AAV Serotype Testing Kit | GeneCopoeia™

Product Introduction

AAVPrime™ AAV Serotype Testing Kit

GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (Cat. No. AA320) contains 9 premade GFP-expressing AAV in serotypes 1, 2, 3, 6, 7, 8, 9, DJ and DJ/8.

AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit Plus (Cat. No. AA321) contains 12 premade GFP-expressing AAV in serotypes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, DJ and DJ/8. All AAV particles are offered at 5 ×10¹² GC/ml, in 25 µL stocks, except serotypes 4 and 10 are offered at 5 ×10¹¹~10¹² GC/ml. These kits are ideal for testing the infection efficiency of AAV serotypes in different cell types.

Figure 1. AAV of different serotypes carrying GFP were used to transduce the H1299 cell line as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.

AAVPrime™ AAV-qPCR Titration Kits

The AAV-qPCR Titration Kits are designed for quick and simple titration of Adeno-associated virus (AAV) particles using real-time quantitative PCR (qPCR), it can determines titration of all AAV serotypes as long as the ITR domains of the AAV genome is of AAV2 origin. The number of copies of the AAV genome is quantified using TaqMan –based qPCR.

The kits include the DNase I (RNase-free), AAV Lysis buffer, AAV-TaqMan qPCR mix (2x), AAV-Probe, and standard control.

AAV-293Ta packaging cell line

GCI-AAV-293Ta packaging cells for AAV production using optimized packaging plasmids with AAV vector-based constructs.

Order information

Cat #	Product Description	Titer	Size	Price
^NEW!AA321	AAVPrime™ AAV Serotype Testing Kit Plus	5 × 10¹¹ – 5 x 10¹² GC/mL	12 × 25 µL	$1509
AA320	AAVPrime™ AAV Serotype Testing Kit	5 × 10¹² GC/mL	9 × 25 µL	$860
^NEW!AA301	AAVPrime™ AAV-qPCR Titration Kit	–	20 DNase reactions, 50 qPCR reactions	$250
^NEW!AA302	AAVPrime™ AAV-qPCR Titration Kit	–	40 DNase reactions, 100 qPCR reactions	$370
^NEW!AA316	AAVPrime™ 293Ta Cell Line	–	1.5 x 10^6 cells	$270

^NEW!AAV of different serotypes expressing GFP can be ordered separately as AAV control particles.

AAV of different serotypes expressing GFP

Cat #	Product Description	Titer	Size	Price
AA601-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 1. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA601-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 1. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA001-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 2. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA001-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 2. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA801-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 3. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA801-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 3. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA074-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 4. Promoter: CMV.	≥ 5×10¹¹ GC/mL	25 µL	$379
AA074-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 4. Promoter: CMV.	≥ 5×10¹¹ GC/mL	100 µL	$541
AA084-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 5. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA084-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 5. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA097-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 6. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA097-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 6. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA107-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 7. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA107-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 7. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA117-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 8. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA117-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 8. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA016-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 9. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA016-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 9. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541
AA127-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 10. Promoter: CMV.	≥ 5×10¹¹GC/mL	25 µL	$379
AA127-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: 10. Promoter: CMV.	≥ 5×10¹¹GC/mL	100 µL	$541
AA201-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: DJ. Promoter: CMV.L	≥ 5×10¹² GC/mL	25 µL	$379
AA201-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: DJ. Promoter: CMV.L	≥ 5×10¹² GC/mL	100 µL	$541
AA401-025	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: DJ/8. Promoter: CMV.	≥ 5×10¹² GC/mL	25 µL	$379
AA401-100	eGFP AAVPrime™ Purified Adeno-associated Viral Particles Serotype: DJ/8. Promoter: CMV.	≥ 5×10¹² GC/mL	100 µL	$541

Related products

Resources

Product Datasheet: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit Plus
User Manual: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit Plus
Technical Note: Choosing Between Lentivirus and Adeno-associated Virus For DNA Delivery

FAQs

Why do different AAV serotypes show tissue tropism?

