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| EnzyStart™ (US Patent Issued) |
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| EnzyStart™ has been developed to increase the specificity and sensitivity of DNA amplification and can be used for all thermosable DNA polymerases in PCR, RT-PCR, Q-PCR and other PCR related techniques. The principle of EnzyStart™ is based upon a combination of the use of a pair chemically modified oligonucleotide primers with a blocker at their 3' end and a 3'TBGRE (3' Terminal Blocker Group Remove Enzyme, a thermostable protein). In modified primer, there is not a hydroxyl group at 3'end, even though the primer is annealed to non-specific site it can not be extended by any DNA polymerase. The 3'TBGRE has no detectable activity at low temperature but has maximal activity to remove the 3' end blocker at temperatures between 55 oC to 80 oC in the complete amplification reaction mixture. The conversion of the 3' end modified primers to conventional primers by the 3'TBGRE at temperatures above 55 oC supplies the amplification reaction with functional primer that bind to the desired template sequence and are extended by DNA polymerase in next cycle. It avoids the extension of mis-annealed primers at the non-specific sites of target DNA and/or RNA at ambient temperature that commonly occurs with conventional method. |
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