You are here: Home > FAQs >

Product FAQs

Fluorescent labeling and detection

Nucleic Acid Quantitation

Answer: The dye in the iQuant™ dsDNA Qantitation kits associates with dsDNA, ssDNA or RNA,causing nucleic acid fluorescence.
Answer: The fluorescence intensity is proportional to the amount of dsDNA, ssDNA or RNA. The dyes in different kits are different, and have different functions in nucleic acid quantitation.
Answer: Our iQuant™ ssDNA Quantitation Kit is suitable for quantitating ssDNA or oligonucleotides (18-mer or longer), and will not detect dNTPs. The sensitivity ranges from 2-200 ng of ssDNA.
 

Nucleic acid gel stains

Answer: One year.
Answer: Yes, although we recommend using it only 1 time. Multiple uses can decrease the signal sensitivity.
 
Answer: Yes. However, we recommend storing gels pre-made with GreenView at 4 degrees in the dark
 

Protein gel stains

Answer: eLuminol™ has two excitation wavelengths: 300 nm and 460 nm. The emission wavelength is around 600 nm.
 

Click chemistry tools

Answer: You should be able to, but we have only tested it on mouse tissues. You will need to optimize the protocol for staining zebrafish embryos.
 
Answer: The assay involves two steps. First, incorporate EU into RNA. Then, detect EU by click chemistry.The efficiency of incorporating EU into RNA is highly variable depending on the cell lines. It requires the customer to optimize the incubation conditions.Generally, once EU is incorporated into RNA, it can be efficiently detected by Click chemistry.We recommend you do side by side comparison with a known control cell line to confirm if incorporating EU into RNA is the problem in your specific cell line. Also you can try EdU instead of EU.
Answer: Yes.
Answer: Yes.
 

Secondary antibodies and streptavidin

Answer: The Coomassie stain will quench the fluorescence from the Phos-Tag stain. So, the Coomassie stain needs be used after Phos-Tag stain.
Answer: Our iLink™ Antibody Labeling Kits are optimized for labeling 50-100 µg of antibody per reaction (each vial).
 
Answer: Yes.
Answer: Yes. If the sample contains a lot of Tris buffer, add PBS buffer and centrifuge, and repeat 2-3 times, which will get rid of most of the Tris buffer.
 
Answer: Our iLink™ Antibody Labeling Kits cannot be used for the labeling of serum based antibodies.
 

Probes for reactive oxygen species

Answer: Yes.
Answer: It is a single isomer.

Cell structure probes

Answer: Yes.
Answer: Yes. The reactive dyes, such as CFDA, SE (Cat# C032), will remain in the cytoplasm.
 

Andy Fluor™ and Cy® Dyes

Answer: The Andy Fluor conjugates have equivalent quality and performance at a fraction of the price.
 

Cell proliferation and viability

Answer: Ideally, yes, you need to test each cell lines for optimal incubation time. For tumor cell lines, overnight incubation will be good.
 
Answer: No, the fluorescent signal is in the media.
 
Answer: You can store in a refrigerator for later use.
 

Apoptosis

Answer: Yes. We recommend that you first optimize for the tissue type that you are working with.
Answer: Our apoptosis kits (A025, A026, A027, A028) contain the component PI, which could be used for cell cycle and cell viability assays.
 
Answer: It doesn’t matter how you culture the cells. You will harvest the cells at a particular cell density, then add reagents, as explained in the protocol.
 
Answer: Unfortunately, we have not made this comparison.
 
Answer: This kit can be only used in cell culture. For tissue sections, please choose our TUNEL assay kits.
 
Answer: Andy Fluor™ 594 or 647 Annexin V both are good choices for red fluorescent detection, just make sure your detection instrument is equipped with the corresponding Ex/Em filters.