For high throughput studying protein-protein interaction
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More than 40,000 individual full length ORFs of human and mouse genes are available in OmicsLink™ AD-ORF fusion and BD-ORF fusion expression vectors, enabling high-throughput studies of protein-protein interactions in vivo.
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- For two-hybrid analyses, the gene encoding "bait" protein is cloned into OmicsLink™ pBD vector resulting in a fusion protein with the GAL4 DNA binding domain. The gene encoding the "target" protein is cloned into OmicsLink™ pAD, resulting in fusion with Gal4 transcription activation domain.
- The two plasmids are co-transformed into yeast cells. If the "bait" and "target" proteins interact, the Gal4 binding and activation domains are brought together, which in turn activates the lacZ reporter gene. Therefore, the ability of the "bait" and "target" to interact can be determined based on the phenotypes of the transformed yeast cells.
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ORF Expression Clones are also available in 100+ expression vector systems with different promoters and various N- or C-terminal tags: His, GST, MBP, eGFP, eYFP, AviTag™, HA, Flag, SUMO, and more.
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