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Caspase-3/7 Z-DEVD-R110 Assay Kit

Introduction

The Caspase-3/7 Z-DEVD-R110 Assay Kit provides a method for detecting apoptosis by providing a simple and reliable method for assaying caspase-3/7 activity. The kit can be used to continuously measure the activity of caspase-3/7 in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.
The rhodamine 110-derived substrate (Z-DEVD-R110) used in this assay is a non-fluorescent bisamide compound that, upon enzymatic cleavage, is converted in a two-step process to the fluorescent monoamide and then to the even more fluorescent R110 product. Both of these hydrolysis products exhibit spectral properties similar to those of fluorescein, with peak excitation and emission wavelengths of 496 nm and 520 nm, respectively.

 

In addition to the Z-DEVD–R110 substrate, the Caspase-3/7 Z-DEVD-R110 Assay Kit contains the reversible aldehyde inhibitor Ac-DEVD-CHO, as well as the reference standard R110. The Ac-DEVD-CHO inhibitor confirms that the fluorescence signals in both induced and control cell populations are due to the activity of caspase-3/7. The reference standard allows for quantification of the amount of R110 released in the reaction.

 

Specifications

Platform: Microplate Reader, Fluorometer
Detection Method: Fluorescent
Ex/Em: 496/520 nm
Product Size: 100 assays
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Ice pack

Applications

Cell apoptosis analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

To order

Buy Cat.No. Product Name Ex/Em Unit Price
A044 Caspase-3/7 Z-DEVD-R110 Assay Kit 496/520 100 assays $200

Protocol

 

 

Documents

Protocol (PDF): A044
MSDS (PDF):  MSDS-A044

References:

Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells.
Hug H, Los M, Hirt W, Debatin KM
Biochemistry (1999) 38:13906-13911

Fluorescent molecular probes V: a sensitive caspase-3 substrate for fluorometric assays.
Liu J, Bhalgat M, Zhang C, Diwu Z, Hoyland B, Klaubert DH
Bioorg Med Chem Lett (1999) 9:3231-3236

Härtel S, Zorn-Kruppa M, Tykhonova S, Alajuuma P, Engelke M, Diehl HA
Cytometry A (2003) 55:15-23