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EasyProbes™ ActinGreen 488 Stain

Introduction

EasyProbes™ ActinGreen 488 Stain is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Andy Fluor 488 dye. Our EasyProbes™ ActinGreen 488 Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops of stain solution to the cell slide to stain your cells. 

Features

  • High-affinity staining of F-actin with superior specificity compared to antibody methods
  • Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
  • Bright fluorescence signal

 

Suggestions for use

In most cases 2 drops of stain solution and an incubation of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• EasyProbes™ ActinGreen 488 Stain is excited with a maximum at 500 nm, with an emission maximum at 525 nm. It is detected through standard GFP and FITC filters.

Specifications:  
Excitation/Emission: 500/525 nm
Shipping Condition: Ambient
Storage Conditions: 2~8ºC, protect from light
Cell Permeability: Cell-Impermeant
Product Size: 2 × 2.5 mL dropper bottles
   

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C060 EasyProbes™ ActinGreen 488 Stain 500/525 2×2.5 mL $150

 
 
     

Documents

Protocol (PDF): C060   
MSDS (PDF):  MSDS-C060   
     

Reference:

 

Joshi B, Bastiani M, Strugnell SS, Boscher C, Parton RG, Nabi IR,
J Cell Biol (2012) 199:425-435

Drug-selected human lung cancer stem cells: cytokine network, tumorigenic and metastatic properties.
Levina V, Marrangoni AM, DeMarco R, Gorelik E, Lokshin AE,
PLoS One (2008) 3:e3077-e3077

Expression levels of a kinesin-13 microtubule depolymerase modulates the effectiveness of anti-microtubule agents.
Schimizzi GV, Currie JD, Rogers SL,
PLoS One (2010) 5:e11381-e11381

 

Publications

2017