| OmicsLink™ ORF Expression Clones (Full Length, Without 5' and 3' UTRs) |
OmicsLink™ ORF expression clone collections offer multiple sets of validated and high quality full length protein coding ORF cDNA clones (from start to stop codons) for more than 20,000 human and 15,000 mouse genes in various expression vector systems in ready-for-expression formats. OmicsLink™ clones have a wide range of applications in the areas of genomics, proteomics and other fields of biomedical and pharmaceutical research and development.
|
 |
| | Multiple Sets of OmicsLink™ ORF Expression Clones Available and Ready for Expression in Bacterial, Mammalian, Yeast and Insect Cells |
|
|
| OmicsLink™ Expression ORF Clones are Available in the Following Vector Systems |
| Vector |
Promoter |
Host Cell |
Selecting
Marker |
Tag |
Protease
Site |
| pDONR™ Vector |
ORFEXPRESS™ Shuttle Clone
(Gateway® Entry Clone) |
N/A |
N/A |
N/A |
N/A |
|
| Vector |
Promoter |
Host Cell |
Selecting
Marker |
Tag |
Protease
Site |
| pReceiver-I01 |
AcMNPV polyhedrin |
Insect cell |
N/A |
N-His |
Tev |
|
|
|
Ready for in vitro and in vivo Expression |
| Expression of proteins of your interest is now as easy as Click and Express by click-select-order of OmicsLink™ clones and expressing them immediately in your laboratory. OmicsLink™ now offers clones in several expression vectors with a variety of promoters, optimal translation signals (for example: Shine-Dalgarno and Kozak for E.coli and mammalian cell expression systems, respectively), and features, which makes these clones suitable for expression and functional studies in various cell systems. GeneCopoeia also offers OmicsMate™ service to construct expression clones for other vector systems that are desired by customers but not yet included in our offered product line. |
|
Application of OmicsLink™ Clones in Systems and Integrated Biology |
- Express proteins in E.coli cells
- Express proteins in mammalian cells by reverse transfection
- Produce proteins in in vitro cell-free translation systems
- Express proteins with unique tag that facilitates purification and down-stream analysis
- Generate probes of in situ hybridization for tissue or organ gene expression profiling
- Produce templates for esiRNA for gene silencing functional analysis
- Validation of shRNA/miRNA function
|
Comparison with Whole Transcript cDNA Clones:
| Clone Characteristics |
OmicsLink™ ORF clones |
Whole Transcript cDNA Clones |
| Gene transcript | ORF
(Start to stop only) | Whole transcripts including 5' and 3' UTRs |
| Expression ready | Yes | Some are, most will need further subcloning procedures to remove 5' and 3' UTRs |
Mammalian cell transfection
- Transicent Transfection
- Stable Transfection |
Yes
Yes |
Only a small number
No |
Unique protein tags
- Protein-Molecule interactions
- Concentration of lowly expressed proteins
- Affinity purification of expressed proteins |
Yes
Yes
Yes
Yes
|
No
No
No
No
|
| shRNAi validation and rescue |
Yes |
No |
| miRNA and miRNA target validation and rescue |
Yes |
No |
| Produce proteins via in vitro cell-free translation systems |
Yes |
No |
| production Template for esiRNA |
Yes |
No |
| Probe generations for in situ hybridization |
Yes |
No |
|
| Quality Assurance |
| OmicsLink™ clones have been constructed from collections of carefully curated and verified full length cDNA plasmids and high quality cDNA libraries. The high quality of these clones is ensured by a stringent multiple-step QC/QA process strictly followed before every single clone is shipped to customers. |
