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Annexin V Binding Assays

Introduction

Annexin V is a 35–36 kilodalton, Ca2+-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, phosphatidylserine is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, phosphatidylserine is translocated from the inner to the outer leaflet of the plasma membrane, exposing phosphatidylserine to the external cellular environment where it can be detected by annexin V conjugate (Figure 1).

Our fluorescent annexin V conjugates and kits provide quick and reliable detection methods for studying the externalization of phosphatidylserine in apoptotic cells. These conjugates and kits are very useful for flow cytometry or fluorescence microscopy to assess mixed populations of apoptotic and nonapoptotic cells.

Features:

  • Sensitivity—Andy Fluor™ dye conjugates for brighter signals
  • Flexibility—Conjugates available for different lasers and detection channels
  • Simplicity—Assay procedure typically completed in less than 30 minutes

 

                           Figure 1. PS translocation in apoptotic cell.

Table 1. Selection Guide for Annexin V Binding Assay Kit

Cat. No. Product Name Probe for Apoptotic Cells (Ex/Em, nm) Probe for Necrotic Cells (Ex/Em, nm)  Unit
A025 eGFP Annexin V and PI Apoptosis Kit eGFP Annexin V (475/509) PI (535/617) 100 assays
A026 FITC Annexin V and PI Apoptosis Kit FITC Annexin V (490/519) PI (535/617) 100 assays
A027 Andy Fluor™ 488 Annexin V and PI    Apoptosis Kit Andy Fluor™ 488 Annexin V (495/520) PI (535/617) 100 assays
A028 Apoptotic, Necrotic, and Healthy Cells Detection Kit Andy Fluor™ 488 Annexin V (495/520) PI (535/617) 50 assays

Table 2. Selection Guide for Annexin V Reagent

Cat. No. Product Name Laser Emission Recommended filter Unit
A029A A029B Annexin V, recombinant 0.5 mg
1.0 mg
A030 eGFP Annexin V 488 nm 509 nm 530/30 nm 100 assays
A031 Biotin-X Annexin V 100 assays
A032 Andy Fluor™ 350 Annexin V UV 445 nm 450/40 nm 100 assays
A033 FITC Annexin V 488 nm 519 nm 530/30 nm 100 assays
A034 Andy Fluor™ 488 Annexin V 488 nm 520 nm 530/30 nm 100 assays
A035 Andy Fluor™ 555 Annexin V 532 nm 565 nm 575/26 nm 100 assays
A036 Andy Fluor™ 568 Annexin V 561 nm 603 nm 610/20 nm 100 assays
A037 Andy Fluor™ 594 Annexin V 590 nm 617 nm 630/20 nm 100 assays
A038 Andy Fluor™ 647 Annexin V 633 nm 665 nm 661/8 nm 100 assays
A039 Andy Fluor™ 680 Annexin V 635 nm 700 nm 700/20 nm 100 assays