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EasyProbes™ Hoechst 33342 Live Cell Stain

Introduction

EasyProbes™ Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence upon binding to the minor groove of dsDNA. Our EasyProbes™ Hoechst 33342 Live Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Hoechst 33342 is widely used for nuclear counterstaining, cell counting, apoptosis and cell cycle studies.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA

 

Suggestions for use
EasyProbes™ Hoechst 33342 Live Cell Stain may be added directly to cells in full media, or buffer solutions.
• In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained. In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• EasyProbes™ Hoechst 33342 Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter set.

Specifications:  
Excitation/Emission: 350/460 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Cell Permeability: Cell-Permeant
Product Size: 6 × 2.5 mL dropper bottles
   

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C057 EasyProbes™ Hoechst 33342 Live Cell Stain 350/460 6×2.5 mL $80

 
 
     

Documents

Protocol (PDF): C057   
MSDS (PDF):  MSDS-C057   
     

Reference:

A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
Medin CL, Valois S, Patkar CG, Rothman AL,
J Virol Methods (2015) 211:55-62

Centrifugo-magnetophoretic Purification of CD4+ Cells from Whole Blood toward Future HIV/AIDS Point-of-Care Applications.
Glynn M, Kirby D, Chung D, Kinahan DJ, Kijanka G, Ducrée J,
J Lab Autom (2013) –

Complex determinants in specific members of the mannose receptor family govern collagen endocytosis.
Jürgensen HJ, Johansson K, Madsen DH, Porse A, Melander MC, Sørensen KR, Nielsen C, Bugge TH, Behrendt N, Engelholm LH,
J Biol Chem (2014) 289:7935-7947

Frequently Asked Questions (FAQs)

I have a mix of live bacteria and platelet cells, and I need to be able to separate out the bacteria. Do you have a suggestion?
Platelet cells don't have a nucleus and thus lack nuclear DNA. Therefore, a cell-permeant, DNA-selective dye would label bacteria with greater intensity relative to minor staining of the platelet mitochondrial DNA. We recommend using a Hoechst nucleic acid stain.

I need a total cell stain to label cytoplasm and nuclei in live cells. What do you recommend?
You can use a combination of two dyes with overlapping blue emission. For cytoplasm, you can label the cell with CellView Blue CMAC. This can be combined with Hoechst 33342 for nuclei. Both dyes are imaged using a standard DAPI filter set.