GLuc-ON Transcriptional Response Assays

Introduction

GeneCopoeia's GLuc-ON™ transcriptional response element (TRE) clones enable rapid and sensitive signal pathway analysis in mammalian cell lines. Using a secreted Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ TRE  clones are designed for robust and sensitive activation of specific signaling pathways in response to enviromental stimuli or other experimental manipulation. Each GLuc-ON™ TRE clone comes either in transfection-ready or lentiviral format, and contains tandem repeats of a TRE placed upstream of a minimal CMV promoter and the Gluc gene. 

GeneCopoeia is also now offering cell lines stably expressing Transcriptional Response Elements. Click on the TRE stable cell lines tab to order, or visit the Transcriptional Response Element Stable Cell Lines page to learn more.

Advantages

  • Live cell assays. Naturally secreted GLuc reporter makes lysing of cells unnecessary.
  • Real-time study. Data is generated quickly, closely resembling real-time activities
  • High-throughput compatible.
 

 

Applications

GLuc-ON™ TRE clones offer powerful tools for several applications, including:

  • Drug discovery or validation. Identify small molecules that either stimulate or inhibit a signaling pathway of interest.
  • Analyze response of cells to proteins and peptides.
  • Analyze response of cells to hormones.
  • Functional genomics. Analyze the biological effects on a signaling pathway in response to gene activation, overexpression, knockdown, knockout, or mutagenesis (Figure 1).
  • Viral infection. Analyze response of cells to viruses.

 

Figure 1. Application example: Functional genomics using TRE clones. HEK293a cells were co-transfected with Oct4 TRE clone + GFP expression clone, negative control clone + Oct4 expression clone, or Oct4 TRE clone + Oct4 expression clone. Transfected cells were incubated for 48 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation. 

 

How do GLuc-ON™ TRE clones work?

GLuc-ON™ TRE response elements are engineered to provide strong activation of GLuc expression with low background. The GLuc reporter is a secreted protein, permitting convenient and rapid detection in the cell culture medium without lysing the cells (Figure 2).

 

 

Figure 2. How GLuc-ON TRE clones work.

TRE non-viral clones

GLuc-ON™ Transcriptional Response Element (TRE) Clones

Buy Cat.No. Product name Price
TR100 CREB TRE clone for cAMP/PKA signaling pathway $350
TR101 NFAT TRE clone for PKC/Ca++ signaling pathway $350
TR102 TCF/LEF TRE clone for Wnt signaling pathway $350
TR103 Oct4 TRE clone for Oct4 signaling pathway $350
TR104 HNF4 TRE clone for HNF4 signaling pathway $350
TR105 JNK TRE clone for SAPK/JNK signaling pathway $350
TR106 PKC TRE clone for PKC/MAPK signaling pathway $350
TR107 EGR1 TRE clone for EGR1 pathway $350
TR109 MEF2 TRE clone for MEF2 signaling pathway $350
TR110 ERSE TRE clone for ER stress pathway $350
TR111 GR TRE clone for glucocorticoid receptor signaling pathway $350
TR112 PPAR TRE clone for peroxisome proliferator-activated receptor signaling pathway $350
TR113 PR TRE clone for progesterone receptor signaling pathway $350
TR114 LXRa TRE clone for liver X receptor signaling pathway $350
TR115 NFκB TRE clone for NFκB-regulated signaling pathway $350
SEAP-PA01 Secreted alkaline phosphatase (SEAP) clone for normalization $395
TR001 TRE negative control clone $300
GAPDH-PG02 GAPDH positive control clone $375

 

 

EndoFectin transfection reagents

Buy Cat.No. Product name Price Data sheets
EFM1004-01 EndoFectin™ Max transfection reagent (1 mL) $295 Download
EFM1004-02 EndoFectin™ Max transfection reagent (3 mL) $589
EFL1001-01 EndoFectin™ Lenti transfection reagent (1 mL) $245 Download
EFL1001-02 EndoFectin™ Lenti transfection reagent (3 mL) $489
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $355 Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $705
EF007 EndoFectin™ Expi293 transfection reagent (1 mL) $320  
EF008 EndoFectin™ Expi293 transfection reagent (3 mL) $640  

 

