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Protein Detection and Quantitation

Introduction Protein gel staining and Western Blotting are important tools in proteomics (Figure 1). GeneCopoeia offers two distinct protein gel stains: 1) Coomassie® Blue Fast Stain, a ready-to-use product that does not require methanol or acetic acid fixatives or destains; 2) eLuminol™ Protein Gel Stain, a highly-sensitive fluorescent stain designed to detect proteins in SDS polyacrylamide gels.  GeneCopoeia’ ECL Western Blotting Detection Kit provides highly sensitive detection on immunoblots. Besides, GeneCopoeia also offers two protein assay kits: 1) Bradford Protein Assay Kit; and 2) BCA Protein Assay Kit.

Figure 1. Protein gel analysis workflow

Protein Gel Stain Selection Guide

Coomassie® Blue Fast Stain eLuminol™ Protein Gel Stain
Sensitivity 20 ng  0.5 ng
Ease of use Ready to use Supplied as stock solution
Time required 2 hr (standard) 20 min (microwave) 90 min (standard) 30 min (microwave)
Detection method colorimetric fluorescent
MS spec. compatible       Yes Yes
 
Coomassie
Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.
ECL Western Blotting Detection Kit
Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).
                                      Bradford Protein Assay Kit  BCA Protein Assay Kit

Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).  
To order
Buy Catalog # Product Name Description Size List Price
P001 Coomassie® Blue Fast Stain Colorimetric protein gel stain 1 L $70
P003A P003B eLuminol™ Protein Gel Stain (1,000X) Fluorescent protein gel stain 500 µL 1 mL $70 $120
P007 ECL Western Blotting Detection Kit Western blotting reagent 100 mL $80
P010 Bradford Protein Assay Kit Protein quantitation 500 mL $75
P011 BCA Protein Assay Kit Protein quantitation 500 mL $95
P013 SDS-PAGE Sample Loading Buffer (5X) Loading buffer 5 mL $20
 
FAQs

Frequently Asked Questions

Answer: eLuminol™ has two excitation wavelengths: 300 nm and 460 nm. The emission wavelength is around 600 nm.
Publications 2018