycin N/A
pDonor-D07 Genome-Editing Donor cloning vector (copGFP/Puromycin/TK/LoxP) EFa1 copGFP Puromycin/TK LoxP
pDonor-D08 Genome-Editing Donor cloning vector (copGFP/Neomycin/TK/LoxP) CMV copGFP Neomycin/TK LoxP
pDonor-D09 Genome-Editing Donor cloning vector (Puromycin/TK/LoxP) EFa1 N/A Puromycin/TK LoxP
pDonor-D10 Genome-Editing Donor cloning vector (Neomycin/TK/LoxP) CMV N/A Neomycin/TK LoxP
pDonor-D11 Genome-Editing Donor cloning vector (PGK/copGFP/Puro/TK/LoxP) PGK copGFP Puromycin/TK LoxP
pDonor-D12 Genome-Editing Donor cloning vector (EF1a/copGFP/Hygro/TK/LoxP) EF1a copGFP Hygromycin/TK LoxP
pDonor-D13 Genome-Editing Donor cloning vector (PGK/copGFP/Neo/TK/LoxP) PGK copGFP Neomycin/TK LoxP
pDonor-D14 Genome-Editing Donor cloning vector (PGK/Puro/TK/LoxP) PGK N/A Puromycin/TK LoxP

Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more.



IndelCheck™ CRISPR/TALEN insertion or deletion (indel) detection system (version 2.0)

Buy Catalog Product Description Price
IC001 IndelCheck™ CRISPR/TALEN insertion or deletion detection system (50 rxns) Includes target site PCR kit (IC003) and T7 endonuclease I assay kit (IC005) inquire
IC002 IndelCheck™ CRISPR/TALEN insertion or deletion detection system (200 rxns) Includes target site PCR kit (IC004) and T7 endonuclease I assay kit (IC006) inquire
IC003 Target site PCR Kit (version 2.0) , 50 rxns PCR reagents for amplifying region flanking CRISPR/TALEN target site, prior to T7 Endonuclease I digestion* inquire
IC004 Target site PCR Kit (version 2.0), 200 rxns PCR reagents for amplifying region flanking CRISPR/TALEN target site, prior to T7 Endonuclease I digestion* inquire
IC005 T7 endonuclease I assay kit, 50 rxns Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations inquire
IC006 T7 endonuclease I assay kit, 200 rxns Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations inquire
IC007 Target site PCR cloning kit, 20 rxns Clone PCR products for sequencing and identification of CRISPR- or TALEN-mediated genomic modifications Coming Soon
IC008 Target site PCR cloning kit, 100 rxns Clone PCR products for sequencing and identification of CRISPR- or TALEN-mediated genomic modifications Coming Soon

Target PCR primer pair

Buy Catalog# Product Target site
Target site PCR primer
(designed to only amplify the genomic DNA region including the corresponding genecopoeia CRISPR target site(s))
PRCP250037-a Target site PCR primer pair for indel detection (target site 1)(200 rxn) Target site 1
PRCP250037-b Target site PCR primer pair for indel detection (target site 2)(200 rxn) Target site 2
PRCP250037-c Target site PCR primer pair for indel detection (target site 3)(200 rxn) Target site 3
Target site PCR primer for nickase pair
(designed to only amplify the genomic DNA region including the corresponding genecopoeia CRISPR target site(s))
PRCP250037-2N Target site PCR primer for indel detection (nickase pair target site)(200 rxn) Nickase pair target site

Chromosomal validation

Buy Catalog# Product Target site
CRISPR Chromosomal validation service
C-RCP250037-a Chromosomal validation service (target site 1) Target site 1
C-RCP250037-b Chromosomal validation service (target site 2) Target site 2
C-RCP250037-c Chromosomal validation service (target site 3) Target site 3
TALEN Chromosomal validation service
C-RTN250037-a Chromosomal validation service (target site 1) Target site 1
C-RTN250037-b Chromosomal validation service (target site 2) Target site 2
C-RTN250037-c Chromosomal validation service (target site 3) Target site 3

