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Hoechst 33258


Hoechst 33258 is a popular cell membrane-permeant nuclear counterstain. Hoechst 33258 emits blue fluorescence upon binding to the minor groove of dsDNA. Hoechst 33258 are widely used for nuclear counterstaining, apoptosis and cell cycle studies. Hoechst 33258 is provided as a 10 mg solid (C003) and a 10 mg/mL aqueous solution (C004).

Excitation/Emission: 352/461 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C25H37Cl3N6O6
Molecular Weight: 623.96
CAS Number: 23491-45-4  


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price

Hoechst 33258 352/461 10 mg
1 ml





Protocol (PDF): C003 C004
MSDS (PDF):  C003 C004
COA (PDF):  C003 C004


Synthetic lethality of cohesins with PARPs and replication fork mediators.
McLellan JL, O'Neil NJ, Barrett I, Ferree E, van Pel DM, Ushey K, Sipahimalani P, Bryan J, Rose AM, Hieter P,
PLoS Genet (2012) 8:e1002574-e1002574

LY2228820 dimesylate, a selective inhibitor of p38 mitogen-activated protein kinase, reduces angiogenic endothelial cord formation in vitro and in vivo.
Tate CM, Blosser W, Wyss L, Evans G, Xue Q, Pan Y, Stancato L,
J Biol Chem (2013) 288:6743-6753

Reducing inositol lipid hydrolysis, Ins(1,4,5)P3 receptor availability, or Ca2+ gradients lengthens the duration of the cell cycle in Xenopus laevis blastomeres.
Han JK, Fukami K, Nuccitelli R
J Cell Biol (1992) 116:147-156

Frequently Asked Questions (FAQs)

Can I use Hoechst™ dyes to do both DNA quantitation and cell number counting in plates?
Yes, this can be done in a 96-well plate-based format. Hoechst™ dyes will label all cells, live or dead (or even fixed).  If doing DNA quantitation, you would also have a row of known DNA concentrations to make a standard curve to compare against, up to 10 μg/mL.

I have a mix of live bacteria and platelet cells, and I need to be able to separate out the bacteria. Do you have a suggestion?
Platelet cells don't have a nucleus and thus lack nuclear DNA. Therefore, a cell-permeant, DNA-selective dye would label bacteria with greater intensity relative to minor staining of the platelet mitochondrial DNA. We recommend using a Hoechst nucleic acid stain.