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FITC Annexin V

Introduction

FITC Annexin V is Annexin V conjugated with our green fluorescent dye FITC. Annexin V is a calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, making itself available for Annexin V binding.

Our FITC Annexin V is provided as lyophilized power, which is enough for 100 assays.

Reconstitution:  We recommend a quick spin followed by reconstitution in 500 μl distilled water, and store at 4°C.

Applications

Cell apoptosis analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
A033 FITC Annexin V, 100 assays 490/519 25  μg $140

 


Protocol

Documents

Protocol (PDF): A033
MSDS (PDF):  MSDS-A033

Reference

Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
Logue SE, Elgendy M, Martin SJ,
Nat Protoc (2009) 4:1383-1395

Flow cytometry-based apoptosis detection.
Wlodkowic D, Skommer J, Darzynkiewicz Z,
Methods Mol Biol (2009) 559:19-32

Fluorescent biomembrane probe for ratiometric detection of apoptosis.
Shynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y,
J Am Chem Soc (2007) 129:2187-2193

Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay.
Pläsier B, Lloyd DR, Paul GC, Thomas CR, Al-Rubeai M
J Immunol Methods (1999) 229:81-95