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ActinBlue™ 350 stain

Introduction

ActinBlue™ 350 stain is used for F-actin stain. The blue fluorescent probe binds to F-actin with nanomolar affinity and it is highly photostable. Fluorescently labeled phallotoxins are very useful tool for investigating the distribution of F-actin. Labeled phallotoxins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants and animals. ActinBlue™ 350 stain is provided as 40 unit size (C051T) and 300 unit size (C051).

Specifications:     
Excitation/Emission: 347/440 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Cell Permeability: Cell-impermeant
Unit Size: 40 unit, 300 unit
Color: Blue

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price

C051T
C051
ActinBlue™ 350 stain 347/440 40 unit
300 unit
$80
$200

Protocol
MSDS
COA

Documents

Protocol (PDF): C051
MSDS (PDF):  MSDS-C051 
COA (PDF):  COA-C051 

Reference:

ADP ribosylation factor 6 is activated and controls membrane delivery during phagocytosis in macrophages.
Niedergang F, Colucci-Guyon E, Dubois T, Raposo G, Chavrier P
J Cell Biol (2003) 161:1143-1143

A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii.
Carey KL, Westwood NJ, Mitchison TJ, Ward GE
Proc Natl Acad Sci U S A (2004) 101:7433-7438

Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.
Goffin JM, Pittet P, Csucs G, Lussi JW, Meister JJ, Hinz B
J Cell Biol (2006) 172:259-268