OmicsLink™ Validated Human Kinome shRNA Clones and Genome-Wide shRNA Clones for Human and Mouse Genes |
Available Now: Validated Human Kinome shRNA Clones
- shRNA clones are immediately available against 350 human kinome members.
- All are 19mer shRNAs designed for high KD effect and low off-target effect.
- The shRNA target sequences were cloned into a mammalian expression vector (psi-H1). Super H1 is used to drive shRNA expression. psi-sH1 vector also contains an EGFP gene driven by CMV promoter that can be used to measure transfection efficiency.
- The KD effect of these shRNA clones in this collection have been validated through a reporter functional assay system.
- 350 human kinome shRNA clones
- Corresponding human kinome ORF clones
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Genome-Wide Human and Mouse shRNA Clones in Lentiviral and Other Vector Types
- Varying lengths (19 to 29 bases), optimized for different genes
- Lentiviral and CMV based vectors
- Stable and transient gene silencing
- Efficient transduction into non-dividing and difficult to transfect target cells
- Fully sequenced
- Validated human kinome shRNA clones
- Wild type or mutant rescue ORF clones for silenced genes
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shRNA Clones Available in Multiple Vector Systems
Vector |
Promoter |
Reporter Gene |
Selecting Marker |
psiLv-H1 |
Lenti-H1 |
CMV-EGFP |
Puromycin |
psiLv-U6 |
Lenti-U6 |
CMV-EGFP |
Puromycin |
psi-H1 |
H1 |
CMV-EGFP |
Puromycin |
psi-U6 |
U6 |
CMV-EGFP |
Puromycin |
ORF Expression Clones are also available in more than 80 other expression vector systems with different promoters and various N- or C-terminal tags: His, GST, MBP, GFP, YFP, AviTag™, HA, Flag, SUMO, and more. |
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