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Acridine orange

Introduction

Acridine orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine orange has also been used as a lysosomal dye. Acridine orange is supplied as 10 mg/mL solution in water.

Specifications:  
Excitation/Emission: 500/526 nm (DNA);  460/650 nm (RNA)
Shipping Condition: Ambient
Storage Conditions: 2-8ºC, protect from light
Molecular Formula: C17H20ClN3
Molecular Weight: 301.82
CAS Number: 65-61-2  

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C014 Acridine orange  500/526 nm (DNA); 460/650 nm (RNA) 1 ml $25 

Protocol
MSDS
COA

Documents

Protocol (PDF): C014
MSDS (PDF):  MSDS-C014
COA (PDF): COA-C014

Reference:

Computer-Assisted Laser Scanning and Video Microscopy for Analysis of Cryptosporidium parvum Oocysts in Soil, Sediment, and Feces.
Anguish LJ, Ghiorse WC
Appl Environ Microbiol (1997) 63:724-724

Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments.
Fuller ME, Streger SH, Rothmel RK, Mailloux BJ, Hall JA, Onstott TC, Fredrickson JK, Balkwill DL, DeFlaun MF
Appl Environ Microbiol (2000) 66:4486-4496

The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station.
Leys N, Baatout S, Rosier C, Dams A, s’Heeren C, Wattiez R, Mergeay M,
Antonie Van Leeuwenhoek (2009) 96:227-245

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