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iQuant™ Broad Range dsDNA Quantitation Kit

Introduction

The iQuant™ Broad Range dsDNA Quantitation Kit is designed specifically for an easy and accurate quantitation of dsDNA. The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted dsDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 50 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.

Figure 1. Left: Quantitation of plasmid dsDNA with iQuant™ BR dsDNA Quantitation Kit using fluorescence plate reader. Right: Quantitation of plasmid dsDNA with iQuant™ BR dsDNA Quantitation Kit using Qubit® Fluorometer.

Specifications:

Sample Type: dsDNA
Platform: Fluorescence Plate Reader or Fluorometer
Linear Range: 2-1000 ng dsDNA

Applications

dsDNA quatitation in solution (Figure 2).

Figure 2. iQuant™ BR dsDNA quantitation assay workflow