TRE lentiviral clones

GLuc-ON™ Transcriptional Response Element (TRE) Lentiviral Clones

Buy Cat.No. Product name Price
TR200 CREB TRE lentiviral clone for cAMP/PKA signaling pathway $450
TR201 NFAT TRE lentiviral clone for PKC/Ca++ signaling pathway $450
TR202 TCF/LEF TRE lentiviral clone for Wnt signaling pathway $450
TR206 PKC TRE lentiviral clone for PKC/MAPK signaling pathway $450
TR207 EGR1 lentiviral TRE clone for EGR1 pathway $450
TR002 TRE negative control lentiviral clone $400

 

 

Lenti-Pac™ Lentiviral Packaging Kits

 
Buy Catalog# Product Description Manual or datasheets Price
LT001 Lenti-Pac HIV Expression Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent /wp-content/uploads/2017/06/Lenti-Pac-HIV-Expression-Packaging-Kit-Manual.pdf inquire
LT002 Lenti-Pac HIV Expression Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent HIV packaging kit manual inquire
LT005 Lenti-Pac HIV qRT-PCR Titration Kit (20 RT reactions, 50 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. http://www.genecopoeia.com/wp-content/uploads/2017/06/Lenti-Pac_qRT-PCR_Titration_Kit_manual.pdf inquire
LT006 Lenti-Pac HIV qRT-PCR Titration Kit (40 RT reactions, 100 PCR reactions) qRT-PCR based lentiviral titration to determine the copy numbers of HIV lentiviral particles. http://www.genecopoeia.com/wp-content/uploads/2017/06/Lenti-Pac_qRT-PCR_Titration_Kit_manual.pdf inquire
LT007 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles http://www.genecopoeia.com/wp-content/uploads/2017/06/Lenti-Pac_Lentivirus_Concentration_Solution_Manual.pdf inquire
LT008 Lenti-Pac 293Ta Cell Line (1.5 x 106 cells) 293Ta Lentiviral packaging cell line http://www.genecopoeia.com/wp-content/uploads/2017/06/293ta_packaging_cell_line.pdf inquire
MP001 Mycoplasma PCR detection kit (50 rxns) Quick detection of mycoplasma contamination in cell culture or virus Mycoplasma PCR Detection Kit inquire

 
 

TRE stable cell lines

GeneCopoeia's TRE stable cell lines carry stably integrated TREs, providing you with powerful tools for applications such as drug candidate screening, drug candidate validation, and gene knockout/knockdown analysis.

Advantages

  • Convenience. Avoid spending 2 or more months constructing and validating a stable cell line.
  • Next-day shipping.

 

微信截图_20160630123352

 

Figure 1. Analysis of TRE activity in GeneCopoeia TRE stable cell line. Human cell line HEK293T stably expressing PKC-Luc (cat. # SL401) and HEK293T/NEG-Luc cells (cat. # SL404; negative control) were dispensed into a 96 well plate and grown for 24 hours. Cells were then treated with 0,  0.3,  0.5,  1,  3,  5,  10,  or 15 ng/mL of phorbol 12-myristate 13-acetate (PMA) for 18 hours, followed by analysis for Gaussia luciferase (GLuc) activity.

 

Buy Catalog# Product

Selection marker

Size

Price (academic)

Price (industry)

SL401

Human cell line HEK293T stably expressing PKC-GLuc

Puromycin

2×10^6 cells/vial

$945

$1945

SL402

Human cell line HEK293T stably expressing NFAT-GLuc

Puromycin

2×10^6 cells/vial

$945

$1945

SL403

Human cell line HEK293T stably expressing TCF/LEF-GLuc

Puromycin

2×10^6 cells/vial

$945

$1945

SL404

Human cell line HEK293T stably expressing NEG-GLuc

Puromycin

2×10^6 cells/vial

$945

$1945

 

Related Products

GLuc-ON™ TRE reporter clones

 

Luciferase assay kits

Secrete-Pair Luminescence Assay Kits

Buy Catalog# Product Description Price
LF032 Secrete-Pair Dual Luminescence Assay Kit (300 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF031 Secrete-Pair Dual Luminescence Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF062 Secrete-Pair Gaussia Luciferase Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) inquire
LF061 Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) inquire
LF033 Secrete-Pair Dual Luminescence Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire