*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Promotion ends June 30, 2025. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
Product Information
GeneCopoeia’s ExoCt™ RT-qPCR System allows you to analyze gene expression by real-time qPCR directly from exosomes without any RNA purification. The ExoCt™ First-Strand cDNA Synthesis Kit contains ExoCt™ RT-for-All™ Buffer and ExoCt™ PAP/RTase Mix, which were specifically developed for robust first-strand cDNA synthesis covering a variety of RNA templates, including miRNA, mRNA, LncRNA, etc., from exosome lysates in a single reaction. The ExoCt™ SYBR® Green RT-qPCR Kit includes both RT and qPCR reagents, which combines PCR technology and SYBR® Green to make fast and accurate quantification of exosome RNAs. The system includes the ExoCt™ First-Strand cDNA Synthesis Kit and the ExoCt™ SYBR® Green RT-qPCR Kit.
Advantages
- Direct detection of gene expression from exosomes without any RNA purification
- Convenient reverse transcription of all exosomal miRNA, mRNA, and LncRNA in one reaction simultaneously
- Flexible analysis and quantitation of different kinds of RNA in downstream qPCR reactions
Simple Workflow
Isolated exosomes are lysed by adding ExoCt™ Lysis Buffer, then heating at 37°C for 10 min and 75°C for 10 min. Exosome lysates are used directly for ExoCt™ RT reactions, and the cDNA products can be used for qPCR to detect miRNA, mRNA, and LncRNA, etc.
Performance Data
Figure 1. Comparing RT-qPCR results from exosome lysates and exosome-extracted RNA. 3 x 107 cells were cultured in 175mm plate until reaching 70% to 90% confluency. 40ml cells culture media were harvested and exosomes were isolated using ExoSure™ Exosome Isolation Kit. Exosomes from HEK393(red), MSC(grey), H2228(yellow), H3122(blue) were either lysed with GeneCopoeia’s ExoCt™ Lysis Buffer, or the RNA was extracted from them. Exosome lysates, or extracted RNA were reverse-trascribed to cDNA using ExoCt™ RT regent. Then the cDNA was used for gene expression detection.
Figure 2. Reverse transcribing miRNA, mRNA, and LncRNA simutaneouly in one tube using RT-For-All™ RT regent. 3 x 107 cells were cultured in 175mm plate until reaching 70% to 90% confluency. 40ml cells culture media were harvested and exosomes were isolated using ExoSure™ Exosome Isolation Kit. Exosomes were lysed with GeneCopoeia’s ExoCt™ Lysis Buffer. Exosome lysates were reverse transcribed to cDNA with ExoCt™ RT-For-All™ regent. The cDNA was used for gene expression detection. A. Exosome lysates from HEK293 cells. B. Exosome lysates from H3122 cells.
Figure 3. RT-qPCR detection using exosome lysates. 3 x 107 cells were cultured in 175mm plate until reaching 70% to 90% confluency. 40ml cells culture media were harvested and exosomes were isolated using ExoSure™ Exosome Isolation Kit. Exosomes were lysed with GeneCopoeia’s ExoCt™ Lysis Buffer. Exosome lysates were either reverse transcribed into cDNA with ExoCt™ RT-For-All™ regent or Takara RT regent. The cDNA was then used for gene expression detection. A. Exosome lysates from MSC cells. B. Exosome lysates from H3122 cells.
