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Caspase-3/7 Substrate: (Z-DEVD)2-R110

Introduction

(Z-DEVD)2-R110 is a fluorogenic enzyme substrate of caspase-3/7 that is widely used for high-throughput assays. Peptides are covalently linked to each of the amino groups on rhodamine 110 (R110), which suppresses the fluorescence of the dye. When the peptides are cleaved by the caspase, the substrate is converted first to the fluorescent monoamide and then to R110, with a further increase in fluorescence. The substrate can be used to continuously measure enzyme activity in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader.

Specifications

Label: Rhodamine 110
Ex/Em: 500/520 nm
Detection Method: Fluorescent
Molecular Formula: C72H78N10O27
Molecular Weight: 1515.46
CAS Number:
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature
Applications
Caspase Substrate
 

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C284 (Z-DEVD)2-R110 500/520 5 mg $400

Documents

Datasheet (PDF): C284
MSDS (PDF): MSDS-C284

References:

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Photodynamic therapy and cell death pathways.
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Methods Mol Biol (2010) 635:35-46