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Andy Fluor™ 488 Annexin V and PI Apoptosis Kit

Introduction

Our Andy Fluor™ 488 Annexin V and PI Apoptosis Kit is used to detect the externalization of phosphatidylserine in apoptotic cells. This kit provides a sensitive two-color assay that employs our green-fluorescent Andy Fluor™ 488 annexin V and a ready-to-use solution of the red-fluorescent propidium iodide nucleic acid stain.  Propidium iodide is impermeant to live cells and apoptotic cells but stains necrotic cells with red fluorescence, binding tightly to the nucleic acids  in the cell. After staining a cell population with Andy Fluor™ 488 annexin V and propidium iodide in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished using a flow cytometer with the 488 nm spectral line of an argon-ion laser for excitation.

 

Specifications

Platform: Fluorescence Cytometer
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm
Product Size: 100 assays
Storage Conditions: 4 ℃, protect from light
Shipping Condition: Ice pack

Applications

Cell apoptosis analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 1. Jurkat cells treated with 10 μM camptothecin for four hours (right panel) or untreated (as control, left panel). Cells were then treated with the reagents in the kit, followed by flow cytometric analysis.

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A027 Andy Fluor™ 488 Annexin V and PI Apoptosis Kit 495/520 100 assays $180

Protocol

Documents

Protocol (PDF): A027
MSDS (PDF):  MSDS-A027

Reference

Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
Logue SE, Elgendy M, Martin SJ,
Nat Protoc (2009) 4:1383-1395

Flow cytometry-based apoptosis detection.
Wlodkowic D, Skommer J, Darzynkiewicz Z,
Methods Mol Biol (2009) 559:19-32

Fluorescent biomembrane probe for ratiometric detection of apoptosis.
Shynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y,
J Am Chem Soc (2007) 129:2187-2193

Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay.
Pläsier B, Lloyd DR, Paul GC, Thomas CR, Al-Rubeai M
J Immunol Methods (1999) 229:81-95