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Nucleic Acid Quantitation

Introduction

GeneCopoeia's iQuant™ DNA or RNA Quantitation Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our new iQuant™ NGS dsDNA assay kits are ideal for quantitating DNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides  more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.

Features

  • Higher sensitivity: 100 times more sensitive than NanoDrop;
  • Broad dynamic range: over four orders of magnitude;
  • More accuracy: unaffected by the presence of proteins, free nucleotides;
  • Easy to use: mix-and-read format;
  • Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit® or Quantus™.

DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).


Figure 1. Nucleic acid research workflow

GeneCopoeia's iQuant™ NGS dsDNA assay kits provide a very simple workflow (Figure 2).


Figure 2. iQuant™ NGS dsDNA assay workflow

In addition, GeneCopoeia's iQuant™ DNA Quantitation Kits provide higher sensitivity than absorbance-based quantitation methods, such as NanoDrop (Figure 3).

                            
Figure 3. Improved sensitivity with iQuant™ DNA Quantitation Kits

Further, iQuant™ DNA Quantitation Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 4).


Figure 4. Selectivity for dsDNA using iQuant™ HS dsDNA Quantitation Kit in the presence of an equal mass of RNA.

iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with NanoDrop, especially at relatively low DNA concentrations (Figure 5).


Figure 5. Comparison of accuracy of DNA quantitation between the iQuant™ HS dsDNA Quantitation Kit and NanoDrop.

The iQuant™ DNA kits for DNA quantitation are simple to use. Just mix your DNA sample with iQuant™ dye solution, and perform the assays in either a multi-well plate reader, or in single tubes (Figure 6). The iQuant™ kits are compatible with the Qubit® fluorometer from Life Technologies and the Quantus™ fluorometer from Promega.


Figure 6. Assay workflow for iQuant™ DNA Quantitation Kits