Lentiviral Particles

Check out our Cas9 Stable Cell Lines!
Buy Catalog# Product Description # of sgRNAs Delivery Vector
Cas9
LPP-CP-LvC9NU-01-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles >1×106 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µL.
N/A 100 µL×1 vial CP-LvC9NU-01
LPP-CP-LvC9NU-02-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles >1×106 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µL. N/A 100 µL×1 vial CP-LvC9NU-02
LPP-CP-LvC9NU-08-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µL x 1 vial)
 >1×107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µL. N/A 100 µL×1 vial CP-LvC9NU-08
LPP-CP-LvC9NU-09-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µL x 1 vial)
 >1×107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µL. N/A 100 µL×1 vial CP-LvC9NU-09
LPP-CP-LvC9NU-10-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µL x 1 vial)
 >1×107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µL. N/A 100 µL×1 vial CP-LvC9NU-10
sgRNA control particles
LPPCCPCTR01L03-025 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 25 µl (U6/T7/mCherry/Puro) 1 25 µL×1 vial pCRISPR-LvSG03
LPPCCPCTR01L03-100 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 100 µl (U6/T7/mCherry/Puro) 1 25 µL×4 vial pCRISPR-LvSG03
sgRNA (1 target site)
LPPRCP250037L03-1-50 Purified Lentifect™ Lentiviral Particles targeting Cacna1e (NM_019294.2) >1×108 TU/ml sgRNA lentiviral particles ready for transduction (U6/T7/mCherry/Puro) 1 50 µL×1 vial pCRISPR-LvSG03
LPPRCP250037L03-1-100 Purified Lentifect™ Lentiviral Particles targeting Cacna1e (NM_019294.2) >1×108 TU/ml sgRNA lentiviral particles ready for transduction (U6/T7/mCherry/Puro) 1 100 µL×1 vial pCRISPR-LvSG03
LPPRCP250037L03-1-200 Purified Lentifect™ Lentiviral Particles targeting Cacna1e (NM_019294.2) >1×108 TU/ml sgRNA lentiviral particles ready for transduction (U6/T7/mCherry/Puro) 1 200 µL×1 vial pCRISPR-LvSG03
sgRNA (3 target site)
LPPRCP250037L03-3-50 Purified Lentifect™ Lentiviral Particles targeting Cacna1e (NM_019294.2) >1×108 TU/ml sgRNA lentiviral particles ready for transduction (U6/T7/mCherry/Puro) 3 50 µL×1 vial pCRISPR-LvSG03
LPPRCP250037L03-3-100 Purified Lentifect™ Lentiviral Particles targeting Cacna1e (NM_019294.2) >1×108 TU/ml sgRNA lentiviral particles ready for transduction (U6/T7/mCherry/Puro) 3 100 µL×1 vial pCRISPR-LvSG03
LPPRCP250037L03-3-200 Purified Lentifect™ Lentiviral Particles targeting Cacna1e (NM_019294.2) >1×108 TU/ml sgRNA lentiviral particles ready for transduction (U6/T7/mCherry/Puro) 3 200 µL×1 vial pCRISPR-LvSG03

 


Additional services

Safe Harbor Knockin

Buy Catalog# Product Description Price
Human AAVS1 safe harbor knockin
SH029 Genome-TALER™ human AAVS1 safe harbor gene knock-in kit Includes:
AAVS1 TALEN pair (TN-AAVS1)
AAVS1 donor cloning vector (DC-DON-SH01)
AAVS1 positive control donor (DC-RFP-SH01)
knock-in verification primer pairs (HQPAVSHR)		 inquire
SH025 Genome-TALER™ human AAVS1 safe harbor gene knock-in kit
(without donor)		 Includes: 
AAVS1 TALEN pair (TN-AAVS1)
AAVS1 positive control (DC-RFP-SH01)
knock-in verification primer pairs
(HQPAVSHR)		 inquire
SH004 Genome-CRISP™ human AAVS1 safe harbor gene knock-in kit Includes:
AAVS1 All-in-one sgRNA/Cas9 expression clone (HCP-AAVS1-CG02)
AAVS1 donor cloning vector (DC-DON-SH01)
AAVS1 positive control donor (DC-RFP-SH01)
knock-in verification primer pairs (HQPAVSHR)		 inquire
SH000 Genome-CRISP™ human AAVS1 safe harbor gene knock-in kit
(without donor)		 Includes:
AAVS1 All-in-one sgRNA/Cas9 expression clone (HCP-AAVS1-CG02)
AAVS1 positive control donor (DC-RFP-SH01)
knock-in verification primer pairs (HQPAVSHR)		 inquire
Mouse ROSA26 safe harbor knockin
SH079 Genome-TALER™ 
mouse ROSA26 safe harbor gene knock-in kit		 Includes:
ROSA26 TALEN pair (TN-ROSA26)
ROSA26 donor cloning vector (DC-DON-SH02)
ROSA26 positive control donor (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)		 inquire
SH075 Genome-TALER™ 
mouse ROSA26 safe harbor gene knock-in kit
(without donor)		 Includes:
ROSA26 TALEN pair (TN-ROSA26)
ROSA26 positive control (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)		 inquire
SH054 Genome-CRISP™ 
mouse ROSA26 safe harbor gene knock-in kit		 Includes:
ROSA26 sgRNA/Cas9 expression clone (MCP-ROSA26-CG01)
ROSA26 donor cloning vector (DC-DON-SH02)
ROSA26 positive control donor (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)		 inquire
SH050 Genome-CRISP™
mouse ROSA26 safe harbor gene knock-in kit
(without donor)		 Includes:
ROSA26 sgRNA/Cas9 expression clone (MCP-ROSA26-CG01)
ROSA26 positive control (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)		 inquire
Cas9 safe harbor knockin clones
SH300 human AAVS1 safe harbor Cas9 knock-in clone AAVS1 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into human AAVS1 safe harbor locus. inquire
SH301 human AAVS1 safe harbor Cas9 knock-in clone AAVS1 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into human AAVS1 safe harbor locus. (Hygromycin) inquire
SH302 human AAVS1 safe harbor Cas9 knock-in clone AAVS1 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into human AAVS1 safe harbor locus. (Neomycin) inquire
SH350 mouse ROSA26 safe harbor Cas9 knock-in clone ROSA26 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into mouse ROSA26 safe harbor locus. (Puromycin) inquire
SH351 mouse ROSA26 safe harbor Cas9 knock-in clone ROSA26 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into mouse ROSA26 safe harbor locus. (Hygromycin) inquire
SH352 mouse ROSA26 safe harbor Cas9 knock-in clone ROSA26 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into mouse ROSA26 safe harbor locus. (Neomycin) inquire
Variable AAVS1 knock-in ORF donor clone AAVS1 knock-in ORF donor clone containing CMV-driven ORF of customer's gene of interest


Related products

For TALEN/CRISPR-mediated human and mouse safe harbor site knockin, GeneCopoeia offers Safe Harbor knockin kits and Safe Harbor knockin human ORF clones.