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EasyProbes™ DAPI Fixed Cell Stain

Introduction

EasyProbes™ DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence upon binding to the minor groove of dsDNA. Our EasyProbes™ DAPI Fixed Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. DAPI is widely used for nuclear and chromosome counterstain, showing a distinct banding pattern in chromosomes.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA

 

Suggestions for use
• In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• EasyProbes™ DAPI Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter set.

Specifications:  
Excitation/Emission: 358/460 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Cell Permeability: Cell-Impermeant
Product Size: 6 × 2.5 mL dropper bottles
   

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C058 EasyProbes™ DAPI Fixed Cell Stain 358/460 6×2.5 mL $80

 
 
     

Documents

Protocol (PDF): C058   
MSDS (PDF):  MSDS-C058   
     

Reference:

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