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Andy Fluor™ 350 Annexin V

Introduction

Andy Fluor™ 350 Annexin V is Annexin V conjugated with our blue fluorescent dye Andy Fluor™ 350. Annexin V is a calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, making itself available for Annexin V binding.

Andy Fluor™ 350 is a superior alternative to the traditional blue fluorescent dye AMCA owing to its exceptional brightness and photostability. Andy Fluor™ 350 has brightness and photostability comparable to Alexa Fluor® 350.

Our Andy Fluor™ 350 Annexin V is provided as lyophilized power, which is enough for 100 assays.

Reconstitution:  We recommend a quick spin followed by reconstitution in 500 μl distilled water, and store at 4°C.

Applications

Cell apoptosis analysis.