Validated All-in-One™ qPCR Primer for DHCR7(NM_001360.2) [Search again]

Catalog#: HQP004307(click here to view gene annotation page)
Symbol: DHCR7
Species: Human
Target Gene Accession: NM_001360.2
Alias: SLOS
Gene Description: 7-dehydrocholesterol reductase
Estimated Delivery: 1-3 weeks, but may vary. Please email sales@genecopoeia.com  to confirm ETA.
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Important Note: By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.


Buy Catalog# Description Note Price
HQP004307 All-in-One™ qPCR Primer for NM_001360.2 (200 reactions) DHCR7 inquire
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HQP015171 All-in-One™ qPCR Primer for NM_004048.3 (200 reactions) Reference Gene B2M inquire
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Validated result:

AMP Melt
AB
Figure: All-in-One™ qPCR Primer Validated result to DHCR7.

Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM].


Gene References into function