Validated All-in-One™ qPCR Primer for TPP1(NM_000391.3) [Search again]

Catalog#: HQP002078(click here to view gene annotation page)
Symbol: TPP1
Species: Human
Target Gene Accession: NM_000391.3
Alias: CLN2, GIG1, LPIC, SCAR7, TPP-1
Gene Description: tripeptidyl peptidase 1
Estimated Delivery: 1-3 weeks, but may vary. Please email sales@genecopoeia.com  to confirm ETA.
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Important Note: By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.


Buy Catalog# Description Note Price
HQP002078 All-in-One™ qPCR Primer for NM_000391.3 (200 reactions) TPP1 inquire
HQP006940 All-in-One™ qPCR Primer for NM_002046.6 (200 reactions) Reference Gene GAPDH inquire
HQP015171 All-in-One™ qPCR Primer for NM_004048.3 (200 reactions) Reference Gene B2M inquire
HQP016381 All-in-One™ qPCR Primer for NM_001101.4 (200 reactions) Reference Gene ACTB inquire


Validated result:

AMP Melt
AB
Figure: All-in-One™ qPCR Primer Validated result to TPP1.

Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq].


Gene References into function