Answer: All AAV serotypes are non-enveloped, single-stranded DNA parvoviruses. They share some properties including genome size, organization, and similar inverted terminal repeats. Despite similarities, different serotypes display significant differences in transduction efficiency and tissue tropism. AAV cellular entry is determined by its interactions with specific cell surface glycans and proteinaceous receptor(s). Different AAV serotypes have evolved distinctive interactions with the same receptor, therefore displaying various tissue tropism.

Which serotype should I use in my experiment?

Answer:

Serotype	Primary Target Tissues	Description
AAV-1	Muscle	Best for cardiac muscle, skeletal muscle, neuronal and glial tissue.
AAV-2	Muscle, Liver, Retina	Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain.
AAV-3	Megakaryocytes	Best for megakaryocytes, muscle, liver, lung, and retina.
AAV-4	Retina	Best for neurons, muscle, brain, and retina.
AAV-5	Lung	Best for lung, neurons, synovial joint, retina, and pancreas.
AAV-6	Muscle, Lung	Best for lung, liver, and heart.
AAV-7	Muscle, Retina, Neurons	Best for muscle, neurons, and liver.
AAV-8	Liver	Best for muscle, brain, liver, and retina.
AAV-9	Various	Best for muscle, heart, liver, lung, and brain.
AAV-10	Pleura, CNS	Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver.
AAV-DJ	Various	A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro.
AAV-DJ/8	Various	A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo.

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Price:	$1,509.00 $860.00 $250.00 $370.00 $270.00
Catalog#:	AA321 AA320 AA301 AA302 AA316
XX:	12 x 25 µl (5 x 10¹² GC/ml) 9 x 25 µl (5 x 10¹² GC/ml) 20 DNase reactions, 50 qPCR reactions 40 DNase reactions, 100 qPCR reactions 1.5 x 10^6 cells
Size:	12 x 25 µl (5 x 10¹² GC/ml) 9 x 25 µl (5 x 10¹² GC/ml) 20 DNase reactions, 50 qPCR reactions 40 DNase reactions, 100 qPCR reactions 1.5 x 10^6 cells
Qty:
Manual:	Download Download Download Download Download

AAVPrime™ Adeno-associated Virus Serotype Testing Kit

AAVPrime™ AAV Serotype Testing Kit Plus (12 eGFP-expressing AAV) AAVPrime™ AAV Serotype Testing Kit (9 eGFP-expressing AAV) AAVPrime™ AAV-qPCR Titration Kits AAVPrime™ AAV-qPCR Titration Kits AAVPrime™ 293Ta Cell Line

AAVPrime™ AAV Serotype Testing Kit

AAVPrime™ AAV-qPCR Titration Kits

AAV-293Ta packaging cell line

^NEW!AAV of different serotypes expressing GFP can be ordered separately as AAV control particles.

Product Datasheet: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit Plus
User Manual: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit Plus
Technical Note: Choosing Between Lentivirus and Adeno-associated Virus For DNA Delivery

AAVPrime™ Adeno-associated Virus Serotype Testing Kit

AAVPrime™ AAV Serotype Testing Kit Plus (12 eGFP-expressing AAV) AAVPrime™ AAV Serotype Testing Kit (9 eGFP-expressing AAV) AAVPrime™ AAV-qPCR Titration Kits AAVPrime™ AAV-qPCR Titration Kits AAVPrime™ 293Ta Cell Line

AAVPrime™ AAV Serotype Testing Kit

AAVPrime™ AAV-qPCR Titration Kits

AAV-293Ta packaging cell line

NEW! AAV of different serotypes expressing GFP can be ordered separately as AAV control particles.

Product Datasheet: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit PlusUser Manual: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit PlusTechnical Note: Choosing Between Lentivirus and Adeno-associated Virus For DNA Delivery

^NEW!AAV of different serotypes expressing GFP can be ordered separately as AAV control particles.

Product Datasheet: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit Plus
User Manual: AAVPrime™ Serotype Testing Kit / AAVPrime™ Serotype Testing Kit Plus
Technical Note: Choosing Between Lentivirus and Adeno-associated Virus For DNA Delivery