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CFDA, SE

Introduction

CFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. CFDA, SE passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, this probe is well retained in cells.

Specifications:     
Excitation/Emission: 490/520 (after cleavage)
Shipping Condition: Ice pack
Storage Conditions: -20ºC, protect from light
Molecular Formula: C29H19NO11
Molecular Weight: 557.47
CAS Number: 150347-59-4  

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C032 CFDA, SE 490/520 25 mg $100 

Protocol
MSDS
COA

Documents

Protocol (PDF): C032
MSDS (PDF):  MSDS-C032
COA (PDF):  C032

Reference:

Nitric oxide-induced regulatory T cells inhibit Th17 but not Th1 cell differentiation and function.
Niedbala W, Besnard AG, Jiang HR, Alves-Filho JC, Fukada SY, Nascimento D, Mitani A, Pushparaj P, Alqahtani MH, Liew FY
J Immunol 2013; (191):1 164-170

Convergent differentiation: myeloid and lymphoid pathways to murine plasmacytoid dendritic cells.
Sathe P, Vremec D, Wu L, Corcoran L, Shortman K
Blood 2013; (121):1 11-19

Dual receptor T cells extend the immune repertoire for foreign antigens
He X, Janeway CA, Levine M, Robinson E, Preston-Hurlburt, P, Viret C, Bottomly K
Nat Immunol (2002) 3:127-127

Frequently Asked Questions (FAQs)

I’m trying to label my cells in suspension and track them over time by labeling with CFDA SE, but after labeling them they will no longer adhere to a surface (whereas unlabeled cells adhere well). I’ve tried to label at 10 and 20 μM. What is causing this?
This dye will bind to proteins via primary amines. Too high of a concentration can lead to cell toxicity or unintended modification of cell function. The solution is to reduce the concentration and/or staining time.