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Apoptotic, Necrotic, and Healthy Cells Detection Kit

Introduction

Our Apoptotic, Necrotic, and Healthy Cells Detection Kit provides a convenient assay to detect apoptotic, necrotic, and total cells within the same cell population by flow cytometry or fluorescence microscopy. This kit contains three-color reagent:  Andy Fluor™ 488 annexin V used to stain apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface; propidium iodide (PI) used to stain necrotic cells with comprised membrane integrity; and Hoechst 33324 used to stain all cell nuclei. After staining a cell population, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and all cells show blue fluorescence.

 

 

Features:

  • Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay
  • Fast: A single 30 min staining step
  • Sensitive: Brighter fluorescence signal

Specifications

Platform: Fluorescence Cytometer, Fluorescence Microscopy
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm
Product Size: 50 assays
Storage Conditions: 4 ℃, protect from light
Shipping Condition: Ice pack

Applications

Cell apoptosis analysis.