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Monobromobimane (mBBr)

Introduction

Monobromobimane (mBBr) is essentially nonfluorescent, and freely passively diffuse across the plasma membrane into the cytoplasm, where they form blue-fluorescent adducts with intracellular glutathione and thiol containing proteins. The glutathione conjugate of monobromobimane has absorption/emission maxima ~394/490 nm. mBBr has been reported to be used as effective tracer of gap-junctional communication.

Specifications:

 

  

Excitation/Emission:

385/463 nm

Shipping Condition:

Ambient

Storage Conditions:

-20ºC, protect from light

Molecular Formula:

C10H11BrN2O2

Molecular Weight:

271.11

CAS Number:

71418-44-5

 

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C038 Monobromobimane (mBBr) 385/463 25 mg $100

Protocol
MSDS
COA

Documents

Protocol (PDF): C038
MSDS (PDF):  MSDS-C038
COA (PDF):  COA-C038

Reference:

Formation of S-nitrosothiols via direct nucleophilic nitrosation of thiols by peroxynitrite with elimination of hydrogen peroxide.
van der Vliet A, Hoen PA, Wong PS, Bast A, Cross CE
J Biol Chem (1998) 273:30255-30262

Orientation of actin monomer in the F-actin filament: radial coordinate of glutamine-41 and effect of myosin subfragment 1 binding on the monomer orientation.
Kasprzak AA, Takashi R, Morales MF
Biochemistry (1988) 27:4512-4522

Structure of actin observed by fluorescence resonance energy transfer spectroscopy.
Miki M, O’Donoghue SI, Dos Remedios CG
J Muscle Res Cell Motil (1992) 13:132-145