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Cell-Quant™ AlamarBlue Cell Viability Reagent

Introduction

Our Cell-Quant™ AlamarBlue Cell Viability Reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Features

  • Simple workflow: Add, incubate, and read
  • Flexible: Measure by colorimetric or fluorescent
  • High sensitivity: Detect as low as 100 cells
  • Safety: Non-toxic to cells
  • Low cost and reliable results

 

Specifications

Platform: Microplate Reader
Detection Method: Fluorescent, Colorimetric
Ex/Em: 530-560/590 nm
Product Size: 2500 assays

Applications

  • Cell proliferation analysis
  • Cell viability assays, including cytotoxicity
  • Cytokine assays

Figure 1. Quantitation of Jurkat cells using the Cell-Quant™ AlamarBlue Cell Viability Reagent. Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and incubated with AlamarBlue Cell Viability Reagent for 4 hr , fluorescence was measured at Ex/Em=545/590 nm.