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JC-1 Mitochondrial Membrane Potential Detection Kit

Introduction

JC-1 Mitochondrial Membrane Potential Detection Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry. The loss of mitochondrial membrane potential is a hallmark for apoptosis. The JC-1 Assay Kit uses a unique cationic dye (5,5’,6,6’- tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide) to signal the loss of mitochondrial membrane potential. JC-1 exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (529 nm) to red (590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. In healthy cells, the JC-1 accumulates in the mitochondrial to form J-aggregates, which become red fluorescence. In apoptotic cells, the JC-1 cannot accumulate within the mitochondria and remains in the cytoplasm in monomeric form to show green fluorescence.    

 

Features

  • Simple workflow: add, incubate, and read format, no wash needed.
  • Flexibility: suitable for flow cytometry, fluorescence microscope, and plate reader.
  • Fast: results in as little as 30 minutes.

Specifications

Platform: Fluorescence Microscopy, Flow Cytometry, Plate Reader
Detection Method: Fluorescent
Ex/Em: 514/529, 590 nm
Product Size: 100 assay
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications

Cell apoptosis analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Figure 1. Detection of cell apoptosis with JC-1 Mitochondrial Membrane Potential Detection Kit. Jurkat cells treated with 10 μM camptothecin for 4 hr (right panel) or untreated (left panel). Cells were stained with JC-1 reagent, then analyzed by flow cytometry.

 

To order

Buy Cat.No. Product Name Ex/Em Unit Price
A048 JC-1 Mitochondrial Membrane Potential Detection Kit 514/529,590 100 assay $180

Protocol
   

Documents

Protocol (PDF): A048
MSDS (PDF):  MSDS-A048

References:

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Mol Cancer Ther (2011) 10:1276-1288

Tissue transglutaminase is a multifunctional BH3-only protein.
Rodolfo C, Mormone E, Matarrese P, Ciccosanti F, Farrace MG, Garofano E, Piredda L, Fimia GM, Malorni W, Piacentini M
J Biol Chem (2004) 279:54783-54792

High-throughput screening for daunorubicin-mediated drug resistance identifies mometasone furoate as a novel ABCB1-reversal agent.
Winter SS, Lovato DM, Khawaja HM, Edwards BS, Steele ID, Young SM, Oprea TI, Sklar LA, Larson RS,
J Biomol Screen (2008) 13:185-193

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