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EasyProbes™ Green 488 Dead Cell Stain

Introduction

EasyProbes™ Green 488 Dead Cell Stain is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Our EasyProbes™ Green 488 Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. EasyProbes™ Green 488 Dead Cell Stain is a useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Rapid staining of damaged, dead, or fixed cells without wash steps

 

Suggestions for use
• EasyProbes™ Green 488 reagent may be added directly to cells in full media or buffer solutions.
• It easily stains nuclei of fixed cells and tissue slices

In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• EasyProbes™ Green 488 Dead Cell Stain is excited with a maximum at 500 nm when bound to DNA, with an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.

 

Specifications:  
Excitation/Emission: 500/530 nm
Shipping Condition: Ambient
Storage Conditions: 2~8ºC, protect from light
Cell Permeability: Cell-Impermeant
Product Size: 6 × 2.5 mL dropper bottles
 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C063 EasyProbes™ Green 488 Dead Cell Stain 500/530 6×2.5 mL $100

 
 
     

Documents

Protocol (PDF): C063   
MSDS (PDF):  MSDS-C063   
     

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