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EasyProbes™ Propidium Iodide Dead Cell Stain

Introduction

EasyProbes™ Propidium iodide is a commonly used dead-cell stain that emits red fluorescence upon binding to DNA. Our EasyProbes™ Propidium Iodide Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population, and for counting dead cells, measuring apoptosis, and sorting cells based on DNA content.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Rapid staining of dead cells without wash steps

 

Suggestions for use
• In most cases 2 drops/1×106 cells and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• EasyProbes™ Propidium Iodide Dead Cell Stain is excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser when bound to DNA, with an emission maximum at 617 nm. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.

Specifications:  
Excitation/Emission: 535/617 nm
Shipping Condition: Ambient
Storage Conditions: 2~25ºC, protect from light
Cell Permeability: Cell-Impermeant
Product Size: 6 × 2.5 mL dropper bottles
   

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C059 EasyProbes™ PI Dead Cell Stain 535/617 6×2.5 mL $80

 
 
     

Documents

Protocol (PDF): C059   
MSDS (PDF):  MSDS-C059   
     

Reference:

Extension of Saccharomyces paradoxus chronological lifespan by retrotransposons in certain media conditions is associated with changes in reactive oxygen species.
VanHoute D, Maxwell PH,
Genetics (2014) 198:531-545

Modulation of medium pH by caulobacter crescentus facilitates recovery from uranium-induced growth arrest.
Park DM, Jiao Y,
Appl Environ Microbiol (2014) 80:5680-5688

Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy.
Zavodszky E, Seaman MN, Moreau K, Jimenez-Sanchez M, Breusegem SY, Harbour ME, Rubinsztein DC,
Nat Commun (2014) 5:3828-3828