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Hoechst 33342


Hoechst 33342 is a popular cell membrane-permeant nuclear counterstain. Hoechst 33342 emits blue fluorescence upon binding to the minor groove of dsDNA. Hoechst 33342 are widely used for nuclear counterstaining, apoptosis and cell cycle studies. Hoechst 33342 is provided as a 10 mg solid (C005) and a 10 mg/mL aqueous solution (C006).

Excitation/Emission: 350/461 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C27H37Cl3N6O4
Molecular Weight: 615.99
CAS Number: 23491-52-3


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price

Hoechst 33342 350/461 10 mg
1 ml





Protocol (PDF): C005  C006
COA (PDF):  C005 C006


Mgat5 and Pten interact to regulate cell growth and polarity.
Cheung P, Dennis JW,
Glycobiology (2007) 17:767-773
Product usage: Oncology, Cell Signaling, Transcription Factors, Cell Cycle, Cell Proliferation, HCS, ArrayScan, Nuclear stain

Identification of a novel function for the chromatin remodeling protein ING2 in muscle differentiation.
Eapen SA, Netherton SJ, Sarker KP, Deng L, Chan A, Riabowol K, Bonni S,
PLoS One (2012) 7:e40684-e40684
Product usage: Oncology, Cell Signaling, Transcription factors, Cellomics,Cell Proliferation, Cell cycle, Nuclear stain, C2C12 cells, Cell Signaling, Transcription factors

High-content screening with siRNA optimizes a cell biological approach to drug discovery: defining the role of P53 activation in the cellular response to anticancer drugs.
Giuliano KA, Chen YT, Taylor DL,
J Biomol Screen (2004) 9:557-568

Frequently Asked Questions (FAQs)

I have a mix of live bacteria and platelet cells, and I need to be able to separate out the bacteria. Do you have a suggestion?
Platelet cells don’t have a nucleus and thus lack nuclear DNA. Therefore, a cell-permeant, DNA-selective dye would label bacteria with greater intensity relative to minor staining of the platelet mitochondrial DNA. We recommend using a Hoechst nucleic acid stain.

I need a total cell stain to label cytoplasm and nuclei in live cells. What do you recommend?
You can use a combination of two dyes with overlapping blue emission. For cytoplasm, you can label the cell with CellView Blue CMAC. This can be combined with Hoechst 33342 for nuclei. Both dyes are imaged using a standard DAPI filter set.