You are here: Home > Products >

iQuant™ Broad Range RNA Quantitation Kit

Introduction

The iQuant™ Broad Range RNA Quantitation Kit is designed specifically for an easy and accurate quantitation of RNA. The kit is highly selective for RNA in the prescent of DNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 50 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.


Figure 1. Left: Quantitation of ribosomal RNA with iQuant™ BR RNA Quantitation Kit using fluorescence plate reader. Right: Quantitation of ribosomal RNA with iQuant™ BR RNA Quantitation Kit using Qubit® Fluorometer.

Specifications:

Sample Type: RNA
Platform: Fluorescence Plate Reader or Fluorometer
Linear Range: 20-1000 ng RNA

Applications

RNA quatitation in solution (Figure 2).


Figure 2. iQuant™ BR RNA quantitation assay workflow